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Activation-dependent and activation-independent localisation of calmodulin to the mitotic apparatus during the first cell cycle of the Lytechinus piçtus embryo.

Published online by Cambridge University Press:  26 September 2008

Martin Wilding ,
Katalin Török  and
Michael Whitaker
Martin Wilding*
Affiliation:
Department of Physiology, University College London, London, UK.
Katalin Török
Affiliation:
Department of Physiology, University College London, London, UK.
Michael Whitaker
Affiliation:
Department of Physiology, University College London, London, UK.
*
Michael Whitaker, Department of Physiological Sciences, University of Newcastle, Medical School, Newcastle upon Tyne NE2 4HH, UK.
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Summary

We have used confocal microscopy and a fluorescent calmodulin probe to examine the mechanism of localisation of calmodulin during the first cell cycle of the sea urchin zygote. Using fluoresceincalmodulin, calmodulin can be observed within the nucleus and interphase astral microtubule arrays as cells approach mitosis. During mitosis, calmodulin redistributes to the mitotic apparatus and to condensed chromosomes. Quantitative analysis with reference to a control dye (fluorescein-dextran) shows that the distribution of calmodulin is specific. We used a competitive inhibitor of calcium-dependent calmodulin binding (Trp-peptide; Török & Trentham (1994) Biochemistry 33, 12807–20) to test whether the cell cycle localisation of calmodulin was due to its binding to targets on activation. The Trp-peptide eliminates localisation of calmodulin within the nucleus. However, microtubule localisation persists in the presence of the Trp-peptide. These data show that calmodulin can localise by calcium (and hence activation)-dependent as well as calcium-independent mechanisms. This suggests that distinct mechanisms of localisation may be involved in the regulation of the differential functions of calmodulin, at least during the cell cycle.

Keywords

CalciumCalmodulinFluorescenceMitosisTa-calmodulim
Type
Article
Information
Zygote , Volume 3 , Issue 3 , August 1995 , pp. 219 - 224
Copyright
Copyright © Cambridge University Press 1995

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