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Activation of bovine oocytes penetrated after germinal vesicle breakdown

Published online by Cambridge University Press:  26 September 2008

Lalantha R. Abeydeera ,
Kiyoshi Okuda  and
Koji Niwa
Lalantha R. Abeydeera
Affiliation:
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japan
Kiyoshi Okuda
Affiliation:
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japan
Koji Niwa*
Affiliation:
Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama, Japan
*
K. Niwa, Division of Animal science and Technology, Faculty of Agriculture, Okayama University, Okayama 700, Japan. Telephone: 086-251-8328. Fax: 086-254-0714.
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Summary

The present study was designed to examine the ability of bovine oocytes, after germinal vesicle breakdown (GVBD), to be activated by sperm penetration and the sequence of sperm nuclear transformation. Bovine oovytes cultured for 8 h in maturation medium (tissue culture medium TCM-199 containing 10% fetal calf serum) were inseminated in Brackett and Oilphant's medium supplemented with bovine serum albumin (10 mg/ml), caffeine (5mM) and heparin (10 μg/ml). When oocytes were transferrred to the maturation medium 8 h after insemination and additionally cultured for 5−40 h at 39°C in 5% CO2 in air, 71−76% of oocytes were penetrated and polyspermy (67–75%) was common. The proportions of penetrated oocytes that were activated significantly increased with the lapse of the additional culture time, reaching 88% and 87% by 25 and 40 h after additional culture, respectively. When compared with unpenetrated oocytes, signifcantly higher proportions of penetrated oocytes, reached metaphase II or beyond 15 and 25 h after additional culture. After penetration, sperm nuclei were transformed into metaphase chromosomes and then to telophase chromomes before the formation of male pronuclei. These results provide evidence that bovine oocytes acquire the ability to respond to sperm-mediated activation soon after GVBD.

Keywords

BovineGerminal vesicle breakdownOocyte activationOocytesSperm penetration
Type
Article
Information
Zygote , Volume 2 , Issue 4 , November 1994 , pp. 273 - 279
Copyright
Copyright © Cambridge University Press 1994

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