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Supplementation of kaempferol to in vitro maturation medium regulates oxidative stress and enhances subsequent embryonic development in vitro

Published online by Cambridge University Press:  30 October 2019

Yuhan Zhao Open the ORCID record for Yuhan Zhao [Opens in a new window] ,
Yongnan Xu ,
Yinghua Li ,
Qingguo Jin ,
Jingyu Sun ,
Zhiqiang E  and
Qingshan Gao
Yuhan Zhao
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
Yongnan Xu
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
Yinghua Li
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
Qingguo Jin
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
Jingyu Sun
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
Zhiqiang E
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
Qingshan Gao*
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000 China; and Engineering Research Centre of Yanbian Yellow Cattle Resources Reservation, Yanji, 133000China
*
Author for correspondence: Qingshan Gao, College of Agriculture, Yanbian University, Yanji City, Jilin Province, China. Tel: +86 0433 2436435. Fax: +86 433 243 5638. E-mail: [email protected]
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Summary

Kaempferol (KAE) is one of the most common dietary flavonols possessing biological activities such as anticancer, anti-inflammatory and antioxidant effects. Although previous studies have reported the biological activity of KAE on a variety of cells, it is not clear whether KAE plays a similar role in oocyte and embryo in vitro culture systems. This study investigated the effect of KAE addition to in vitro maturation on the antioxidant capacity of embryos in porcine oocytes after parthenogenetic activation. The effects of kaempferol on oocyte quality in porcine oocytes were studied based on the expression of related genes, reactive oxygen species, glutathione and mitochondrial membrane potential as criteria. The rate of blastocyst formation was significantly higher in oocytes treated with 0.1 µm KAE than in control oocytes. The mRNA level of the apoptosis-related gene Caspase-3 was significantly lower in the blastocysts derived from KAE-treated oocytes than in the control group and the mRNA expression of the embryo development-related genes COX2 and SOX2 was significantly increased in the KAE-treated group compared with that in the control group. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased after KAE treatment. Mitochondrial membrane potential (ΔΨm) was increased and the activity of Caspase-3 was significantly decreased in the KAE-treated group compared with that in the control group. Taken together, these results suggested that KAE is beneficial for the improvement of embryo development by inhibiting oxidative stress in porcine oocytes.

Keywords

AntioxidantEmbryonic developmentKaempferolParthenogeneticPorcine
Type
Research Article
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 59 - 64
Copyright
© Cambridge University Press 2019 

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