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Observation of fresh Bos indicus embryos comparing stereoscopic and phase contrast microscopy

Published online by Cambridge University Press:  21 September 2012

M.E. Gutiérrez ,
C.S. Galina ,
N. Moreno-Mendoza ,
M.A. Alarcón ,
B. Godínez  and
M.A. Lammoglia
M.E. Gutiérrez
Affiliation:
Departamento de Reproducción Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Avenida Universidad 3000 Ciudad Universitaria 04510, MéxicoD.F.
C.S. Galina
Affiliation:
Departamento de Reproducción Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Avenida Universidad 3000 Ciudad Universitaria 04510, MéxicoD.F.
N. Moreno-Mendoza
Affiliation:
Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico.
M.A. Alarcón
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Tuxpan, Universidad Veracruzana, Mexico.
B. Godínez
Affiliation:
Departamento de Reproducción Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Avenida Universidad 3000 Ciudad Universitaria 04510, MéxicoD.F.
M.A. Lammoglia*
Affiliation:
Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana. Km 7.5 carr. Tuxpan-Tampico, Túxpan, Ver.Mexico. CP 92800. e-mail: [email protected]
*
All correspondence to: Miguel Ángel Lammoglia Villagómez. Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana. Km 7.5 carr. Tuxpan-Tampico, Túxpan, Ver.Mexico. CP 92800. e-mail: [email protected]
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Summary

The precision of embryo evaluation using stereoscopic microscopy (SM) and inverted phase contrast microscopy (PCM) was compared in 20 Bos indicus cows superovulated at two different times of the year. In total, 118 embryos were collected and classified according to their developmental stage and quality by two independent evaluators using SM and inverted PCM. Cohen's kappa coefficient was used to determine concordance between SM and PCM observations. A good level of agreement (k = 0.616) was found for quality level, and a moderate one (k = 0.464) for developmental stage, particularly at the morula stage. Using the TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labelling) technique, concordance level was deemed to be low with the SM (k = 0.169), and poor with the PCM (k = 0.217). Differences in concordance levels were also found between observations made at the two times of year, 78 embryos were evaluated in the rainy season when concordance level was good (k = 0.68), in contrast to the 40 embryos evaluated in the dry season when agreement was found to be poor (k = 0.24). In conclusion, inverted PCM was somewhat more effective for evaluating embryos, particularly at the morula stage. However, considering the high cost of an inverted PCM, the differences observed do not justify its purchase for routine embryo evaluation.

Keywords

ApoptosisBos indicusEmbryo qualityInverted phase contrast microscopeMorphologic evaluationTUNEL
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 187 - 194
Copyright
Copyright © Cambridge University Press 2012 

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