Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-02T23:55:54.918Z Has data issue: false hasContentIssue false
  • English
  • Français

Nuclear Sm antigens in the sperm of different organisms

Published online by Cambridge University Press:  26 September 2008

Fernando Delgadoa ,
Monica Brito ,
Ilona I. Concha ,
Ricardo Schroeder  and
Luis O. Burzio
Fernando Delgadoa
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Monica Brito
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Ilona I. Concha
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Ricardo Schroeder
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
Luis O. Burzio*
Affiliation:
Instituto de Bioquímica, Facultad de Ciencias Universidad Austral de Chile, Valdivia, Chile.
*
Luis O.Burzio, Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, PO Box 567, Valdiva, Chile. Fax: 0056-63-219410.
Get access Rights & Permissions [Opens in a new window]

Abstract

Summary

Immunoblot analysis of sperm protein from several species revealed the presence of polypeptides recognised by anti-Sm sera obtained from patients with systemic lupus erythematosus. Immunoreactive polypeptides in human, bull, mouse and rat sperm were identified as protein B', B and D as compared with the Sm polypeptides of HeLa cells. In the sperm of rooster, the teleost fish Cyprinus carpio and the mussel Choromytilus chorus, the immunoreactive polypeptide profile was more complex. To ascertain the sperm origin of the Sm antigens, immunolocalisation with anti-Sm serum was carried out. The results demonstrated that in all the specied studied staining was confinde to the sperm nucleus, confirming that some polypeptides of the small nuclear ribonnucleoprotein complex are present in the gamete.

Keywords

Anti-Sm serumSm antigenssnRNPsSperm antigens
Type
Article
Information
Zygote , Volume 2 , Issue 3 , August 1994 , pp. 227 - 235
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bellve, A.R. & O'Brien, D.A. (1983). The mammalian spermatozoon: Structure and Temporal Assembly. In: Mechanism and Control of Fertilization, ed. Hartman, J.F., pp. 55–137. New York: Academic Press.Google Scholar
Brito, M.Figueroa, J.Vera, J.C.Cortés, P.Hott, R. & Burzio, L.O. (1986). Phosphoproteins are structural components of bull sperm outer dense fiber. Gamete Res. 15, 327–36.CrossRefGoogle Scholar
Brito, M.Concha, I.I.Pessot, C.Mezzano, S. & Burzio, L.O. (1992). Small nuclear ribonucleoprotein in rat sperm. Comp. Biocheni. Physiol. 103, 197–200.Google ScholarPubMed
Burzio, L.O., Guttierrez, E., de la Fuente, E.Saez, C., Brito, M., Pardo, J., Burzio, L.A., Burzio, V.A. & Weiss, R. (1989).Marine bioadhesives: projections in medicine and industry. In: Current Topics in Marine Biotechnology, ed. S., Miyachi, I., Karube & Ishida, Y., pp. 353–6. Tokyo: The Japanese Society for Marine Biotechnology.Google Scholar
Burzio, L.O., Yanez, A.Pessot, C.Urzua, U.Brito, M.Concha, I.I. & Zarraga, A.M. (1991). Characteristics of the sperm RNA. In: Comparative Spermatology 20 Years After, ed. Baccetti, B., pp. 13–17. New York: Raven Press.Google Scholar
Bush, H., Reddy, R., Rothblum, L. & Choi, Y.C. (1982). SnRNAs, SnRNPs and RNA processing. Annu. Rev. Biochem. 51, 617–54.CrossRefGoogle Scholar
Concha, I.I. (1994). SnRNP components are present in the sperm nucleus. PhD thesis, Universidad Austral de Chile.Google Scholar
Concha, I.I., Urzua, U., Yanez, A., Schroeder, R., Pessot, C. & Burzio, L.O. (1993). U1 and U2 snRNA are localized in the sperm nucleus. Exp. Cell Res. 204, 378–81.CrossRefGoogle Scholar
Courtens, J.L., Biggiogera, M., Leser, G., Martin, T.E. & Fakan, S. (1991). Immunogold localization of snRNPs and hnRNPs in the perinuclear substance of rabbit, bull, ram and boar late spermatids and spermatozoa. In: Comparative Spermatology 20 Years After, ed. Baccetti, B., pp. 31–4. New York: Raven Press.Google Scholar
Geremia, R., Boitani, L., Conti, M. & Monesi, V. (1977). RNA synthesis in spermatocyte and spermatids and preservation of meiotic RNA during spermiogenesis in the mouse. Cell Differ. 5, 343–55.CrossRefGoogle ScholarPubMed
Hecht, N.B. (1989). Molecular biology of structural chromosomal proteins of the mammalian testis. In: Molecular Biology of Chromosome Function, ed. K.W., Adolph, pp. 396–420. Heidelberg: Springer.CrossRefGoogle Scholar
Hinterberger, M., Pettersson, I. & Steitz, J.A. (1983). Isolation of small nuclear ribonucleoproteins containing U1, U2, U4, U5 and U6 RNAs. J. Biol. Chem. 258, 2604–13.CrossRefGoogle ScholarPubMed
Kierszenbaum, A.L. & Tres, L. (1975). Structural and transcriptional features of the mouse spermatids genome. Tissue Cell Biol. 65, 258–70.Google ScholarPubMed
Laemmli, U.K. (1970). Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227, 680–5.CrossRefGoogle ScholarPubMed
Luhrmann, R. (1988). snRNP proteins. In: Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles, ed. M.L., Birnstiel, pp. 41–99. Heidelberg:Springer.Google Scholar
McAllister, G., Amara, S.G. & Lerner, M.R. (1988). Tissuespecific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N. Proc Nail Acad. Sci. USA 85, 5296–300.CrossRefGoogle ScholarPubMed
Padget, R.A., Grabowski, P.J., Konarska, M., Seiler, S. & Sharp, P.A. (1986). Splicing of messenger RNA precursors. Annu. Rev. Biochem. 55, 1119–50.Google Scholar
Pardo, J., Gutierrez, E., Saez, C., Brito, M. & Burzio, L.O. (1990). Purification of adhesive proteins from mussels. Protein Expression Purification 1, 147–50.Google Scholar
Pessot, C.A., Brito, M., Figueroa, J., Concha, I.I., Yanez, A. & Burzio, L.O. (1989). Presence of RNA in the sperm nucleus. Biochem. Biophys. Res. Commun. 158, 272–8.CrossRefGoogle Scholar
Robert, D., Bajon, C., Roland, F. & Blaize, A. (1991). Nuclear organization in motile sperm cells of fern, and mosses. In: Comparative Spermatology 20 Years After, ed. B., Baccetti, pp. 73–6. New York: Raven Press.Google Scholar
Rodman, T.C., Litwin, S.D., Romani, M. & Vidali, G. (1979). Life history of mouse sperm protein. J. Cell Biol. 80, 605–20.Google Scholar
Roth, J., Bendayan, M. & Orci, L. (1978). Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J. Histochem. Cytochem. 26, 1074–81.CrossRefGoogle ScholarPubMed
Tan, E.M. (1982). Autoantibodies to nuclear antigens (ANA):their immunobiology and medicine. Adv. Immu nol. 33, 167–240.CrossRefGoogle ScholarPubMed
Tsang, B., Peralta, J. & Simons, A. (1983). Enzyme-linked immuno-electrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 92, 377–91.CrossRefGoogle Scholar
Vera, J.C., Brito, M., Zuvic, T. & Burzio, L.O. (1984). Polypeptide composition of rat sperm outer dense fibers: a simple procedure to isolate the fibrillar complex. J. Biol. Chem. 259, 5970–7.Google Scholar