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The influence of ploidy on the distribution of cells in chimaeric mouse blastocysts

Published online by Cambridge University Press:  26 September 2008

Clare A. Everett  and
John D. West
Clare A. Everett*
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, Edinburgh, UK
John D. West
Affiliation:
Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, Edinburgh, UK
*
Dr Clare A. Everett, Deparment of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK. Telephone: +44 (131)-229-2575. Fax: +44 (131)-229-2408. e-mail:[email protected].
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Summary

Previous studies of tetraploid↔diploid mouse chimaeras and mosaics have revealed that tetraploid cells do not contribute equally to all tissues of the conceptus. In this study we have shown that, within 30 h of aggregating cleavage stage embryos, tetraploid cells were non-randomly distributed among different tissues of the early blastocyst. They were preferentially allocated to the mural trophectoderm regardless of cell size at the time of aggregation. This early effect may underlie the restricted distribution of tetraploid cells at later stages. We have demonstrated for the first time that ploidy can influence the relative position of blastomeres in the preimplantation embryo.

Keywords

BlastocystChimaeraEmbryoMouseTetraploid
Type
Article
Information
Zygote , Volume 4 , Issue 1 , February 1996 , pp. 59 - 66
Copyright
Copyright © Cambridge University Press 1996

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