Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-18T22:42:04.787Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro development of morulae from immature caprine oocytes

Published online by Cambridge University Press:  26 September 2008

Levent Keskintepe ,
Gamal M. Darwish ,
Abdelmoneim I. Younis  and
Benjamin G. Brackett
Levent Keskintepe
Affiliation:
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
Gamal M. Darwish
Affiliation:
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
Abdelmoneim I. Younis
Affiliation:
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
Benjamin G. Brackett*
Affiliation:
Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA
*
Dr B.G. Brackett, Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7389, USA. Tel: (706) 542-3014. Fax: (706) 542-3015.
Get access Rights & Permissions [Opens in a new window]

Summary

The effects of medium supplementation with oestrous goat serum and glycoprotein hormones on caprine oocyte maturation in vitro (IVM) were evidenced by proportions of resulting ova completing in vitro fertilisation (IVF) and development to the morula stage. Oocyte-cumulus complexes (OCCs) were harvested in follicular fluid from 2–5 mm diameter follicles. Oocyte maturation took place during 27 h in TCM-199 supplemented with 20% oestrous goat serum, oestradiol-17β (1.0 μg/ml), and either (a) 0.5 μg FSH/ml, (b) 100 μg LH/ml, (c) 100 μg LH + 0.5 μg FSH/ml, (d) 100 μg hCG + 0.5 μg FSH/ml, (e) 0.5 μg TSH/ml or (f) no added glycoprotein hormone (control). Of 353 immature oocytes cultured in seven experiments, 311 (88.1%) exhibited cumulus expansion at the end of the IVM interval; all normalappearing OCCs were inseminated. In vitro insemination was with ejaculated sperm treated with heparin (10 μg/ml) and caffeine (0.4 μg/ml). Proportions (%) of inseminated ova that were fertilised (cleaved) and that reached the morula stage after IVM with (a) FSH, (b) LH, (c) LH + FSH, (d) hCG + FSH, (e) TSH and (f) no added glycoprotein hormone were (a) 22/52 (42.3%) and 9/52 (17.3%), (b) 25/54 (46.3%) and 14/54 (25.9%), (c) 52/65 (80.0%) and 26/65 (40.0%), (d) 48/78 (61.5%) and 22/78 (28.2%), (e) 14/54 (25.9%) and 4/54 (7.4%), and (f) 11/50 (22.0%) and 1/50 (2.0%), respectively. All treatments yielded better results than IVM with no added glycoprotein hormone. After IVM with added LH + FSH higher proportions of oocytes were fertilised (p<0.05), and higher proportions reached the morula stage (p<0.05) when compared with other treatments.

Keywords

GoatGonadotrophinsIn vitro fertilisationIn vitro maturationMorulae
Type
Article
Information
Zygote , Volume 2 , Issue 2 , May 1994 , pp. 97 - 102
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aggarwal, K.P. & Bhattacharya, N.K. (1982). Nonsurgical transplantation of embryos in goats. Proc 3rd Int. Congr. Goat Prod, and Diseases. Dairy Goat J. [1982], 340.Google Scholar
Brackett, B.G. (1992). In vitro fertilisation in farm animals. In: International Symposium on Embryonic Technology in Domestic Species: Trends in Research and Applications, ed. Lauria, A. & Gandolfi, F., pp. 59–76. Chapel Hill, NC: Portland Press.Google Scholar
Brackett, B.G. & Oliphant, G. (1975). Capacitation of rabbit spermatozoa in vitro. Biol. Reprod. 12, 260–74.CrossRefGoogle ScholarPubMed
Brackett, B.G. & Zuelke, K. (1993). Analysis of factors involved in the in vitro production of bovine embryos. Theriogenology 39, 43–64.CrossRefGoogle Scholar
Brackett, B.G., Bousquet, D., Boice, M.L., Donawick, W.J., Evans, J.F. & Dressel, M.A. (1982). Normal development following in vitro fertilization in the cow. Biol. Reprod. 27, 147–58.CrossRefGoogle ScholarPubMed
Brackett, B.G., Younis, A.I. & Fayrer-Hosken, R.A. (1989). Enhanced viability after in vitro fertilization of bovine oocytes matured in vivo with high concentrations of luteinizing hormone. Fertil. Steril. 52, 319–24.CrossRefGoogle Scholar
Cheng, W.T.K., Moor, R.M. & Polge, C. (1986). In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro. Theriogenology 25, 146.CrossRefGoogle Scholar
Cochran, W.G. & Cox, G.M. (1957). Experimental Designs, 2nd edn., p. 611. New York: Wiley.Google Scholar
Conrod, S.A., Coren, B.R., McBride, B.L., Bowen, M.J. & Kraemer, D.C. (1986). Successful nonsurgical collection of ovine embryos. Theriogenology 25, 149.CrossRefGoogle Scholar
Crozet, N., DeSmedt, J., Ahmed-Ali, M. & Sevellec, C. (1993). Normal development following in vitro oocyte maturation and fertilization in the goat. Theriogenology 39, 206.CrossRefGoogle Scholar
DeSmedt, V., Crozet, N., Ahmed-Ali, M., Martino, A. & Cognie, Y. (1992). In vitro maturation and fertilization of goat oocytes. Theriogenology 37, 1049–60.CrossRefGoogle Scholar
Hanada, A. (1985). In vitro fertilization in goats. Jpn. J. Anim. Reprod. 31, 21–6.Google Scholar
Harper, K.M. & Brackett, B.G. (1992). Bovine blastocyst development after in vitro maturation in a defined medium with epidermal growth factor and low concentration of gonadotropin. Biol. Reprod. 45, 368–378.Google Scholar
Keskintepe, L., Darwish, G.M., Paramio, M.T. & Brackett, B.G. (1993). Caprine oocyte fertilization and development in vitro. Biol. Reprod. 48 (Suppl 1): 171 (abstr. 451).Google Scholar
Kraemer, D.C. (1989). Embryo collection and transfer in small ruminants. Theriogenology 31, 141–8.CrossRefGoogle Scholar
Lindsay, D.R. (1991). Reproduction in the sheep and goat. In Reproduction in Domestic Animals, ed. Cupps, P.T., p. 491. San Diego: Academic Press.Google Scholar
Ling, L., Jufen, Q. & Yong, Z. (1992). In vitro fertilization of goat ovarian oocytes. Theriogenology 37, 247.CrossRefGoogle Scholar
Palmer, E., Bezard, J., Magistrini, M. & Duchamp, G. (1991). In vitro fertilization in the horse: a retrospective study. J. Reprod. Fert. 44, 375–384.Google ScholarPubMed
Parrish, J.J., Susko-Parrish, J.L., Leibfried-Rutledge, M.L., Critser, E.S., Eyestone, W.H. & First, N.L. (1986). Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25, 591–600.CrossRefGoogle ScholarPubMed
Song, H.B. & Iritani, A. (1985). In vitro fertilization of goat follicular oocytes with epididymal spermatozoa capacitated in a chemically defined medium. In: Proceedings of the Third AAAP Animal Science Congress vol. 1, p. 463. Seoul, Korea.Google Scholar
Younis, A.I. & Brackett, B.G. (1992). Thyroid stimulating hormone enhancement of bovine oocyte maturation in vitro. Mol. Reprod. Dev. 31, 144–51.CrossRefGoogle ScholarPubMed
Younis, A.I., Zuelke, K.A., Harper, K.M., Oliveria, M.A.L. & Brackett, B.G. (1991). In vitro fertilization of goat oocytes. Biol. Reprod. 44, 1177–82.CrossRefGoogle ScholarPubMed
Younis, A.I., Keskintepe, L., Mackie, K. & Brackett, B.G. (1992). In vitro maturation and fertilization of Toggenburg oocytes. Theriogenology 37, 330.CrossRefGoogle Scholar
Zuelke, K. & Brackett, B.G. (1990). Luteinizing hormone-enhanced in vitro maturation of bovine oocytes with and without protein supplementation. Biol. Reprod. 43, 784–7.CrossRefGoogle ScholarPubMed