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Glutamine and hypotaurine improves intracellular oxidative status and in vitro development of porcine preimplantation embryos

Published online by Cambridge University Press:  01 November 2007

C. Suzuki ,
K. Yoshioka ,
M. Sakatani  and
M. Takahashi
C. Suzuki*
Affiliation:
Research Team for Production Diseases, National Institute of Animal Health, Kannondai 3–1–5, Tsukuba, Ibaraki 305–0856, Japan.
K. Yoshioka
Affiliation:
Research Team for Production Diseases, National Institute of Animal Health, Kannondai 3–1–5, Tsukuba, Ibaraki 305–0856, Japan.
M. Sakatani
Affiliation:
Research Team for Effects of Climate Change on Agriculture, National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi, Kumamoto 861–1192, Japan.
M. Takahashi
Affiliation:
Research Team for Effects of Climate Change on Agriculture, National Agricultural Research Center for Kyushu Okinawa Region, 2421 Suya, Koshi, Kumamoto 861–1192, Japan.
*
All correspondence to: C. Suzuki, Research Team for Production Diseases, National Institute of Animal Health, Kannondai 3–1–5, Tsukuba, Ibaraki 305–0856, Japan. Tel: +81 29 838 7784. Fax: +81 29 838 7880. e-mail: [email protected]
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Summary

We previously developed an in vitro-production system for porcine embryos and reported that the addition of glutamine (Gln) and hypotaurine (HT) during in vitro culture improved embryo development. This study examined the effects of Gln and HT on in vitro development, intracellular oxidative status and DNA damage of porcine preimplantation embryos. Porcine zygotes produced by in vitro maturation (IVM) and in vitro fertilization (IVF) were cultured until day 2 (day 0 = day of IVF) in porcine zygote medium (PZM) including 2 mM Gln and 5 mM HT, namely PZM-5. On day 2, the cleaved embryos were selected and cultured for 24 h in PZM-5 to which one of the following substances was added: (1) none (control); (2) Gln; (3) HT; or (4) Gln + HT. After 24 h of culture in each medium, the embryos were then returned to PZM-5 and cultured until day 5. Day-5 blastocyst yield was significantly higher in the Gln and Gln + HT groups (p < 0.05) than in the control and HT groups. In addition, Gln + HT significantly increased the total number of cells in blastocysts (p < 0.05) compared with the control. Although the number of cells and the intracellular GSH levels in day-3 cleaved embryos did not differ among treatments, addition of Gln, HT or Gln + HT significantly (p < 0.05) reduced the intracellular H2O2 content and the extent of DNA damage compared with the control. These results indicate that the presence of Gln and HT in PZM-5 from day 2 to day 3 promotes the development of porcine embryos by improvement of intracellular oxidative status.

Keywords

Comet assayIn vitro culturePorcine embryosReactive oxygen species
Type
Research Article
Information
Zygote , Volume 15 , Issue 4 , November 2007 , pp. 317 - 324
Copyright
Copyright © Cambridge University Press 2007

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