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Glucose in a maturation medium with reduced NaCl improves oocyte maturation and embryonic development after somatic cell nuclear transfer and in vitro fertilization in pigs

Published online by Cambridge University Press:  03 March 2021

Yongjin Lee ,
Hanna Lee ,
Joohyeong Lee ,
Seung Tae Lee ,
Geun-Shik Lee  and
Eunsong Lee Open the ORCID record for Eunsong Lee [Opens in a new window]
Yongjin Lee
Affiliation:
College of Veterinary Medicine, Kangwon National University, Chuncheon24341, Korea
Hanna Lee
Affiliation:
College of Veterinary Medicine, Kangwon National University, Chuncheon24341, Korea
Joohyeong Lee
Affiliation:
Institute of Veterinary Science, Kangwon National University, Chuncheon24341, Korea
Seung Tae Lee
Affiliation:
Division of Applied Animal Science, Kangwon National University, Chuncheon24341, Korea
Geun-Shik Lee
Affiliation:
College of Veterinary Medicine, Kangwon National University, Chuncheon24341, Korea
Eunsong Lee*
Affiliation:
College of Veterinary Medicine, Kangwon National University, Chuncheon24341, Korea Institute of Veterinary Science, Kangwon National University, Chuncheon24341, Korea
*
Author for correspondence: Eunsong Lee. College of Veterinary Medicine, Kangwon National University, Chuncheon24341, Korea. Tel: +82 33 250 8670. Fax: +82 33 259 5625. E-mail: [email protected]
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Summary

This study was conducted to examine whether glucose in maturation medium containing reduced NaCl could improve oocyte maturation and embryonic development in pigs. The base medium was bovine serum albumin-free porcine zygote medium (PZM)-3 containing 10% (v/v) pig follicular fluid (FPZM) or 0.1% (w/v) polyvinyl alcohol (PPZM). Using each medium, the effects of NaCl concentrations (108 and 61.6 mM) and 5.56 mM glucose supplementation (designated as PZM108N, PZM108G, PZM61N, and PZM61G, respectively) were examined using a 2 × 2 factorial arrangement. When oocytes were matured in FPZM, glucose supplementation improved nuclear maturation compared with no supplementation, regardless of the NaCl concentrations. FPZM61G showed a higher blastocyst formation compared with FPZM108N and FPZM108G after parthenogenesis (PA). Blastocyst formations of somatic cell nuclear transfer (SCNT) embryos derived from FPZM61N and FPZM61G were higher compared with those of oocytes from FPZM108N. When oocytes were matured in PPZM, glucose added to PPZM108 and PPZM61 increased nuclear maturation compared with no supplementation. However, glucose added to PPZM108 did not alter embryonic development after PA. Additionally, oocytes matured in PPZM61G showed a higher blastocyst formation compared with those from PPZM61N. In SCNT, blastocyst formation was not influenced by glucose supplementation of PPZM108, but was increased by maturation in glucose-supplemented PPZM61. In embryonic development of in vitro fertilization (IVF), oocytes matured in medium with reduced NaCl and glucose showed significantly higher blastocyst formation compared with those matured in PPZM108G. Our results demonstrated that glucose in maturation medium containing 61.6 mM NaCl increased oocyte maturation and embryonic development after PA, SCNT, and IVF.

Keywords

Defined mediumGlucoseNuclear transferOocyte maturationReduced NaCl
Type
Research Article
Information
Zygote , Volume 29 , Issue 4 , August 2021 , pp. 293 - 300
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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Footnotes

*

These two authors contributed equally to this work.

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