Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-24T00:34:00.460Z Has data issue: false hasContentIssue false
  • English
  • Français

Extender supplementation with catalase maintains the integrity of sperm plasma membrane after freezing–thawing of semen from capuchin monkey

Published online by Cambridge University Press:  09 March 2017

Danuza L. Leão ,
Adriel B. Brito ,
Stefânia A. Miranda ,
Karol G. Oliveira ,
Débora V.C. Almeida ,
Regiane R. Santos  and
Sheyla F. S. Domingues
Danuza L. Leão
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Adriel B. Brito
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Stefânia A. Miranda
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Karol G. Oliveira
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil. National Primate Center, Ananindeua, PA, Brazil.
Débora V.C. Almeida
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
Regiane R. Santos*
Affiliation:
Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil
Sheyla F. S. Domingues
Affiliation:
Laboratory of Amazon Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Belém, Pará, Brazil.
*
All correspondence to: Regiane R Santos. Federal University of Pará, Laboratory of Wild Animal Biology and Medicine, BR 316 Km 61, CEP 68740–970, Castanhal, Pará, Brazil. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

We aimed to evaluate the effect of supplementation of ACP-118® extender with the antioxidant catalase (10 and 50 µg/ml) on Sapajus apella sperm motility, vigour, and plasma membrane integrity during the processes of seminal liquefaction, cooling, and freezing. Catalase did not affect any of the evaluated parameters after semen dilution or cooling. Cryopreserved sperm in the presence of 50 µg/ml catalase presented a plasma membrane integrity similar to that fresh sperm, however.

Keywords

AntioxidantCell membraneMonkeySemen
Type
Research Article
Information
Zygote , Volume 25 , Issue 2 , April 2017 , pp. 231 - 234
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agarwal, A., Sharma, R.K., Nallella, K.P., Thomas, A.J. Jr., Alvarez, J.G. & Sikka, S.C. (2006). Reactive oxygen species as an independent marker of male factor infertility. Fertil. Steril. 86, 878–85.Google Scholar
Agarwal, A., Virk, G., Ong, C. & du Plessis, S.S. (2014). Effect of oxidative stress on male reproduction. World J. Men's Health 32, 1–17.Google Scholar
Aitken, R.J. & Baker, M.A. (2006). Oxidative stress, sperm survival and fertility control. Mol. Cel. Endocrinol. 250, 66–9.Google Scholar
Dixson, A.F. & Anderson, M.J. (2002). Sexual selection, seminal coagulation and copulatory plug formation in Primates. Folia Primatol. 73, 63–9.Google Scholar
Dong, Q., Rodenburg, S.E., Huang, C. & VandeVoort, C.A. (2008). Cryopreservation of rhesus monkey (Macaca mulatta) epididymal spermatozoa before and after refrigerated storage. J. Androl. 29, 283–92CrossRefGoogle ScholarPubMed
Dong, Q., Correa, L.M. & Yandevoort, C.A. (2009). Rhesus monkey sperm cryopreservation with TEST-yolk extender in the absence of permeable cryoprotectant. Cryobiology 58, 20–7.Google Scholar
Kefer, J.C., Agarwal, A. & Sabanegh, E. (2009). Role of antioxidants in the treatment of male infertility. Int. J. Urol. 16, 449–57.Google Scholar
Kothari, S., Thompson, A., Agarwal, A. & Plessis, S.S. (2010). Free radicals: their beneficial and detrimental effects on sperm function. Indian J. Exp. Biol. 48, 425–35.Google ScholarPubMed
Leão, D.L., Miranda, S.A., Brito, A.B., Lima, J.S., Santos, R.R. & Domingues, S.F.S. (2015). Efficacious long-term cooling and freezing of I semen in ACP-118®. Anim. Reprod. Sci. 159, 118–23.Google Scholar
McCarthy, M.J. & Meyers, S.A. (2011). Antioxidant treatment in the absence of exogenous lipids and proteins protects rhesus macaque sperm from cryopreservation-induced cell membrane damage. Theriogenology 76, 168–76.CrossRefGoogle ScholarPubMed
Mora-Esteves, C. & Shin, D. (2013). Nutrient supplementation: improving male fertility fourfold. Sem. Reprod. Med. 31, 293–300.Google Scholar
Moubasher, A.E., El Din, A.M.E., Ali, M.E., El-Sherif, W.T. & Gaber, H.D. 2013. Catalase improves motility, vitality and DNA integrity of cryopreserved human spermatozoa. Andrologia 45, 135–9.Google Scholar
Oliveira, K.G., Miranda, S.A., Leão, D.L., Brito, A.B., Santos, R.R. & Domingues, S.F.S. (2011). Semen coagulum liquefaction, sperm activation and cryopreservation of capuchin monkey (Cebus apella) semen in coconut water solution (CWS) and TES–TRIS. Anim. Reprod. Sci. 123, 75–80.CrossRefGoogle ScholarPubMed
Oliveira, K.G., Leão, D.L., Almeida, D.V.C., Santos, R.R & Domingues, S.F.S. (2015). Seminal characteristics and cryopreservation of sperm from the squirrel monkey, Saimiri collinsi . Theriogenology 84, 743–9.CrossRefGoogle ScholarPubMed
Oliveira, K.G., Santos, R.R., Leão, D.L., Queiroz, H.L., Paim, F.P., Vianez-Júnior, J.L.S.G. & Domingues, S.F.S. (2016a). Similarities in testicular and seminal aspects in four squirrel monkeys’ species. Theriogenology 86, 879–87.Google Scholar
Oliveira, K.G., Santos, R.R., Leão, D.L., Brito, A.B., Lima, J.S., Sampaio, W.V. & Domingues, S.F.S. (2016b). Cooling and freezing of sperm from captive, free-living and endangered squirrel monkey species. Cryobiology 72, 283–9.CrossRefGoogle ScholarPubMed
Taylor, K., Roberts, P., Sanders, K. & Burton, P. (2009). Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa. Reprod. BioMed. Online 18, 184–9.Google Scholar
Supplementary material: File

Leão supplementary material

Table S1

Download Leão supplementary material(File)
File 12.6 KB