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Establishment of a basic method for manipulating preantral follicles: effects of retrieval method on in vitro growth of preantral follicles and intrafollicular oocytes

Published online by Cambridge University Press:  01 May 2007

S.T. Lee ,
M.H. Choi ,
S.P. Gong ,
J.Y. Han  and
J.M. Lim
S.T. Lee
Affiliation:
Department of Food and Animal Biotechnology, Seoul National University, Seoul 151-921, Korea.
M.H. Choi
Affiliation:
Department of Food and Animal Biotechnology, Seoul National University, Seoul 151-921, Korea.
S.P. Gong
Affiliation:
Department of Food and Animal Biotechnology, Seoul National University, Seoul 151-921, Korea.
J.Y. Han
Affiliation:
Department of Food and Animal Biotechnology, Seoul National University, Seoul 151-921, Korea.
J.M. Lim*
Affiliation:
Department of Food and Animal Biotechnology, Seoul National University, Seoul 151-921, Korea. Stem Cell Research Center and IRMP, College of Medicine, Seoul National University, Seoul 110-744, Korea.
*
All correspondence to: Jeong Mook Lim, DVM, PhD, Laboratory of Embryology and Gamete Biotechnology, Department of Food and Animal Biotechnology, Seoul National University, Building 200-#4223, Sillim-9 Dong, Seoul 151-921, Korea. Tel: +822 880 4806. Fax: +822 8742555. e-mail: [email protected]
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Summary

The aim of this study was to establish a basic manipulation protocol of preantral follicles for deriving developmentally competent oocytes. Primary, early and late secondary follicles retrieved from the ovaries of 14-day-old F1 (C57BL/6 × DBA2) female mice mechanically or enzymatically were cultured singly and in vitro growth of the follicles and maturation of intrafollicular oocytes were subsequently monitored. A mechanical method retrieved more (p < 0.0001) follicles (339 ± 48 vs. 202 ± 28) than an enzymatic method. However, the enzymatic method collected more singly isolated follicles that could be provided for subsequent culture (102 ± 26 vs. 202 ± 28). When an enzymatic method was employed, early and late secondary follicles required 9 and 6 days for reaching the maximal incidence of the pseudoantral stage. However, primary follicles were not possible to develop into the pseudoantral stage. The optimal duration of oocyte maturation from the onset of follicle culture was 7 days and 5–7 days for early and late secondary follicles, respectively. A general decrease in oocyte diameter (65.2–65.53 μm vs. 75 μm) and zona thickness (5.41–5.74 μm vs. 7.76 μm) was detected in in vitro-derived compared with in vivo-derived matured oocytes. Pronuclear formation was detected in 86–94% of mature oocytes after parthenogenetic activation and no significant difference was detected among groups. These results showed that preantral follicles retrieved by an enzymatic method underwent step-by-step growth in vitro, which could yield mature oocytes.

Keywords

Follicular oocyteIn vitro growthMousePreantral follicleRetrieval
Type
Research Articles
Information
Zygote , Volume 15 , Issue 2 , May 2007 , pp. 109 - 116
Copyright
Copyright © Cambridge University Press 2007

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References

Cortvrindt, R., Smitz, J. & Van Steirteghem, A.C. (1996) In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Hum. Reprod. 11, 2656–66.CrossRefGoogle Scholar
Cortvrindt, R.G., Hu, Y., Liu, J. & Smitz, J.E. (1998a) Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin. Fertil. Steril. 70, 1114–25.Google Scholar
Cortvrindt, R., Hu, Y. & Smitz, J. (1998b) Recombinant luteinizing hormone as a survival and differential factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture. Hum. Reprod. 13, 1292–302.Google Scholar
Cortvrindt, R. & Smitz, J. (1998) Early preantral mouse follicle in vitro maturation: oocyte growth, meiotic maturation and granulosa-cell proliferation. Theriogenology 49, 845–59.CrossRefGoogle ScholarPubMed
Demeestere, I., Delbaere, A., Gervy, C., Van Den Bergh, M., Devreker, F. & Englert, Y. (2002) Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice. Hum. Reprod. 17, 2152–9.CrossRefGoogle ScholarPubMed
Dong, J., Albertini, D.F., Nishimori, K., Kumar, T.R., Lu, N. & Matzuk, M.M. (1996) Growth differentiation factor-9 is required during early ovarian folliculogenesis. Nature 383, 531–35.CrossRefGoogle ScholarPubMed
Elvin, J.A., Yan, C. & Matzuk, M.M. (2000) Oocyte-expressed TGF-β superfamily members in female fertility. Mol. Cell. Endocrinol. 159, 1–5.CrossRefGoogle ScholarPubMed
Eppig, J.J. & Schroeder, A.C. (1989) Capacity of mouse oocytes from preantral follicles to undergo embryogenesis and development to live young after growth, maturation, and fertilization in-vitro. Biol. Reprod. 41, 268–76.CrossRefGoogle ScholarPubMed
Epigg, J.J. & O'Brien, M.J. (1998) Comparison of preimplantation developmental competence after mouse growth and development in vitro and in vivo. Theriogenology 49, 415–22.Google Scholar
Eppig, J.J., O'Brien, M.J., Pendola, F.L. & Watanabe, S. (1998) Factors affecting the developmental competence of mouse oocytes grown in vitro: follicle-stimulating hormones and insulin. Biol. Reprod. 59, 1445–53.Google Scholar
Hubner, K., Fuhrmann, G., Christenson, L.K., Kehler, J., Reinbold, R., De La Fuente, R., Wood, J., Strauss, J.F. 3rd, Boiani, M. & Scholer, H.R. (2003) Derivation of oocytes from mouse embryonic stem cells. Science 300, 1251–6.CrossRefGoogle ScholarPubMed
Lenie, S., Cortvrindt, R., Adriaenssens, T. & Smitz, J. (2004) A reproducible two-step culture system for isolated primary mouse ovarian follicles as single functional units. Bio. Reprod. 71, 1730–8.CrossRefGoogle ScholarPubMed
Liu, J., Van Der Elst, J., Van Den Broecke, R., Dumortier, F. & Dhont, M. (2000) Maturation of mouse primordial follicles by combination of grafting and in vitro culture. Bio. Reprod. 62, 1218–23.CrossRefGoogle ScholarPubMed
Moore, K.L. & Persaud, T.V.N. (1998) Before We Are Born; Essentials of Embryology and Birth Defects. 5th edn, W.B. Saunders, Philadelphia (America).Google Scholar
Nayudu, P.L. & Osborn, S.M. (1992) Factors influencing the rate of preantral and antral growth of mouse ovarian follicles in vitro. J. Reprod. Fertil. 95, 349–62.Google Scholar
Pangas, S.A., Saudye, H., Shea, L.D. & Woodruff, T.K. (2003) Novel approach for the three-dimensional culture of granulose cell–oocyte complexes. Tissue Eng. 9, 1013–21.CrossRefGoogle Scholar
Roy, S.K. & Greenwald, G.S. (1985) An enzymatic method for the dissociation of intact follicles from the hamster ovary: histological and quantitative aspect. Biol. Reprod. 32, 203–15.Google Scholar
Roy, S.K. & Treacy, B.J. (1993) Isolation and long-term culture of human preantral follicles. Fertil. Steril. 59, 783–90.Google Scholar
Roy, S.K. & Greenwald, G.S. (1996) Methods of separation and in-vitro culture of preantral follicles from mammalian ovaries. Hum. Reprod. Update 2, 236–45.Google Scholar
Smitz, J.E. & Cortvrindt, R.G. (2002) The earliest stage of folliculogenesis in vitro. Reproduction 123, 185–202.CrossRefGoogle ScholarPubMed