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Effects of IAA in combination with FSH on in vitro culture of ovine preantral follicles

Published online by Cambridge University Press:  09 July 2009

Sonia H.F. Costa ,
Regiane R. Santos ,
Davide Rondina ,
Evelyn R. Andrade ,
Otávio M. Ohashi ,
Ana Paula R. Rodrigues  and
José R. Figueiredo
Sonia H.F. Costa
Affiliation:
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Regiane R. Santos*
Affiliation:
Faculty of Veterinary Medicine, Utrecht University, Department of Equine Sciences, Veterinary Pharmaceuticals, Pharmacology and Toxicology Division, Yalelaan 114, 3584 CM, Utrecht, The Netherlands.
Davide Rondina
Affiliation:
Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
Evelyn R. Andrade
Affiliation:
State University of Londrina, Londrina, PR, Brazil.
Otávio M. Ohashi
Affiliation:
Federal University of Pará, Faculty of Biology, Belém, Pará, Brazil.
Ana Paula R. Rodrigues
Affiliation:
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
José R. Figueiredo
Affiliation:
LAMOFOPA, Faculty of Veterinary, State University of Ceará, Fortaleza, CE, Brazil.
*
All correspondence to: Regiane Rodrigues dos Santos. Faculty of Veterinary Medicine, Utrecht University, Department of Equine Sciences, Veterinary Pharmaceuticals, Pharmacology and Toxicology Division, Yalelaan 114, 3584 CM, Utrecht, The Netherlands. Tel: +31 30 253 1078. e-mail: [email protected]
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Summary

Ovarian cortical fragments from five adult ewes were in vitro cultured for 1, 3 or 5 days in the presence of minimum essential medium either supplemented or not by follicle-stimulating hormone (FSH) (100 ng/ml) or indole-3-acetic acid (IAA) (10, 20, 40 or 100 ng/ml), alone or in combination. After in vitro culture, ovarian fragments were submitted to follicular isolation and viability test was performed using trypan blue. Addition of IAA (10 ng/ml) to a free-FSH medium resulted in the highest percentages of viable follicles, but was progressively deleterious in higher concentrations (20, 40 and 100 ng/ml) if in absence of FSH. Follicular development was observed only when FSH was added to an IAA-free medium. In conclusion, IAA at a concentration of 10 ng/ml increases follicular survival in vitro. However, at high concentrations (20, 40 or 100 ng/ml), this auxin may be deleterious to preantral follicles, the addition of FSH to the medium being necessary.

Keywords

AntioxidantFSHIVCPhytohormonePreantral follicles
Type
Research Article
Information
Zygote , Volume 18 , Issue 1 , February 2010 , pp. 89 - 92
Copyright
Copyright © Cambridge University Press 2009

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