Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-07T21:24:34.533Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of fulvic acids on goat sperm

Published online by Cambridge University Press:  28 June 2018

Yu Xiao ,
Zhengmu Wu  and
Min Wang
Yu Xiao*
Affiliation:
Reproductive Medical Center, the International Peace Maternity and Child Health Hospital of China Welfare Institute, 910 Hengshan Road, Shanghai, 200030, China
Zhengmu Wu
Affiliation:
Reproductive Medical Center, the International Peace Maternity and Child Health Hospital of China Welfare Institute, 910 Hengshan Road, Shanghai, 200030, China
Min Wang
Affiliation:
Reproductive Medical Center, the International Peace Maternity and Child Health Hospital of China Welfare Institute, 910 Hengshan Road, Shanghai, 200030, China
*
All correspondence to: Yu Xiao. Reproductive Medical Center, the International Peace Maternity and Child Health Hospital of China Welfare Institute, 910 Hengshan Road, Shanghai, 200030, China. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Summary

The effects of adding fulvic acids (FAs) to semen extenders on the quality parameters of frozen–thawed goat buck spermatozoa remain undetermined. Buck semen samples collected from six mature goat bucks once a week were diluted with Tris–egg yolk-based extenders. The diluted semen samples were supplemented with FAs (0.2, 0.4 and 0.6%, w/w), cryopreserved, and evaluated for sperm-quality parameters. Addition of FAs to the extender increased progressive motility, acrosome integrity, membrane integrity, and superoxide dismutase and catalase activities and decreased percentage abnormality and sperm malondialdehyde level compared with the control group. However, excessive FA addition (>0.4%, w/w) to semen extenders did not improve the efficiency. The results indicated that FAs could be a promising cryoprotectant for goat buck sperm.

Keywords

CryopreservationFulvic acidsGoatSemen extenderSperm
Type
Research Article
Information
Zygote , Volume 26 , Issue 3 , June 2018 , pp. 220 - 223
Copyright
Copyright © Cambridge University Press 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, Z., Anzar, M., Shahab, N., Ahmad, S. & Andrabi, M.H. (2003). Sephadex and Sephadex ion exchange filtration improves the quality and freezability of low-grade buffalo semen ejaculates. Theriogenology 59, 1189–202.CrossRefGoogle ScholarPubMed
Aiken, G.R., McKnight, D.M., Wershaw, R.L. & MacCarthy, P. (1985). Humic Substances in Soil, Sediment and Water: Geochemistry, Isolation and Characterization, 1st edn. New York: Wiley.Google Scholar
Bai, H.X., Chang, Q.F., Shi, B.M. & Shan, A.S. (2013). Effects of fulvic acid on growth performance and meat quality in growing-finishing pigs. Livest. Sci. 158, 118–23.Google Scholar
Bearden, H.J. & Fuquay, J.W. (1997). Semen evaluation. In Applied Animal Reproduction, 4th edn (eds Bearden, H.J. & Fuquay, J.W.), pp. 158–69. New Jersey: Prentice Hall, Upper Saddle River.Google Scholar
Che, R.Q., Huang, L., Xu, J.W., Zhao, P., Li, T., Ma, H.X. & Yu, X.Y. (2017). Effect of fulvic acid induction on the physiology, metabolism, and lipid biosynthesis-related gene transcription of Monoraphidium sp. FXY-10. Bioresource Technol. 227, 324–34Google Scholar
Chang, Q.F., Bai, H.X., Shi, B.M., Shan, A.S., Wei, C.Y., Yu, C.Q. & Tong, B.S. (2013). Effects of dietary FA on the growth performance, serum biochemical indices, routine blood parameter and immunity of growing swine. Chin. J. Anim. Nutr. 25, 1836–42.Google Scholar
Çimrin, K.M., Türkmen, Ö., Turan, M. & Tuncer, B. (2010). Phosphorus and humic acid application alleviate salinity stress of pepper seedling. Afr. J. Biotechnol. 9, 5845–51.Google Scholar
Flohe, L. & Otting, F. (1984). Superoxide dismutase assays. Methods Enzymol. 105, 93–104.Google Scholar
Gao, Y., He, J., He, Z.L., Li, Z.W., Zhao, B., Mu, Y., Lee, J.Y. & Chu, Z.J. (2017). Effects of fulvic acid on growth performance and intestinal health of juvenile loach Paramisgurnus dabryanus (Sauvage). Fish. Shellfish Immun. 62, 47–56.Google Scholar
Gutiérrez-Dagnino, A., Fierro-Coronado, J.A., Álvarez-Ruí, P., del Carmen Flores-Miranda, M., Miranda-Saucedo, S., Medina-Beltrán, V. & Escamilla-Montes, R. (2015). Effect of inulin and fulvic acid on survival, growth, immune system, and WSSV prevalence in Litopenaeus vannamei. Lat. Am. J. Aquat. Res. 43, 912–21.CrossRefGoogle Scholar
Giaretta, E., Estrada, E., Bucci, D., Spinaci, M., Rodríguez-Gil, J.E. & Yeste, M. (2015). Combining reduced glutathione and ascorbic acid has supplementary beneficial effects on boar sperm cryotolerance. Theriogenology 83, 399–407.Google Scholar
Goth, L. (1991). A simple method for determination of serum catalase activity and revision of reference range. Clin. Chim. Acta. 196, 143–51.Google Scholar
Hu, J.H., Li, Q.W., Li, G., Jiang, Z.L., Bu, S.H., Yang, H. & Wang, L.Q. (2009). The cryoprotective effect of trehalose supplementation on boar spermatozoa quality. Anim. Reprod. Sci. 112, 107–18. (Retracted)Google Scholar
Hu, J.H., Sun, X.Z., Li, Q.W., Zhang, T., Hu, X.C., Hu, J.H. & Wan, L.Q. (2013). The effect of Laminaria japonic polysaccharide on sperm characteristics and biochemical parameters in cryopreserved boar sperm. Anim. Reprod. Sci. 139, 95–100.Google Scholar
Huck, J.A., Porter, N. & Bushed, M.E. (1991). Effect of humates on microbial activity. J. Gen. Microbiol. 137, 2321–9.CrossRefGoogle Scholar
Islam, K.M.S., Schumacher, A. & Groop, J.M. (2005). Humic acid substances in animal agriculture. Pakistan J. Nutr. 4, 126–34.Google Scholar
Janos, P. (2003). Separation methods in the chemistry of humic substances. J. Chromatogr. A. 983, 1–18.Google Scholar
Jayasooriya, R.G.P.T., Dilshara, M.G., Kang, C.H., Lee, S., Choi, Y.H., Jeong, Y.K. & Kim, G.Y. (2016). Fulvic acid promotes extracellular anti-cancer mediators from RAW 264.7 cells, causing to cancer cell death in vitro. Int. Immunopharmacol. 36, 241–8.Google Scholar
Liu, C.H., Dong, H.B., Ma, D.L., Li, Y.W., Han, D., Luo, M.J., Chang, Z.L. & Tan, J.H. (2016). Effects of pH during liquid storage of goat semen on sperm viability and fertilizing potential. Anim. Reprod. Sci. 164, 47–56.Google Scholar
Nur, Z., Zik, B., Ustuner, B., Sagirkaya, H. & Ozguden, C.G. (2010). Effects of different cryoprotective agents on ram sperm morphology and DNA integrity. Theriogenology 73, 1267–75.Google Scholar
Plaza, C., García-Gil, J.C., Polo, A., Senesi, N. & Brunetti, G. (2005). Proton binding by humic and fulvic acids from pig slurry and amended soils. J. Environ. Qual. 34, 1131–7.CrossRefGoogle ScholarPubMed
Qian, L., Yu, S.J. & Zhou, Y. (2016). Protective effect of hyaluronic acid on cryopreserved boar sperm. Int. J. Biol. Macromol. 87, 287–9.Google Scholar
Vidal, A.H., Batista, A.M., da Silva, E.C.B., Gomes, W.A., Pelinca, M.A., Silva, S.V. & Guerra, M.M.P. (2013). Soybean lecithin-based extender as an alternative for goat sperm cryopreservation. Small Rumin. Res. 109, 47–51.Google Scholar
Yeste, M., Estrada, E., Pinart, E., Bonet, S., Miró, J. & Rodríguez-Gil, J.E. (2014). The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability. Cryobiology 68, 251–61.Google Scholar
Yang, H.L., Chiu, H.C. & Lu, F. (1996). Effects of humic acid on the viability and coagulant properties of human umbilical vein endothelial cells. Am. J. Hematol. 51, 200–6.Google Scholar