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Effects of different polyvinylpyrrolidone concentrations on intracytoplasmic sperm injection

Published online by Cambridge University Press:  14 January 2020

Ding Ding ,
Qiushuang Wang ,
Xinyuan Li ,
Beili Chen ,
Weiwei Zou ,
Dongmei Ji ,
Yan Hao ,
Rufeng Xue ,
Huijuan Zou  and
Zhaolian Wei
...Show all authors
Ding Ding
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China NHC Key Laboratory of study on abnormal gametes and reproductive tract (Anhui Medical University), No. 81 Meishan Road, Hefei230032, Anhui, China Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, No. 81 Meishan Road, Hefei230032, Anhui, China
Qiushuang Wang
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China NHC Key Laboratory of study on abnormal gametes and reproductive tract (Anhui Medical University), No. 81 Meishan Road, Hefei230032, Anhui, China Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, No. 81 Meishan Road, Hefei230032, Anhui, China
Xinyuan Li
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Beili Chen
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Weiwei Zou
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Dongmei Ji
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Yan Hao
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Rufeng Xue
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Huijuan Zou
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Zhaolian Wei
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Ping Zhou
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Yunxia Cao
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China Anhui Province Key Laboratory of Reproductive Health and Genetics, No. 81 Meishan Road, Hefei230032, Anhui, China Biopreservation and Artificial Organs, Anhui Provincial Engineering Research Center, Anhui Medical University, No. 81 Meishan Road, Hefei230032, Anhui, China
Zhiguo Zhang*
Affiliation:
Reproductive Medicine Center, Department of Obstetrics and Gynecology, the First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei230022, Anhui, China NHC Key Laboratory of study on abnormal gametes and reproductive tract (Anhui Medical University), No. 81 Meishan Road, Hefei230032, Anhui, China Key Laboratory of Population Health Across Life Cycle (Anhui Medical University), Ministry of Education of the People’s Republic of China, No. 81 Meishan Road, Hefei230032, Anhui, China
*
Author for correspondence: Zhiguo Zhang. Reproductive Medicine Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei230022, China. Tel:/Fax: +86 181 5510 6683. E-mail: [email protected]
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Summary

To explore whether different polyvinylpyrrolidone (PVP) concentrations affect the results of intracytoplasmic sperm injection (ICSI), a prospective study was conducted for 194 couples undergoing 210 ICSI therapy cycles. These cycles were divided into three groups (10, 7 and 5% groups) using the corresponding concentration of PVP for sperm immobilization. The main outcome measures were analyzed. Results indicated that, with a decrease in PVP concentrations, all of the main outcome measures increased. In particular, the high-quality cleavage embryo rate in the 7% group was significantly lower than in the 5% group (P < 0.01), and the cleavage, high-quality cleavage embryo, and high-quality blastocyst rates in the 5% group were significantly higher than those in the 10% group (all P < 0.001). For high-/intermediate-quality semen, all of the main outcome measures were significantly increased with 5% PVP. For the poor-quality semen, only the high-quality cleavage embryo and high-quality blastocyst rates were significantly higher in the 5% group. Therefore, lowering PVP concentrations greatly promoted the development of embryos in ICSI cycles, with an optimal concentration of 5% for ICSI.

Keywords

Polyvinylpyrrolidoneconcentrationintracytoplasmic sperm injectionsemendevelopment
Type
Research Article
Information
Zygote , Volume 28 , Issue 2 , April 2020 , pp. 148 - 153
Copyright
© Cambridge University Press 2020

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Footnotes

*

These authors contributed equally to this work.

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