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Effect of medium conditioned with rat hepatoma BRL cells on ‘2-cell block’ of random-bred mouse embryos cultured in vitro

Published online by Cambridge University Press:  01 May 2009

Masayuki Kobayashi ,
Yoshinori Terawaki ,
Koichi Saito ,
Kano Kasuga  and
Ikuo Kojima
Masayuki Kobayashi*
Affiliation:
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Akita 010–0195, Japan. Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Yoshinori Terawaki
Affiliation:
Dairy Science Institute, Rakuno Gakuen University, Ebetsu, Hokkaido 064–8501, Japan.
Koichi Saito
Affiliation:
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Kano Kasuga
Affiliation:
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
Ikuo Kojima
Affiliation:
Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.
*
All correspondence to: Masayuki Kobayashi, Department of Biotechnology, Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Akita 010–0195, Japan. Fax: +81 18 872 1676. e-mail: [email protected]
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Summary

The phenomenon of developmental arrest at the 2-cell stage of zygotes obtained from certain mouse strains during in vitro culture is known as the 2-cell block. The effect of conditioned medium (CM) with rat hepatoma BRL cells on the 2-cell block of CD-1 mouse zygotes was investigated in comparison with that of CM with rat hepatoma Reuber H-35 cells. In control medium with EDTA, 75.4% of 2-cell embryos developed to the 4- to 8-cell stages. In the same conditions, the BRL Mr <10000 fraction inhibited the development of 2-cell embryos to the 4- to 8-cell stages (57.7%), although the inhibition by this fraction was weaker than by the Reuber Mr <10000 fraction (19.8%). As a result of reversed-phase column chromatography, a 2-cell stage specific inhibitor of the cleavage of mouse embryos (Fr.B-25), which separated into the Mr <10000 fraction of the Reuber CM, was detected at a low level in the BRL Mr <10000 fraction. On the other hand, the Mr >10000 fraction of BRL CM accelerated the development of the embryos (90.3%). This beneficial effect was also evident even in the absence of EDTA. RT-PCR analysis revealed that mRNAs encoding the β-A or β-B subunit of activins (Mr ~29000), which are well characterized cytokines that act as releasers of the 2-cell block, were expressed in BRL cells. These results indicate that BRL cells synthesize Fr.B-25 at low levels, and that activins contained in the BRL CM probably contributed to overcoming the 2-cell block of CD-1 zygotes cultured in vitro.

Keywords

ActivinDevelopmentEmbryoHepatomaIn vitro culture2-cell block
Type
Research Article
Information
Zygote , Volume 17 , Issue 2 , May 2009 , pp. 169 - 174
Copyright
Copyright © Cambridge University Press 2009

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References

Abramczuk, J., Solter, D. & Koprowski, H. (1977). The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined medium. Dev. Biol. 61, 378–83.CrossRefGoogle ScholarPubMed
Babalola, G.O. & Schultz, R.M. (1995). Modulation of gene expression in the preimplantation mouse embryo by TGF-α and TGF-β. Mol. Reprod. Dev. 41, 133–9.CrossRefGoogle ScholarPubMed
Brigstock, D.R., Heap, R.B. & Brown, K.D. (1989). Polypeptide growth factors in uterine tissues and secretions. J. Reprod. Fertil. 85, 747–58.CrossRefGoogle ScholarPubMed
Cary, N. (1993). SAS/STAT User's Guide. Tokyo, Japan: Statistical Analysis System Institute Inc. Japan.Google Scholar
Dardik, A. & Schultz, R.M. (1991). Blastocoel expansion in the preimplantation mouse embryo: stimulatory effect of TGF-α and EGF. Development 113, 919–30.CrossRefGoogle ScholarPubMed
Esch, F.S., Shimasaki, S., Cooksey, K., Mercado, M., Mason, A.J., Ying, S.Y., Ueno, N. & Ling, N. (1987). Complementary deoxyribonucleic acid (cDNA) cloning and DNA sequence analysis of rat ovarian inhibins. Mol. Endocrinol. 1, 388–96.CrossRefGoogle ScholarPubMed
Hoppe, P. (1985). Technique of fertilization in vitro. In Reproductive Toxicology, (ed. Dixon, R.), pp. 191–9. New York: Raven Press.Google Scholar
Kobayashi, M., Hirako, M., Minato, Y., Sasaki, K., Horiuchi, R. & Domeki, I. (1996). Rat hepatoma Reuber H-35 cells produce a 2-cell stage specific inhibitor of the cleavage of mouse embryos. Biol. Reprod. 54, 364–70.CrossRefGoogle ScholarPubMed
Kobayashi, M., Hirako, M., Minato, Y., Sasaki, K., Horiuchi, R. & Domeki, I. (1997). Rat hepatoma Reuber H-35 cells produce factors that promote the hatching of mouse embryos cultured in vitro. Biol. Reprod. 56, 1041–9.CrossRefGoogle ScholarPubMed
Lu, R.Z., Shiota, K., Toyoda, Y. & Takahashi, M. (1990). Activin A (EDF) releases the “two-cell block” of mouse embryos in culture. Jpn. J. Anim. Reprod. 36, 127–32.CrossRefGoogle Scholar
Lu, R., Matsuyama, S., Nishihara, M. & Takahashi, M. (1993). Developmental expression of activin/inhibin βA, βB and α subunits and activin receptor-IIB genes in preimplantation mouse embryos. Biol. Reprod. 49, 1163–9.CrossRefGoogle Scholar
Lu, R.Z., Ikeda, A. & Takahashi, M. (1994). Activin A and superoxide dismutase synergistically enhance development of 1-cell mouse embryos to the blastocyst stage in vitro. J. Reprod. Dev. 40, 279–84.CrossRefGoogle Scholar
Marquant-Le Guienne, B., Gerard, M., Solari, A. & Thibault, C. (1989). In vitro culture of bovine egg fertilized either in vivo or in vitro. Reprod. Nutr Dev. 29, 559–68.CrossRefGoogle ScholarPubMed
Massague, J., Kelly, B. & Mottola, C. (1985). Stimulation by insulin-like growth factors is required for cellular transformation by type β transforming growth factor. J. Biol. Chem. 260, 4551–4.CrossRefGoogle ScholarPubMed
Minami, N., Utsumi, K. & Iritani, A. (1992). Effects of low molecular weight oviductal factors on the development of mouse one-cell embryos in vitro. J. Reprod. Fertil. 96, 735–45.CrossRefGoogle ScholarPubMed
Myers, M.W., Broussard, J.R., Menezo, Y., Prough, S.G., Blackwell, J., Godke, R.A. & Thibodeaux, J.K. (1994). Established cell lines and their conditioned media support bovine embryo development during in-vitro culture. Human Reprod. 9, 1927–31.CrossRefGoogle ScholarPubMed
Ogawa, T. & Marrs, R.P. (1987). The effect of protein supplementation on single-cell mouse embryos in vitro. Fertil. Steril. 47, 156–61.CrossRefGoogle ScholarPubMed
Ogawa, T., Ono, T. & Marrs, R.P. (1987). The effect of serum fractions on single-cell mouse embryos in vitro. J. In Vitro Fertil. Embryo Transfer 4, 153–8.CrossRefGoogle ScholarPubMed
Pampfer, S., Arceci, R.J. & Pollard, J.W. (1991). Role of colony stimulating factor-I (CSF-I) and lymphohematopoietic growth factors in mouse preimplantation development. Bioessays 13, 535–40.CrossRefGoogle Scholar
Quinn, P., Barros, C. & Whittingham, D.G. (1982). Preservation of hamster oocytes to assay the fertilizing capacity of human spermatozoa. J. Reprod. Fertil. 66, 161–8.CrossRefGoogle ScholarPubMed
Rappolee, D.A., Brenner, C.A., Schultz, R., Mark, D. & Werb, Z. (1988). Developmental expression of PDGF, TGF-α and TGF-β genes in preimplantation mouse embryos. Science 241, 1823–5.CrossRefGoogle ScholarPubMed
Reed, W.A., Tae-kwang, S., Bunch, T.D. & White, K.L. (1996). Culture of in vitro fertilized bovine embryos with bovine oviductal epithelial cells, buffalo rat liver (BRL) cells, or BRL-cell-conditioned medium. Theriogenology 45, 439–49.CrossRefGoogle ScholarPubMed
Rehman, N., Collins, A.R., Suh, T.K. & Wright, R.W.J. (1994). Development of in vitro matured and fertilized bovine oocytes co-cultured with buffalo rat liver cells. Theriogenology 41, 1453–62.CrossRefGoogle ScholarPubMed
Saito, H., Berger, T., Mishell, D.R. & Marrs, R.P. (1984). The effect of serum fractions on embryo growth. Fertil. Steril. 41, 761–5.CrossRefGoogle ScholarPubMed
Sakkas, D. & Trounson, A.O. (1990). Co-culture of mouse embryos with oviduct and uterine cells prepared from mice at different days of pseudopregnancy. J. Reprod. Fertil. 90, 109–18.CrossRefGoogle ScholarPubMed
Sharkey, A.M., Dellow, K., Blayney, M., Macnamee, M., Charnock-Jones, S. & Smith, S.K. (1995). Stage-specific expression of cytokine and receptor messenger ribonucleic acids in human preimplantation embryos. Biol. Reprod. 53, 955–62.CrossRefGoogle ScholarPubMed
Taniguchi, F., Harada, T., Nara, M., Deura, I., Mitsunari, M. & Terakawa, N. (2004). Coculture with a human granulosa cell line enhanced the development of murine preimplantation embryos via SCF/c-kit system. J. Assist. Reprod. Genet. 21, 223–8.CrossRefGoogle ScholarPubMed
Werb, Z. (1990). Expression of EGF and TGF-α genes in early mammalian development. Mol. Reprod. Dev. 27, 10–15.CrossRefGoogle ScholarPubMed
Yoneda, A., Okada, A., Wakayama, T., Ueda, J. & Watanabe, T. (2006). Analysis of specific factors generating 2-cell block in AKR mouse embryos. Zygote 14, 169–79.CrossRefGoogle ScholarPubMed
Yoshioka, K. & Kamomae, H. (1996). Recombinant human activin A stimulates development of bovine one-cell embryos matured and fertilized in vitro. Mol. Reprod. Dev. 45, 151–6.3.0.CO;2-S>CrossRefGoogle ScholarPubMed
Yoshioka, K., Suzuki, C. & Iwamura, S. (1998). Activin A and follistatin regulate developmental competence of in vitro-produced bovine embryos. Biol. Reprod. 59, 1017–22.CrossRefGoogle ScholarPubMed
Yoshioka, K., Suzuki, C. & Iwamura, S. (2000). Effects of activin A and follistatin on developmental kinetics of bovine embryos: cinematographic analysis in a chemically defined medium. J. Reprod. Fertil. 118, 119–25.CrossRefGoogle Scholar
Zsebo, K.M., Wypych, J., McNiece, I.K., Lu, H.S., Smith, K.A., Karkare, S.B., Sachdev, R.S., Yuschenkoff, V.N., Birkett, N.C., Williams, L.R. et al. (1990). Identification, purification and biological characterization of hematopoietic stem cell factor from buffalo rat liver-conditioned medium. Cell 63, 195–201.CrossRefGoogle ScholarPubMed