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Effect of exogenous transforming growth factor β1 (TGF-β1) on early bovine embryo development

Published online by Cambridge University Press:  08 June 2018

Antonio D. Barrera Open the ORCID record for Antonio D. Barrera [Opens in a new window] ,
Elina V. García  and
Dora C. Miceli
Antonio D. Barrera*
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología ‘Dr. Francisco D. Barbieri,’ Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina
Elina V. García
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología ‘Dr. Francisco D. Barbieri,’ Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina.
Dora C. Miceli
Affiliation:
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología ‘Dr. Francisco D. Barbieri,’ Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina.
*
All correspondence to: Antonio Daniel Barrera. Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología ‘Dr. Francisco D. Barbieri,’ Facultad de Bioquímica, Química y Farmacia, UNT. Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina. Telephone: +54 381 4247752, ext. 7099. Fax: +54 381 4248921. E-mail: [email protected]
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Summary

During preimplantation development, embryos are exposed and have the capacity to respond to different growth factors present in the maternal environment. Among these factors, transforming growth factor β1 (TGF-β1) is a well known modulator of embryonic growth and development. However, its action during the first stages of development, when the embryo transits through the oviduct, has not been yet elucidated. The objective of the present study was to examine the effect of early exposure to exogenous TGF-β1 on embryo development and expression of pluripotency (OCT4, NANOG) and DNA methylation (DNMT1, DNMT3A, DNMT3B) genes in bovine embryos produced in vitro. First, gene expression analysis of TGF-β receptors confirmed a stage-specific expression pattern, showing greater mRNA abundance of TGFBR1 and TGFBR2 from the 2- to the 8-cell stage, before embryonic genome activation. Second, embryo culture for the first 48 h in serum-free CR1aa medium supplemented with 50 or 100 ng/ml recombinant TGF-β1 did not affect the cleavage and blastocyst rate (days 7 and 8). However, RT-qPCR analysis showed a significant increase in the relative abundance of NANOG and DNMT3A in the 8-cell stage embryos and expanded blastocysts (day 8) derived from TGF-β1 treated embryos. These results suggest an early action of exogenous TGF-β1 on the bovine embryo, highlighting the importance to provide a more comprehensive understanding of the role of TGF-β signalling during early embryogenesis.

Keywords

Early developmentDNA methylationGrowth factorsOviductPluripotency
Type
Research Article
Information
Zygote , Volume 26 , Issue 3 , June 2018 , pp. 232 - 241
Copyright
Copyright © Cambridge University Press 2018 

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