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Distribution pattern and activity of mitochondria during oocyte growth and maturation in the ascidian Styela plicata*

Published online by Cambridge University Press:  18 January 2013

Amina Bezzaouia ,
Alessandra Gallo ,
Francesco Silvestre ,
Saïda Tekaya  and
Elisabetta Tosti
Amina Bezzaouia
Affiliation:
Université de Tunis El-Manar, Faculté des Sciences de Tunis, UR11ES12 Biologie de la Reproduction et du Développement Animal, 2092, Tunis, Tunisie
Alessandra Gallo
Affiliation:
Animal Physiology and Evolution Laboratory, Stazione Zoologica Anton Dohrn, Napoli, Italy.
Francesco Silvestre
Affiliation:
Animal Physiology and Evolution Laboratory, Stazione Zoologica Anton Dohrn, Napoli, Italy.
Saïda Tekaya
Affiliation:
Université de Tunis El-Manar, Faculté des Sciences de Tunis, UR11ES12 Biologie de la Reproduction et du Développement Animal, 2092, Tunis, Tunisie
Elisabetta Tosti*
Affiliation:
Animal Physiology and Evolution Laboratory, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy.
*
All correspondence to: Elisabetta Tosti. Animal Physiology and Evolution Laboratory, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, Italy. Tel: +39 0815833288. Fax: +39 0817641355. e-mail: [email protected]
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Summary

The process of oocyte maturation is underlined by a redistribution of cellular organelles, among which mitochondria play a functional role for the acquisition of fertilization and developmental competence. In this paper, we applied electron and confocal microscopy by using DIOC6 and JC-1 stain to evaluate mitochondria distribution pattern and activity during different stages of oocyte growth in the ascidian Styela plicata. Three categories of oocytes at the germinal vesicle stage underlying the vitellogenic process were characterized on the basis of size, pigmentation and accessory cells. Mitochondria were spread throughout the cytoplasm at the smallest oocyte stage and gradually migrated to the periphery of the subcortical cytoplasm at the intermediate stage. At the fully grown oocyte stage, mitochondria were aggregated in the subcortical cytoplasm. This pattern of polarized mitochondria distribution correlates significantly with an increase in mitochondria potential and activity. In this paper we discuss the relationship of mitochondria to the acquisition of oocyte developmental competence.

Keywords

Mitochondria distributionMitochondria membrane potentialOocyte growthOocyte maturationStyela plicata
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 462 - 469
Copyright
Copyright © Cambridge University Press 2013 

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Footnotes

*

This work is dedicated to the dear memory of Prof. Charles Lambert.

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