Hostname: page-component-cd9895bd7-gbm5v Total loading time: 0 Render date: 2024-12-27T01:55:37.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Differential expression of microRNAs in 2-cell and 4-cell mouse embryos

Published online by Cambridge University Press:  26 April 2013

Pei Wang ,
Ji Cui ,
Chun Zhao ,
Lin Zhou ,
Xirong Guo ,
Rong Shen ,
Junqiang Zhang  and
Xiufeng Ling
Pei Wang
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Ji Cui
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Chun Zhao
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Lin Zhou
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Xirong Guo
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Rong Shen
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210004, China.
Junqiang Zhang*
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China.
Xiufeng Ling*
Affiliation:
State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China.
*
All correspondence to: Xiufeng Ling or to Junqiang Zhang. State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China. Tel:/Fax: +86 25 52226248. e-mail: [email protected] or [email protected].
All correspondence to: Xiufeng Ling or to Junqiang Zhang. State Key Laboratory of Reproductive Medicine, Department of Reproduction, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing Medical University, Nanjing, Jiangsu Province, China. Tel:/Fax: +86 25 52226248. e-mail: [email protected] or [email protected].
Get access Rights & Permissions [Opens in a new window]

Summary

In vitro fertilized (IVF) human embryos have a high incidence of developmental arrest before the blastocyst stage, therefore characterization of the molecular mechanisms that regulate embryo development is urgently required. Post-transcriptional control by microRNAs (miRNAs) is one of the most investigated RNA control mechanisms, and is hypothesized to be involved actively in developmental arrest in preimplantation embryos. In this study, we extracted total RNA from mouse 2-cell and 4-cell embryos. Using a miRNA microarray, 192 miRNAs were found to be differentially expressed in 4-cell embryos and 2-cell embryos; 122 miRNAs were upregulated and 70 were downregulated in 4-cell embryos. The microarray results were confirmed by real-time quantitative RT-PCR for six miRNAs (mmu-miR-467h, mmu-miR-466d-3p, mmu-miR-292–5p, mmu-miR-154, mmu-miR-2145, and mmu-miR-706). Cdca4 and Tcf12 were identified as miR-154 target genes by target prediction analysis. This study provides a developmental map for a large number of miRNAs in 2-cell and 4-cell embryos. The function of these miRNAs and the mechanisms by which they modulate embryonic developmental arrest require further study. The results of this study have potential applications in the field of reproductive medicine.

Keywords

2-cell embryo4-cell embryoDevelopmental arrestmiRNA
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 455 - 461
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abbott, A.L., Alvarez-Saavedra, E., Miska, E.A., Lau, N.C., Bartel, D.P., Horvitz, H.R. & Ambros, V. (2005). The let-7 microRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegans. Dev. Cell. 9, 403–14.Google Scholar
Abrams, E.W. & Mullins, M.C. (2009). zebrafish development: it's in the maternal genes. Curr. Opin. Genet. Dev. 19, 396–403.Google Scholar
Alizadeh, Z., Kageyama, S. & Aoki, F. (2005). Degradation of maternal mRNA in mouse embryos: selective degradation of specific mRNAs after fertilization. Mol. Reprod. Dev. 72, 281–90.Google Scholar
Bartel, D.P. (2004). MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281–97.Google Scholar
Bashirullah, A., Halsell, S.R., Cooperstock, R.L., Kloc, M., Karaiskakis, A., Fisher, W.W., Fu, W., Hamilton, J.K., Etkin, L.D. & Lipshitz, H.D. (1999). Joint action of two RNA degradation pathways controls the timing of maternal transcript elimination at the midblastula transition in Drosophila melanogaster. EMBO J. 18, 2610–20.Google Scholar
Bettegowda, A., Lee, K.B. & Smith, G.W. (2008). Cytoplasmic and nuclear determinants of the maternal-to-embryonic transition. Reprod. Fertil. Dev. 20, 45–53.Google Scholar
Bushati, N. & Cohen, S.M. (2007). MicroRNA functions. Annu. Rev. Cell Dev. Biol. 23, 175–205.Google Scholar
Bushati, N., Stark, A., Brennecke, J. & Cohen, S.M. (2008). Temporal reciprocity of miRNAs and their targets during the maternal-to-zygotic transition in Drosophila. Curr. Biol. 18, 501–6.Google Scholar
Cui, X.S., Li, X.Y., Jeong, Y.J., Jun, J.H. & Kim, N.H. (2006). Gene expression of cox5a, 5b, or 6b1 and their roles in preimplantation mouse embryos. Biol. Reprod. 74, 601–10.Google Scholar
De Renzis, S., Elemento, O., Tavazoie, S. & Wieschaus, E.F. (2007). Unmasking activation of the zygotic genome using chromosomal deletions in the Drosophila embryo. PLoS Biol. 5, e117.Google Scholar
Delić, D., Dkhil, M., Al-Quraishy, S. & Wunderlich, F. (2011). Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria. Parasitol. Res. 108, 1111–21.Google Scholar
Farley, B.M. & Ryder, S.P. (2008). Regulation of maternal mRNAs in early development. Crit. Rev. Biochem. Mol. Biol. 43, 135–62.CrossRefGoogle ScholarPubMed
Giraldez, A.J., Cinalli, R.M., Glasner, M.E., Enright, A.J., Thomson, J.M., Baskerville, S., Hammond, S.M., Bartel, D.P. & Schier, A.F. (2005). MicroRNAs regulate brain morphogenesis in zebrafish. Science 308, 833–8.Google Scholar
Giraldez, A.J., Mishima, Y., Rihel, J., Grocock, R.J., Van Dongen, S., Inoue, K., Enright, A.J. & Schier, A.F. (2006). Zebrafish miR-430 promotes deadenylation and clearance of maternal mRNAs. Science 312, 75–9.Google Scholar
Hamatani, T., Carter, M.G., Sharov, A.A. & Ko, M.S. (2004). Dynamics of global gene expression changes during mouse preimplantation development. Dev. Cell 6, 117–31.Google Scholar
Chen, Hong, Qian, Kun, Ping Tang, Zhou, Xing, Bianzhi, Chen, Haiping, Liu, Na, Huang, Xiaolin & Zhang, Suming. (2010). Bioinformatics and microarray analysis of microRNA expression profiles of murine embryonic stem cells, neural stem cells induced from ESCs and isolated from E8.5 mouse neural tube. Neurol. Res. 32, 603–13.Google Scholar
Houbaviy, H.B., Murray, M.F. & Sharp, P.A. (2003). Embryonic stem cell-specific microRNA. Dev. Cell 5, 351–8.Google Scholar
Zhang, Junqiang, Cui, Ji, Ling, Xiufeng, Xiuling, Li, Peng, Yuzhu, Xirong, Guo, Chin Heng, Boon & Tong, Guo Qing. (2009). Vitrification of mouse embryos at 2-cell, 4-cell and 8-cell stages by cryotop method. J. Assist. Reprod. Genet. 26 (11–12), 621–8.Google Scholar
Lawitts, J.A. & Biggers, J.D. (1991). Overcoming the 2-cell block by modifying standard components in a mouse embryo culture medium. Biol. Reprod. 45, 245–51.Google Scholar
Lee, R.C., Feinbaum, R.L. & Ambros, V. (1993). The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75, 843–54.Google Scholar
Lian, H., Liu, W., Liu, Q., Jin, H., Sun, Y., Li, J., Xia, Z. & Gao, H. (2010). A laboratory-attenuated vesicular stomatitis virus induces apoptosis and alters the cellular microRNA expression profile in BHK cells. Arch. Virol. 155, 1643–53.Google Scholar
Lund, E., Liu, M., Hartley, R.S., Sheets, M.D. & Dahlberg, J.E. (2009). Deadenylation of maternal mRNAs mediated by miR-427 in Xenopus laevis embryos. RNA 15, 2351–63.Google Scholar
Redell, J.B., Liu, Y. & Dash, P.K. (2009). Traumatic brain injury alters expression of hippocampal microRNAs: potential regulators of multiple patho-physiological processes. J. Neurosci. Res. 87, 1435–48.CrossRefGoogle Scholar
Reinhart, B.J., Slack, F.J., Basson, M., Pasquinelli, A.E., Bettinger, J.C., Rougvie, A.E., Horvitz, H.R. & Ruvkun, G. (2000). The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature 403, 901–6.Google Scholar
Rosa, A., Spagnoli, F.M. & Brivanlou, A.H. (2009). The miR-430/427/302 family controls mesendodermal fate specification via species-specific target selection. Dev. Cell 16, 517–27.Google Scholar
Shkumatava, A., Stark, A., Sive, H. & Bartel, D.P. (2009). Coherent but over lapping expression of microRNAs and their targets during vertebrate development. Genes Dev. 23, 466–81.Google Scholar
Su, N., Wang, Y., Qian, M. & Deng, M. (2010). Combinatorial regulation of transcription factors and microRNAs. BMC Syst. Biol. 4, 150.CrossRefGoogle ScholarPubMed
Tadros, W. & Lipshitz, H.D. (2009). The maternal-to-zygotic transition: a play in two acts. Development 136, 3033–42.Google Scholar
Takada, S., Berezikov, E., Yamashita, Y., Lagos-Quintana, M., Kloosterman, W.P., Enomoto, M., Hatanaka, H., Fujiwara, S., Watanabe, H., Soda, M., Choi, Y.L., Plasterk, R.H., Cuppen, E. & Mano, H. (2006). Mouse microRNA profiles determined with a new and sensitive cloning method. Nucl. Acids Res. 34, e115.Google Scholar
Tang, F., Kaneda, M., O'Carroll, D., Hajkova, P., Barton, S.C., Sun, Y.A., Lee, C., Tarakhovsky, A., Lao, K. & Surani, M.A. (2007). Maternal microRNAs are essential for mouse zygotic development. Genes Dev. 21, 644–8.Google Scholar
Wang, L.L., Zhang, Z., Li, Q., Yang, R., Pei, X., Xu, Y., Wang, J., Zhou, S.F. & Li, Y. (2009). Ethanol exposure induces differential microRNA and target gene expression and teratogenic effects which can be suppressed by folic acid supplementation. Hum. Reprod. 24, 562–79.Google Scholar
Watanabe, T., Imai, H. & Minami, N. (2008). Identification and expression analysis of small RNAs during development. Methods Mol. Biol. 442, 173–85.Google Scholar
Wu, L., Fan, J. & Belasco, J.G. (2006). MicroRNAs direct rapid deadenylation of mRNA. Proc. Natl. Acad. Sci. USA 103, 4034–9.CrossRefGoogle ScholarPubMed
Ling, Xiu Feng, Zhang, Jun Qiang, Cao, Shan Ren, Chen, Jie, Peng, Yuzhu, Guo, Xirong, Heng, Boon Chin, Tong, Guo Qing & Wang, Xinru. (2009). Effect of cryotop vitrification on preimplantation developmental competence of murine morula and blastocyst stage embryos. Reprod. BioMed. Online 19, 708–13.Google Scholar
Zeng, F., Baldwin, D.A. & Schultz, R.M. (2004). Transcript profiling during preimplantation mouse development. Dev. Biol. 272, 483–96.CrossRefGoogle ScholarPubMed