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Decrease in fertilization and cleavage rates, but not in clinical outcomes for infertile men with AZF microdeletion of the Y chromosome

Published online by Cambridge University Press:  15 October 2014

Yuan-Chang Zhu ,
Tong-Hua Wu ,
Guan-Gui Li ,
Biao Yin ,
Hong-Jie Liu ,
Cheng Song ,
Mei-Lan Mo  and
Yong Zeng
Yuan-Chang Zhu
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Tong-Hua Wu
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Guan-Gui Li
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Biao Yin
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Hong-Jie Liu
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Cheng Song
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Mei-Lan Mo
Affiliation:
Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Fertility Center, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PRChina. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
Yong Zeng*
Affiliation:
Fertility Center, Zhongshan Urology Hospital, Shenzhen 518048, China Shenzhen Key Laboratory of Reproductive Immunology for Peri-implantation, Shenzhen Zhongshan Urology Hospital, Shenzhen 518045, PR China. Shenzhen Zhongshan Institute for Reproduction and Genetics, Shenzhen 518045, PRChina.
*
All correspondence to: Yong Zeng. Fertility Center, Zhongshan Urology Hospital, Shenzhen 518048, China. Tel: +86 755 83381691. Fax: +86 755 83381691-8001. e-mail: [email protected].
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Summary

This study aimed to explore whether the presence of a Y chromosome azoospermia factor (AZF) microdeletion confers any adverse effect on embryonic development and clinical outcomes after intracytoplasmic sperm injection (ICSI) treatment. Fifty-seven patients with AZF microdeletion were included in the present study and 114 oligozoospermia and azoospermia patients without AZF microdeletion were recruited as controls. Both AZF and control groups were further divided into subgroups based upon the methods of semen collection: the AZF-testicular sperm extraction subgroup (AZF-TESE, n = 14), the AZF-ejaculation subgroup (AZF-EJA, n = 43), the control-TESE subgroup (n = 28) and the control-EJA subgroup (n = 86). Clinical data were analyzed in the two groups and four subgroups respectively. A retrospective case–control study was performed. A significantly lower fertilization rate (69.27 versus 75.70%, P = 0.000) and cleavage rate (89.55 versus 94.39%, P = 0.000) was found in AZF group compared with the control group. Furthermore, in AZF-TESE subgroup, the fertilization rate (67.54 versus 74.25%, P = 0.037) and cleavage rate (88.96 versus 94.79%, P = 0.022) were significantly lower than in the control-TESE subgroup; similarly, the fertilization rate (69.85 versus 75.85%, P = 0.004) and cleavage rate (89.36 versus 94.26%, P = 0.002) in AZF-EJA subgroup were significantly lower than in the control-EJA subgroup; however, the fertilization rate and cleavage rate in AZF-TESE (control-TESE) subgroup was similar to that in the AZF-EJA (control-EJA) subgroup. The other clinical outcomes were comparable between four subgroups (P > 0.05). Therefore, sperm from patients with AZF microdeletion, obtained either by ejaculation or TESE, may have lower fertilization and cleavage rates, but seem to have comparable clinical outcomes to those from patients without AZF microdeletion.

Keywords

AZF microdeletionClinical outcomeCleavage rateEmbryonic developmentFertilization rate
Type
Research Article
Information
Zygote , Volume 23 , Issue 5 , October 2015 , pp. 771 - 777
Copyright
Copyright © Cambridge University Press 2014 

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References

Aitken, R.J. & De Iuliis, G.N. (2010). On the possible origins of DNA damage in human spermatozoa. Mol. Hum. Reprod. 16, 3–13.CrossRefGoogle ScholarPubMed
Bellver, J., Meseguer, M., Muriel, L., Garcia-Herrero, S., Barreto, M.A., Garda, A.L., Remohi, J., Pellicer, A. & Garrido, N. (2010). Y chromosome microdeletions, sperm DNA fragmentation and sperm oxidative stress as causes of recurrent spontaneous abortion of unknown etiology. Hum. Reprod. 25, 1713–21.CrossRefGoogle ScholarPubMed
Choi, J.M., Chung, P., Veeck, L., Mielnik, A., Palermo, G.D. & Schlegel, P.N. (2004). AZF microdeletions of the Y chromosome and in vitro fertilization outcome. Fertil. Steril. 81, 337–41.CrossRefGoogle Scholar
Dai, R.L., Wang, R.X., Jin, J.L., Niu, G.N., Lee, J.Y., Li, S.B. & Liu, R.Z. (2012). Correlation of Y-chromosome multiple segmental deletions and chromosomal anomalies in non-obstructive azoospermic males from northeastern China. Genet. Mol. Res. 11, 2422–31.CrossRefGoogle ScholarPubMed
Dewan, S., Puscheck, E.E., Coulam, C.B., Wilcox, A.J. & Jeyendran, R.S. (2006). Y-chromosome microdeletions and recurrent pregnancy loss. Fertil. Steril. 85, 441–5.CrossRefGoogle ScholarPubMed
Dohle, G.R., Halley, D.J., Van Hemel, J.O., van den Ouwel, A.M., Pieters, M.H., Weber, R.F. & Govaerts, L.C. (2002). Genetic risk factors in infertile men with severe oligozoospermia and azoospermia. Hum. Reprod. 17, 13–6.CrossRefGoogle ScholarPubMed
Figueira Rde, C., Braga, D.P., Setti, A.S., Iaconelli, A. Jr. & Borges, E. Jr. (2011). Morphological nuclear integrity of sperm cells is associated with preimplantation genetic aneuploidy screening cycle outcomes. Fertil. Steril. 95, 990–3.CrossRefGoogle ScholarPubMed
Ghorbel, M., Gargouri Baklouti, S., Ben Abdallah, F., Zribi, N., Cherif, M., Keskes, R., Chakroun, N., Sellami, A., Belguith, N., Kamoun, H., Fakhfakh, F. & Ammar-Keskes, L. (2012). Chromosomal defects in infertile men with poor semen quality. J. Assist. Reprod. Genet. 29, 451–6.CrossRefGoogle ScholarPubMed
Karaer, A., Karaer, K., Ozaksit, G., Ceylaner, S. & Percin, E.F. (2008). Y chromosome azoospermia factor region microdeletions and recurrent pregnancy loss. Am. J. Obstet. Gynecol. 199, 662 e661–5.CrossRefGoogle ScholarPubMed
Khan, F.H., Ganesan, P. & Kumar, S. (2010). Y chromosome microdeletion and altered sperm quality in human males with high concentration of seminal hexachlorocyclohexane (HCH). Chemosphere 80, 972–7.CrossRefGoogle ScholarPubMed
Kihaile, P.E., Kisanga, R.E., Aoki, K., Kumasako, Y., Misumi, J. & Utsunomiya, T. (2004). Embryo outcome in Y-chromosome microdeleted infertile males after ICSI. Mol. Reprod. Dev. 68, 176–81.CrossRefGoogle ScholarPubMed
Liu, X.H., Qiao, J., Li, R., Yan, L.Y. & Chen, L.X. (2013). Y chromosome AZFc microdeletion may not affect the outcomes of ICSI for infertile males with fresh ejaculated sperm. J. Assist. Reprod. Genet. 30, 813–9.CrossRefGoogle Scholar
Martin, R.H. (2008). Cytogenetic determinants of male fertility. Human Reprod. Update 14, 379–90.CrossRefGoogle ScholarPubMed
Massart, A., Lissens, W., Tournaye, H. & Stouffs, K. (2012). Genetic causes of spermatogenic failure. Asian J. Androl. 14, 40–8.CrossRefGoogle ScholarPubMed
Monqaut, A.L., Zavaleta, C., Lopez, G., Lafuente, R. & Brassesco, M. (2011). Use of high-magnification microscopy for the assessment of sperm recovered after two different sperm processing methods. Fertil. Steril. 95, 277–80.CrossRefGoogle ScholarPubMed
Pandey, L.K., Pandey, S., Gupta, J. & Saxena, A.K. (2010). Loss of the AZFc region due to a human Y-chromosome microdeletion in infertile male patients. Genet. Mol. Res. 9, 1267–73.CrossRefGoogle ScholarPubMed
Pryor, J.L., Kent-First, M., Muallem, A., Van Bergen, A.H., Nolten, W.E., Meisner, L. & Roberts, K.P. (1997). Microdeletions in the Y chromosome of infertile men. New Engl. J. Med. 336, 534–9.CrossRefGoogle Scholar
Rossetto, R., Saraiva, M.V., dos Santos, R.R., da Silva, C.M., Faustino, L.R., Chaves, R.N., Brito, I.R., Rodrigues, G.Q., Lima, I.M., Donato, M.A., Peixoto, C.A. & de Figueiredo, J.R. (2013). Effect of medium composition on the in vitro culture of bovine pre-antral follicles: morphology and viability do not guarantee functionality. Zygote 21, 125–8.CrossRefGoogle Scholar
Scott, L., Alvero, R., Leondires, M. & Miller, B. (2000). The morphology of human pronuclear embryos is positively related to blastocyst development and implantation. Hum. Reprod., 15, 2394–03.CrossRefGoogle ScholarPubMed
Silber, S.J. (2000). Microsurgical TESE and the distribution of spermatogenesis in non-obstructive azoospermia. Hum. Reprod., 15, 2278–84.CrossRefGoogle ScholarPubMed
Simon, L., Brunborg, G., Stevenson, M., Lutton, D., McManus, J. & Lewis, S.E. (2010). Clinical significance of sperm DNA damage in assisted reproduction outcome. Human Reprod. 25, 1594–08.CrossRefGoogle ScholarPubMed
Souza Setti, A., Ferreira, R.C., Paes de Almeida Ferreira Braga, D., de Cassia Savio Figueira, R., Iaconelli, A. Jr. & Borges, E. Jr. (2010). Intracytoplasmic sperm injection outcome versus intracytoplasmic morphologically selected sperm injection outcome: a meta-analysis. Reprod. Biomed. Online, 21, 450–5.CrossRefGoogle ScholarPubMed
Tsai, C.C., Huang, F.J., Wang, L.J., Lin, Y.J., Kung, F.T., Hsieh, C.H. & Lan, K.C. (2011). Clinical outcomes and development of children born after intracytoplasmic sperm injection (ICSI) using extracted testicular sperm or ejaculated extreme severe oligo-astheno-teratozoospermia sperm: a comparative study. Fertil. Steril. 96, 567–71.CrossRefGoogle ScholarPubMed
van Golde, R.J., Wetzels, A.M., de Graaf, R., Tuerlings, J.H., Braat, D.D. & Kremer, J.A. (2001). Decreased fertilization rate and embryo quality after ICSI in oligozoospermic men with microdeletions in the azoospermia factor c region of the Y chromosome. Hum. Reprod, 16, 289–92.CrossRefGoogle ScholarPubMed
Visser, L. & Repping, S. (2010). Unravelling the genetics of spermatogenic failure. Reproduction 139, 303–7.CrossRefGoogle ScholarPubMed
Wettasinghe, T.K., Jayasekara, R.W. & Dissanayake, V.H. (2010). Y chromosome microdeletions are not associated with spontaneous recurrent pregnancy loss in a Sinhalese population in Sri Lanka. Hum. Reprod. 25, 3152–6.CrossRefGoogle ScholarPubMed
World Health Organization (1999). WHO Laboratory Manual for the Examination of Human Semen and Sperm–Cervical Mucus Interaction. Cambridge: Cambridge University Press.Google Scholar
Yazawa, H., Yanagida, K. & Sato, A. (2007). Human round spermatids from azoospermic men exhibit oocyte-activation and Ca2+ oscillation-inducing activities. Zygote 15, 337–46.CrossRefGoogle ScholarPubMed
Yu, S., Koong, M., Seo, J., Lee, H., Cha, S. & Yang, K. (2006). P-603: Effects of Y chromosome microdeletion on in vitro fertilization outcome. Fertil. Steril. 86, S357–8.CrossRefGoogle Scholar
Zhang, F., Li, L., Wang, L., Yang, L., Liang, Z., Li, J., Jin, F. & Tian, Y. (2013). Clinical characteristics and treatment of azoospermia and severe oligospermia patients with Y-chromosome microdeletions. Mol. Reprod. Dev. 80, 908–15.CrossRefGoogle ScholarPubMed