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Connexin37 mRNA expression in in vivo and in vitro mouse oocyte

Published online by Cambridge University Press:  01 May 2009

Bao Ying Yin ,
Yong Zhang ,
Jian Hong Sun ,
Ji Xia Li  and
Ye Fei Ma
Bao Ying Yin
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Yong Zhang*
Affiliation:
College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China.
Jian Hong Sun
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Ji Xia Li
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
Ye Fei Ma
Affiliation:
College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, People's Republic of China.
*
All correspondence to Yong Zhang. College of Veterinary Medicine, Northwest A & F University, Yangling, Shaanxi Province 712100, People's Republic of China. Tel: +86 29 87080085. Fax: +86 29 87080085. e-mail: [email protected]
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Summary

To evaluate gene expression of Connexin37 (Cx37) in oocytes from in vitro follicles at different stages, mouse preantral follicles were isolated and cultured for 12 days in vitro. Compared with in vitro follicles, follicles grown in vivo were collected at day 14 (d14), d16, d18, d20, d22 and d24 with the same stages for gene expression of Cx37 in oocytes. Our results showed that Cx37 mRNA increased along with follicular development, reached the highest level at the onset of antrum cavity formation and decreased after antrum formation in both in vivo and in vitro mouse oocytes. However, Cx37 mRNA was significant higher (p < 0.01) in in vitro cultured oocytes than in vivo oocytes. Moreover, significantly higher levels of Cx37 mRNA were found in oocytes from in vitro disrupted follicles (p < 0.01) and non-grown follicles (p < 0.05) than those from normal follicles with a similar size. These data determine temporal gene expression of Cx37 in oocytes from follicules at different stages and indicate that the gene expression level of Cx37 in oocytes could be evaluated as a criterion to the regulatory mechanism of Cx37 in an in vitro model.

Keywords

ConnexinFollicle cultureMouseOocyte development
Type
Research Article
Information
Zygote , Volume 17 , Issue 2 , May 2009 , pp. 163 - 168
Copyright
Copyright © Cambridge University Press 2009

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References

Baluska, F., Volkmann, D. & Barlow, P.W. (2004). Eukaryotic cells and their cell bodies: cell theory revised. Ann. Bot. 94, 9–32.CrossRefGoogle ScholarPubMed
Brower, P.T. & Schultz, R.M. (1982). Intercellular communication between granulosa cells and mouse oocytes: existence and possible nutritional role during oocyte growth. Dev. Biol. 90, 144–53.CrossRefGoogle ScholarPubMed
Carabatsos, M.J., Sellitto, C., Goodenough, D.A. & Albertini, D.F. (2000). Oocyte-granulosa cell heterologous gap junctions are required for the coordination of nuclear and cytoplasmic meiotic competence. Dev. Biol. 226, 167–79.CrossRefGoogle ScholarPubMed
Demeestere, I., Centner, J., Gervy, C., Englert, Y. & Delbaere, A. (2005). Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents. Reproduction 130, 147–56.CrossRefGoogle ScholarPubMed
Diaz, F.J., Wigglesworth, K. & Eppig, J.J. (2007). Oocytes determine cumulus cell lineage in mouse ovarian follicles. Cell. Sci. 120, 1330–40.CrossRefGoogle ScholarPubMed
Drummond, A.E. (2006). The role of steroids in follicular growth. Reprod. Biol. Endocrinol. 4, 16.CrossRefGoogle ScholarPubMed
Eppig, J.J. (1991). Intercommunication between mammalian oocytes and companion somatic cells. Bio. Essays 13, 569–74.Google ScholarPubMed
Eppig, J. (2005). Mouse oocytes control metabolic co-operativity between oocytes and cumulus cells. Reprod. Fertil. Dev. 17, 1–2.CrossRefGoogle ScholarPubMed
Eppig, J.J. & O'Brien, M.J. (1996). Development in vitro of mouse oocytes from primordial follicles. Biol. Reprod. 54, 197–207.CrossRefGoogle ScholarPubMed
Folkman, J. & Moscona, A. (1978). Role of cell shape in growth control. Nature 73, 345–7.CrossRefGoogle Scholar
Gilchrist, R.B., Ritter, L.J., Myllymaa, S., Kaivo-Oja, N., Dragovic, R.A., Hickey, T.E., Ritvos, O. & Gittens, J.E. & Kidder, G.M. (2005). Differential contributions of connexin37 and connexin43 to oogenesis revealed in chimeric reaggregated mouse ovaries. J. Cell. Sci. 118, 5071–8.Google Scholar
Goldberg, G.S., Valiunas, V. & Brink, P.R. (2004). Selective permeability of gap junction channels. Biochim. Biophys. Acta 1662, 96–101.CrossRefGoogle ScholarPubMed
Goodenough, D.A., Goliger, J.A. & Paul, D.L. (1996). Connexins, connexons and intercellular communication. Annu. Rev. Biochem. 65, 475–502.CrossRefGoogle ScholarPubMed
Granot, I. & Dekel, N. (1998). Cell-to-cell communication in the ovarian follicle: developmental and hormonal regulation of the expression of connexin43. Hum.. Reprod. 13, 85–97.CrossRefGoogle ScholarPubMed
Hammes, S.R. (2004). Steroids and oocyte maturation—a new look at an old story. Mol. Endocrinol. 18, 769–75.CrossRefGoogle Scholar
Heller, D.T., Cahill, D.M. & Schultz, R.M. (1981). Biochemical studies of mammalian oogenesis, metabolic cooperativity between granulosa cells and growing mouse oocytes. Dev. Biol. 84, 455–64.CrossRefGoogle ScholarPubMed
Herlands, R.L. & Schultz, R.M. (1984). Regulation of mouse oocyte growth; probable nutritional role for intercellular communication between follicle cells and oocytes in oocyte growth. J. Exp. Zool. 229, 317–25.CrossRefGoogle ScholarPubMed
Juneja, S.C., Barr, K.J., Enders, G.C. & Kidder, G.M. (1999). Defects in the germ line and gonads of mice lacking connexin43. Biol. Reprod. 60, 1263–70.CrossRefGoogle ScholarPubMed
Kidder, G.M. & Mhawi, A.A. (2002). Gap junctions and ovarian folliculogenesis. Reproduction 123, 613–20.CrossRefGoogle ScholarPubMed
Matzuk, M.M., Burns, K.H., Viveiros, M.M. & Eppig, J.J. (2002). Intercellular communication in the mammalian ovary: oocytes carry the conversation. Science 296, 2178–80.CrossRefGoogle ScholarPubMed
Neijssen, J., Herberts, C., Drijfhout, J.W., Reits, E., Janssen, L. & Neefjes, J. (2005). Cross-presentation by intercellular peptide transfer through gap junctions. Nature 434, 83–8.CrossRefGoogle ScholarPubMed
Nuttinck, F., Peynot, N., Humblot, P., Massip, A., Dessy, F. & Flechon, J.E. (2000). Comparative immunohistochemical distribution of connexin 37 and connexin 43 throughout folliculogenesis in the bovine ovary. Mol. Reprod. Dev. 57, 60–6.3.0.CO;2-6>CrossRefGoogle ScholarPubMed
O'Brien, M.J., Pendola, J.K. & Eppig, J.J. (2003). A revised protocol for in vitro development of mouse oocytes from primordial follicles dramatically improves their developmental competence. Biol. Reprod. 68, 1682–6.CrossRefGoogle ScholarPubMed
Shaw, R.M., Fay, A.J., Puthenveedu, M.A., von Zastrow, M., Jan, Y.N. & Jan, L.Y. (2007). Microtubule plus-end-tracking proteins target gap junctions directly from the cell interior to adherens junctions. Cell 128, 547–60.CrossRefGoogle ScholarPubMed
Simon, A.M., Chen, H. & Jackson, C.L. (2006). Cx37 and Cx43 localize to zona pellucida in mouse ovarian follicles. Cell. Commun. Adhes. 13, 61–77.CrossRefGoogle ScholarPubMed
Simon, A.M., Goodenough, D.A., Li, E. & Paul, D.L. (1997). Female infertility in mice lacking connexin 37. Nature 385, 525–9.CrossRefGoogle ScholarPubMed
Sternlicht, A.L. & Schultz, R.M. (1981). Biochemical studies of mammalian oogenesis, kinetics of accumulation of total and poly(A)-containing RNA during growth of the mouse oocyte. J. Exp. Zool. 215, 191–200.CrossRefGoogle ScholarPubMed
Sugiura, K. & Eppig, J.J. (2005). Control of metabolic cooperativity between oocytes and their companion granulosa cells by mouse oocytes. Reprod. Fertil. Dev. 17, 667–74.CrossRefGoogle ScholarPubMed
Tadvalkar, G. & Silva, P. (1983). In vitro, rapid assembly of gap junctions is induced by cytoskeleton disruptors. J. Cell. Biol. 96, 1279–87.CrossRefGoogle ScholarPubMed
Teilmann, S.C. (2005). Differential expression and localisation of connexin-37 and connexin-43 in follicles of different stages in the 4-week-old mouse ovary. Mol. Cell. Endocrinol. 234, 27–35.CrossRefGoogle ScholarPubMed
Veitch, G.I., Gittens, J.E., Shao, Q., Laird, D.W. & Kidder, G.M. (2004). Selective assembly of connexin37 into heterocellular gap junctions at the oocyte/granulosa cell interface. J. Cell. Sci. 117, 2699–707.CrossRefGoogle ScholarPubMed
Wassarman, P.M. & Albertini, D.F. (1994). The Mammalian Ovum. Physiology of Reproduction., pp. 79–122. New York: E. Knobil & J.D. Neill.Google Scholar
Wright, C.S., Becker, D.L., Lin, J.S., Warner, A.E. & Hardy, K. (2001). Stage-specific and differential expression of gap junctions in the mouse ovary: connexin-specific roles in follicular regulation. Reproduction 121, 77–88.CrossRefGoogle ScholarPubMed