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Blastocoele formation and cell allocation to the inner cell mass and trophectoderm in haploid and diploid pig parthenotes developing in vitro

Published online by Cambridge University Press:  26 September 2008

Nam-Hyung Kim ,
Sang Jun Uhm ,
Jin Young Ju ,
Hoon Taek Lee  and
Kil Saeng Chung
Nam-Hyung Kim
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Sang Jun Uhm
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Jin Young Ju
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Hoon Taek Lee
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
Kil Saeng Chung*
Affiliation:
Animal Resoures Research Center, Departmant of Animal Sciences, Kon-Kuk University, Seoul, Korea.
*
Kil Saeng Chung, Animal Resources Research Center, Department of Animal Sciences, Kon-Kuk University, Kwangjin-gu, Mojin-dong, Seoul 143-701, Korea.
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Summary

The objective of this study was to determine developmental pattern and cell allocation to the inner cell mass and trophectoderm in haploid and diploid embryos following parthenogenetic activation. In vitro matured porcine oocytes were activated by ethanol treatment and cultured in the presence or absence of cytochalasin B for 5h. The oocytes were then cultured in the NCSU23 for 9 days. The combined treatment with cytochalasin B following ethanol treatment did not increase (p >0.1) the incidence of activation. The incidence of development to the blastocyst stage was higher (p <0.05) in the combined treatments of ethanol and cytochalasin B as compared with ethanol treatment alone. The percentage of oocytes with two female pronuclei was higher (p < 0.01) in oocytes treated with cytochalasin B than that in ethanol treatment alone. Treatment with both ethanol and cytochalasin B increased (p <0.01) the incidence of diploid chromosome spread over just the ethanol treatment alone. The average numbers of total cells and inner cell mass were significantly reduced (p <0.05) in the ethanol treatment alone as compared with the combined cytochalasin B and ethanol treatment. These results suggested that the ploidy may affect blastocoele formation and cell allocation to inner cell mass and trophectoderm in the pig.

Keywords

Inner cell massOocytesParthenogenesisPigPloidyTrophectoderm
Type
Article
Information
Zygote , Volume 5 , Issue 4 , November 1997 , pp. 365 - 370
Copyright
Copyright © Cambridge University Press 1997

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