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Activation of amphibian oocytes by sperm extracts

Published online by Cambridge University Press:  01 November 2008

F. Bonilla ,
M. T. Ajmat ,
G. Sánchez Toranzo ,
L. Zelarayán ,
J. Oterino  and
M. I. Bühler
F. Bonilla
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
M. T. Ajmat
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
G. Sánchez Toranzo
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
L. Zelarayán
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
J. Oterino
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
M. I. Bühler*
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina.
*
All correspondence to: Marta I. Bühler. Departamento de Biología del Desarrollo, Chacabuco 461, 4000 – San Miguel de Tucumán, Argentina, Fax: +54 381 4248025. e-mail: [email protected]
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Summary

In the fertilization of most animals, egg activation is accompanied by an increase in cytoplasmatic Ca2+; however, the mechanism through which the fertilizing sperm induce this phenomenon is still controversial. An increase in intracellular free Ca2+ is required to trigger egg activation events, a process that includes cortical granule exocytosis, resumption and completion of meiosis and DNA replication, and culminates in the first mitotic cleavage. In this work, we investigated the effect of microinjection and incubation of different fractions of homologous sperm extract on the activation of Bufo arenarum oocytes matured in vitro. Two heat treatment-sensitive fractions obtained by chromatography were able to induce oocyte activation. The sperm fraction, which contained a 24 kDa protein, induced 90% activation when it was microinjected into the oocytes. Whilst the sperm fraction, which contained a 36 kDa protein, was able to induce about 70% activation only when it was applied on the oocyte surface.

Keywords

Bufo arenarumOocyte activationSperm extracts
Type
Research Article
Information
Zygote , Volume 16 , Issue 4 , November 2008 , pp. 303 - 308
Copyright
Copyright © Cambridge University Press 2008

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