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Molecular approaches for the identification of novel egg components

Published online by Cambridge University Press:  14 March 2007

J. GAUTRON ,
F. NAU ,
K. MANN ,
C. GUERIN-DUBIARD ,
S. REHAULT ,
M.T. HINCKE  and
Y. NYS
J. GAUTRON*
Affiliation:
Unité de Recherches Avicoles, Fonction et Régulation des Protéines de l'oeuf, INRA, UR83, Unité de Recherches Avicoles, F-37380 Nouzilly, France, 37380 Nouzilly, France
F. NAU
Affiliation:
UMR INRA -AgroCampus Rennes 1253 Science et Technologie du Lait et de l'Oeuf, 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France
K. MANN
Affiliation:
Max-Planck-Institut für Biochemie, Abteilung für Proteomics und Signaltransduktion, Martinsried, Germany
C. GUERIN-DUBIARD
Affiliation:
UMR INRA -AgroCampus Rennes 1253 Science et Technologie du Lait et de l'Oeuf, 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France
S. REHAULT
Affiliation:
Unité de Recherches Avicoles, Fonction et Régulation des Protéines de l'oeuf, INRA, UR83, Unité de Recherches Avicoles, F-37380 Nouzilly, France, 37380 Nouzilly, France
M.T. HINCKE
Affiliation:
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, K1H 8M5, Canada
Y. NYS
Affiliation:
Unité de Recherches Avicoles, Fonction et Régulation des Protéines de l'oeuf, INRA, UR83, Unité de Recherches Avicoles, F-37380 Nouzilly, France, 37380 Nouzilly, France
*
*Corresponding author: [email protected]
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Abstract

The avian egg is a nutritious food and also a major source of biologically active compounds that are beneficial for human health. These biologically active molecules are widely used by pharmaceutical, cosmetic and food industries. Egg proteins were previously studied using classical biochemical techniques such as chromatographic and electrophoretic separation and Edman sequence analysis. The development of molecular biology in the late 80's and the recent publication of the chicken genome sequence are major scientific advances leading to identification and characterization of a number of minor egg components that were not previously identified. Using recent data on the characterization of egg white and eggshell matrix proteins, we illustrate in this review recent developments in the biochemistry of the egg (proteomics) and in the molecular biology of the egg (cDNA and ESTs libraries, bioinformatic analysis of the chicken genome, transcriptomics). These methods have allowed the identification of hundreds of minor egg protein components with potential applications for industry. Functional studies to identify the biological activities of these novel egg protein components and to exploit their potential will form the next frontier of egg science.

Keywords

laying henseggsproteinsgenesidentificationESTstranscriptomeproteome
Type
Review Article
Information
World's Poultry Science Journal , Volume 63 , Issue 1 , March 2007 , pp. 82 - 90
Copyright
Copyright © World's Poultry Science Association 2007

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References

ARO, H., ANTON, M., HIIDENHOVI, J., KÖNKÖ, K., JÄRVENPÄÄ, E., HIETANIEMI, V., HUOPALAHTI, R. and RYHÄNEN, E.L. (2004) Egg yolk fractions: Separation by supercritical technologies. In: Proceedings of Cost 923 Workshop; Barcelona (ESP); 2004/10/18–19, 40.Google Scholar
AWADE, A.C., MOREAU, S., MOLLÉ, D., BRULÉ, G. and MAUBOIS, J.L. (1994) Two-step chromatographic procedure for the purification of hen egg white ovomucin, lysozyme, ovotransferrin and ovalbumin and characterization of purified proteins. Journal of Chromatography A 677: 279–288.CrossRefGoogle ScholarPubMed
CASTELLANI, O., MARTINET, V., DAVID-BRIAND, E., GUERIN-DUBIARD, C. and ANTON, M. (2003) Egg yolk phosvitin: Rapid isolation and chromatographic separation using aqueous solvents. Journal of Chromatography B 791: 273–284.CrossRefGoogle Scholar
CROGUENNEC, T., NAU, F., PEZENNEC, S. and BRULE, G. (2000) Simple rapid procedure for preparation of large quantities of ovalbumin. Journal of Agricultural and Food Chemistry 48: 4883–4889.CrossRefGoogle ScholarPubMed
CROGUENNEC, T., NAU, F., PEZENNEC, S., PIOT, M. and BRULE, G. (2001) Two-step chromatographic procedure for the preparation of hen egg white ovotransferrin. European Food Research and Technology 212: 296–301.CrossRefGoogle Scholar
CHAVES, L.D., KNUSTON, T.P., KRUETH, S.B. and REED, K.M. (2005) Using the chicken genome sequence in the development and mapping of genetic markers in the turkey (Meleagris gallopavo). Animal Genetics 37: 130–138.CrossRefGoogle Scholar
DAS, S., MANDAL, M., CHAKRABORTI, T., MANDAL, A. and CHAKRABORTI, S. (2004) Isolation of MMP-2 from MMP-2/TIMP-2 complex: characterization of the complex and the free enzyme in pulmonary vascular smooth muscle plasma membrane. Biochimica et Biophysica Acta 1674: 158–174.Google ScholarPubMed
DÉSERT, C., GUÉRIN-Dubiard, C., NAU, F., JAN, G., VAL, F. and MALLARD, J. (2001) Comparison of different electrophoretic separations of hen egg white proteins. Journal of Agricultural and Food Chemistry 49: 4553–4561.CrossRefGoogle ScholarPubMed
DUNN, I.C. (2005) Breeding strategies to improve the egg's natural defence. World's Poultry Science Journal 60: 458–468.CrossRefGoogle Scholar
GAUTRON, J., BAIN, M., SOLOMON, S. and NYS, Y. (1996) Soluble matrix of hen's eggshell extracts changes in vitro the rate of calcium carbonate precipitation and crystal morphology. British Poultry Science 37: 853–866.CrossRefGoogle Scholar
GAUTRON, J., HINCKE, M.T. and NYS, Y. (1997) Precursor matrix proteins in the uterine fluid change with stages of eggshell formation in hens. Connective Tissue Research 36: 195–210.CrossRefGoogle ScholarPubMed
GAUTRON, J., HINCKE, M.T., PANHELEUX, M., GARCIA-RUIZ, J.M., BOLDICKE, T. and NYS, Y. (2001a) Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer. Connective Tissue Research 42: 255–267.CrossRefGoogle ScholarPubMed
GAUTRON, J., HINCKE, M.T., MANN, K., PANHELEUX, M., BAIN, M., McKEE, M.D., SOLOMON, S.E. and NYS, Y. (2001b) Ovocalyxin-32, a novel chicken eggshell matrix protein: Isolation, amino acid sequencing, cloning and immunocytochemical localization. The Journal of Biological Chemistry 276: 39243–39252.CrossRefGoogle ScholarPubMed
GAUTRON, J., MURAYAMA, E., VIGNAL, A., MORISSON, M., MCKEE, M.D., RÉHAULT, S., VIDAL, M.L., NYS, Y. and HINCKE, M.T. (2007) Cloning of ovocalyxin-36, a novel chicken eggshell protein related to lypopolysaccharide-binding proteins (LBP) bactericidal permeability-increasing proteins (BPI), and plunc family proteins. The Journal of Biological Chemistry, Accepted for publication.CrossRefGoogle Scholar
GUÉRIN-DUBIARD, C., PASCO, M., HIETANEN, A., QUIROS DEL BOSQUE, A., NAU, F. and CROGUENNEC, T. (2005) Hen egg white fractionation by ion-exchange chromatography. Journal of Chromatography A 1090: 58–67.CrossRefGoogle ScholarPubMed
GUÉRIN-DUBIARD, C., PASCO, M., MOLLÉ, D., DÉSERT, C., CROGUENNEC, T. and NAU, F. (2006) Proteomic analysis of hen egg white. Journal of Agricultural and Food Chemistry 54: 3901–3910.CrossRefGoogle ScholarPubMed
HIIDENHOVI, J., ARO, H.S. and KANKARE, V. (1999) Separation of ovomucin subunits by gel filtration: Enhanced resolution of subunits by using a dual-column system. Journal of agricultural and food chemistry 47: 1004–1008.CrossRefGoogle ScholarPubMed
HINCKE, M.T., BERNARD, A.M., LEE, E.R., TSANG, C.P. and NARBAITZ, R. (1992) Soluble protein constituents of the domestic fowl's eggshell. British Poultry Science 33: 505–516.CrossRefGoogle Scholar
HINCKE, M.T. (1995) Ovalbumin is a component of the chicken eggshell matrix. Connective Tissue Research 31: 227–233.CrossRefGoogle ScholarPubMed
HINCKE, M.T., TSANG, C.P., COURTNEY, M., HILL, V. and NARBAITZ, R. (1995) Purification and immunochemistry of a soluble matrix protein of the chicken eggshell (ovocleidin 17). Calcified Tissue International 56: 578–583.CrossRefGoogle ScholarPubMed
HINCKE, M.T., GAUTRON, J., TSANG, C.P., MCKEE, M.D. and NYS, Y. (1999) Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116. The Journal of Biological Chemistry 274: 32915–32923.CrossRefGoogle ScholarPubMed
HINCKE, M.T., GAUTRON, J., PANHELEUX, M., GARCIA-RUIZ, J.M., McKEE, M.D. and NYS, Y. (2000) Identification and localization of lysozyme as a component of the eggshell membranes and shell matrix. Matrix Biology 19: 443–453.CrossRefGoogle Scholar
HINCKE, M.T., GAUTRON, J., MANN, K., PANHELEUX, M., McKEE, M.D., BAIN, M., SOLOMON, S.E. and NYS, Y. (2003) Purification of ovocalyxin-32, a novel chicken eggshell matrix protein. Connective Tissue Research 44 (Sup 1): 16–19.CrossRefGoogle ScholarPubMed
HU, J., ZHANG, X., NOTHNICK, W.B. and SPENCER, T.E. (2004) Matrix metalloproteinases and their tissue inhibitors in the developing neonatal mouse uterus. Biology of Reproduction 71: 1598–1604.CrossRefGoogle ScholarPubMed
INTERNATIONAL CHICKEN GENOME SEQUENCING CONSORTIUM (2004) Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution. Nature 432: 695–716.CrossRefGoogle Scholar
INTERNATIONAL CHICKEN POLYMORPHISM MAP CONSORTIUM (2004) A genetic variation map for chicken with 2.8 million single-nucleotide polymorphisms. Nature 432: 717–722.CrossRefGoogle Scholar
ISHIHAMA, Y., ODA, Y., TABATA, T., SATO, T., NAGASU, T., RAPPSILBER, J. and MANN, M. (2005) Exponentially modified Protein Abundance Index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein. Molecular and Cellular Proteomics 4: 1265–1272.CrossRefGoogle ScholarPubMed
JENSEN, P. (2005) The chicken genome sequence. Heredity 94: 567–568.CrossRefGoogle ScholarPubMed
LI-CHAN, E.C.Y., POWRIE, W.D. and NAKAI, S. (1995) The chemistry of eggs and egg products. In: Egg Science and Technology (Stadelman, W.J., Cotterill, O.J. Eds), pp 105–175. New-York, The Haworth Press.Google Scholar
MANN, M. and STEEN, H. (2004) The ABC's and XYZ's of peptide sequencing. Nature Molecular Cell Biology Reviews 5: 699–711.Google Scholar
MANN, K., MAĚEK, B. and OLSEN, J.V. (2006) Proteomic analysis of the acid-soluble organic matrix of the chicken calcified eggshell layer. Proteomics 6: 3801–3810.CrossRefGoogle Scholar
MINE, Y. and KOVACS-NOLAN, J. (2006) New insights in biologically active proteins and peptides derived from hen egg. World's Poultry Science Journal. 62: 87–95.CrossRefGoogle Scholar
NAU, F., MALLARD, A., PAGES, J. and BRULÉ, G. (1999) Reversed-phase liquid chromatography of egg white proteins. Optimization of ovalbumin elution. Journal of Liquid Chromatography & Related Technologies 22: 1129–1147.CrossRefGoogle Scholar
NAU, F., ANTON, M. and NYS, Y. (2002) The hen's egg: a storehouse for many biologically active molecules. In: Proceedings of the 11th European Poultry Conference; Bremen (DEU); 2002/09/06–10. CD-ROM [/RES/CGR/122/RES/61.pdf]:14 p. Wpsa, German Branch.Google Scholar
NAU, F., GUÉRIN-DUBIARD, C., DÉSERT, C., GAUTRON, J., CARRÉ, W. and LAGARRIGUE, S. (2003a) Identification of three new minor hen egg white proteins. In: Xth European Symposium on the Quality of Eggs and Egg Products. Nys, Y. (ed.); Saint-Brieuc (FRA); 2003/09/23–26. Vol.3: 36–42. Wpsa, Branch Française, Nouzilly (FRA).Google Scholar
NAU, F., GUÉRIN-DUBIARD, C., DÉSERT, C., GAUTRON, J., BOUTON, S., GRIBONVAL, J. and LAGARRIGUE, S. (2003b) Cloning and characterization of HEP21, a new member of the uPAR/Ly6 protein superfamily predominantly expressed in hen egg white. Poultry Science 82: 242–250.CrossRefGoogle ScholarPubMed
NAU, F., PASCO, M., DÉSERT, C., MOLLÉ, D., CROGUENNEC, T. and GUÉRIN-DUBIARD, C. (2005) Identification and characterization of ovalbumin gene Y in hen egg white. Journal of Agricultural and Food Chemistry 53: 2158–2163.CrossRefGoogle ScholarPubMed
NYS, Y. (2001) Composition and nutritional value of the hen's egg. In: Proceedings of the 9th European Congress on the Quality of Egg and Egg Products (Kusadasied) (TUR); 2001/09/09–12. pp 325–341. Wpsa, Turkish Branch.Google Scholar
PAGANO, A.R., CROOIJMANS, M., GROENEN, N., RANDAZZO, N., ZEREGA, B., CANCEDDA, R. and DOZIN, B. (2003) A chondrogenesis-related lipocalin cluster includes a third new gene, CALgamma. Gene 305: 185–194.CrossRefGoogle Scholar
RÉHAULT, S., GAUTRON, J., LABAS, V., BELGHAZI, M. and NYS, Y. (2007) Chicken pro-metalloprotease 2 (pro-MMP-2) is secreted in unfertilized hen egg: new insights in the role of egg white proteins in chicken development. Submitted.Google Scholar
VIGNAL, A. and BESBES, B. (2005) Séquençage du génome de la poule et perspectives d'application en poule pondeuse. In: Proceeding of the 6èmes Journées de la Recherche Avicole; Saint Malo (FRA); 2005/03/30–31. CD-ROM. Itavi, France.Google Scholar
XIAO, Y., HUGHES, A.L., ANDO, J., MATSUDA, Y., CHENG, J.F., SKINNER-NOBLE, D. and ZHANG, G. (2004) Agenome-wide screen identifies a single beta-defensin gene cluster in the chicken: implications for the origin and evolution of mammalian defensins. BMC Genomics 5: 561–11.CrossRefGoogle Scholar
XIAO, Y., CAI, Y., BOMMINENI, Y.R., FERNANDO, S.C., PRAKASH, O., GILLILAND, S.E. and ZHANG, G. (2006) Identification and functional characterization of three chicken cathelicidins with potent antimicrobial activity. The Journal of Biological Chemistry 281: 2858–2867.CrossRefGoogle ScholarPubMed