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Egg-white lysozyme as a food preservative: an overview

Published online by Cambridge University Press:  23 March 2009

F.E. Cunningham
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, Kansas 66506-1600, USA
V.A. Proctor
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, Kansas 66506-1600, USA
S.J. Goetsch
Affiliation:
Department of Animal Sciences and Industry, Kansas State University, Manhattan, Kansas 66506-1600, USA
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Abstract

The use of lysozyme as a food preservative and the factors affecting lysozyme activity (temperature, chemicals, processing and complexes) is reviewed. Lysozyme inhibits the growth of deleterious organisms thus prolonging shelf life. Chemicals used to improve the preservative effect of lysozyme and those that inhibit the enzyme are discussed along with the stability of lysozyme in various chemical environments. Lysozyme has been used to preserve fresh fruits and vegetables, tofu bean curd, seafoods, meats and sausages, potato salad, cooked burdock with soy sauce, and varieties of semi-hard cheeses such as Edam, Gouda and some Italian cheeses. Lysozyme added to infant feeding formulae makes them more closely resemble human milk.

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Reviews
Copyright
Copyright © Cambridge University Press 1991

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References

Aarnes, G. (1974) Substances with antimicrobial effect of importance in cheese-making. Meieriposten 63: 415522Google Scholar
Akashi, A. (1969) Preservative effect of egg-white lysozyme added to cooked sausage. Japanese Journal of Zootechnical Science 40: 243248Google Scholar
Akashi, A. (1970) Preservative effect of egg-white lysozyme on salami sausage. Japanese Journal of Zootechnical Science 41: 143150Google Scholar
Akashi, A. (1971) Preservative effect of egg-white lysozyme on Vienna sausage. Japanese Journal of Zootechnical Science 42: 289295Google Scholar
Akashi, A. (1972a) The effect of egg-white lysozyme on the growth of cheese starter organisms. Journal of the Faculty of Agriculture 17: 6773Google Scholar
Akashi, A. (1972b) Use of egg-white lysozyme for food preservation and its problems. Shokuhin Kogyo 15: 7883Google Scholar
Akashi, A. and Oono, A. (1972) The preservative effect of egg-white lysozyme to non-packaged Kamaboko. Journal of the Agricultural Chemical Society of Japan 46: 177183Google Scholar
Alderton, G., Chen, J.K. and Ito, K.A. (1974) Effect of lysozyme on the recovery of heated Clostridium botulinum spores. Applied Microbiology 27: 613615CrossRefGoogle ScholarPubMed
Ashton, D.H., Wilson, A.A. and Evancho, G.M. (1975) Identification of a component of crystalline egg albumen bactericidal for thermophilic aerobic sporeformers. Applied Microbiology 30: 821824CrossRefGoogle ScholarPubMed
Awad, A., Powria, W.D. and Fennema, O. (1968) Chemical deterioration of frozen bovine muscle at 4°C. Journal of Food Science 33: 227235CrossRefGoogle Scholar
Back, J.F., Oakenfull, D. and Smith, M.B. (1979) Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry 18: 51915196CrossRefGoogle ScholarPubMed
Baker, L.J., Hansen, A.M.F., Rao, P.B. and Bryan, W.P. (1983) Effects of the presence of water on lysozyme conformation. Biopolymers 22: 16371640CrossRefGoogle ScholarPubMed
Bakri, M. and Wolfe, F.H. (1971) Destabilization of casein micelles by lysozyme. Canadian Journal of Biochemistry 49: 882884CrossRefGoogle ScholarPubMed
Beddell, C.R., Blake, C.C.F. and Oatley, S.J. (1975) An X-ray study of the structure and binding properties of iodine-inactivated lysozyme. Journal of Molecular Biology 97: 643654CrossRefGoogle ScholarPubMed
Beychok, S. and Warner, R.C. (1959) Denaturation and electrophoretic behavior of lysozyme. Journal of the American Chemical Society 81: 18921896Google Scholar
Blake, R. (1974) The effect of sodium dodecyl sulfate on the structure of lysozyme. MS Thesis, Kansas State University, Manhattan, KSGoogle Scholar
Bordet, M. (1928) Essais d'extraction du lysozyme. Comptes Rendus de Seances de la Société de Biologie 99: 12541256Google Scholar
Bottazzi, V., Battistotti, B., Bosi, F., Corradini, C. and Dell Acqua, E. (1978) Effect of lysozyme on thermophilic lactic acid bacteria. 20th International Dairy Congress, E, Congrilait, Paris, Brief Communications 527528Google Scholar
Carlsson, B., Cruz, J.R., Garcia, B., Hanson, L.A. and Urrutia, J.J. (1979) Nutrition and Metabolism of the Fetus and Infant (Ed. Visser, H.K.A.), Martinus Nijhoff, The Hague, pp. 263271CrossRefGoogle Scholar
Carroll, E.J. (1979a) The role of lysozyme in killing and lysis of coliform bacteria in the bovine animal. 1. Serum and milk concentrations of lysozyme and susceptibility of coliform strains to its action. Veterinary Microbiology 4: 6172CrossRefGoogle Scholar
Carroll, E.J. (1979b) The role of lysozyme in killing and lysis of coliform bacteria in the bovine animal. 2. Effect of absorbing serum with bentonite or bacteria. Veterinary Microbiology 4: 7384CrossRefGoogle Scholar
Chang, K.Y. and Carr, C.W. (1971) Studies on the structure and function of lysozyme. I. The effect of pH and cation concentration on lysozyme activity. Biochimica et Biophysica Acta 229: 496503CrossRefGoogle ScholarPubMed
Chassy, B.M. and Giuffrida, A. (1980) Method for the lysis of Gram positive asporogenous bacteria with lysozyme. Applied and Environmental Microbiology 39: 153158CrossRefGoogle ScholarPubMed
Chipman, D.M. and Sharon, N. (1969) Mechanism of lysozyme action. Science 165: 454465CrossRefGoogle ScholarPubMed
Cotterill, O.J. and Winter, A.R. (1954) Egg-white lysozyme. 3. The effect of pH on the lysozyme–ovomucin interaction. Poultry Science 34: 679686CrossRefGoogle Scholar
Cunningham, F.E. (1963) Factors affecting the insolubilization of egg-white proteins. PhD Dissertation, University of MissouriGoogle Scholar
Cunningham, F.E. and Cotterill, O.J. (1971) The influence of yolk on egg-white lysozyme. Poultry Science 50: 10131016CrossRefGoogle ScholarPubMed
Cunningham, F.E. and Lineweaver, H. (1965) Stability of egg-white proteins to pasteurizing temperatures above 60°C. Food Technology 19: 136141Google Scholar
Cunningham, F.E. and Lineweaver, H. (1967) Inactivation of lysozyme by native ovalbumin. Poultry Science 66: 14721477Google Scholar
Davies, R.C., Neuberger, A. and Wilson, B.M. (1969) The dependence of lysozyme activity on pH and ionic strength. Biochimica et Biophysica Acta 178: 294305CrossRefGoogle ScholarPubMed
Dubois-Prevost, R. (1970) Dietary uses of lysozyme. Alimentation et la Vie 58: 4449Google Scholar
Ehrenpreis, S. and Warner, R.C. (1956) The interaction of conalbumin and lysozyme. Archives of Biochemistry and Biophysics 61: 187252CrossRefGoogle ScholarPubMed
Eitenmiller, R.R., Barnhart, H.M. and Shahani, K.M. (1971) Effect of gamma irradiation on bovine and human milk lysozymes. Journal of Food Science 36: 11271130CrossRefGoogle Scholar
Feeney, R.E., Macdonnell, L.R. and Ducay, E.D. (1956) Irreversible inactivation of lysozyme by copper. Archives of Biochemistry and Biophysics 61: 7283CrossRefGoogle ScholarPubMed
Fleming, A. and Allison, V.D. (1924) On the antibacterial power of egg-white. Lancet 206: 13031306CrossRefGoogle Scholar
Forsythe, R.R. and Foster, J.F. (1950) Eff-white proteins. I. Electrophoretic studies on whole white. Journal of Biological Chemistry 184: 377383CrossRefGoogle ScholarPubMed
Francis, D. (1980) In: Infant Nutrition (Eds Birch, G.G. and Parker, K.J.), Applied Science Publishers, London, pp. 465485Google Scholar
Frasco, C.B. (1977) Water structure and heat denaturation in lysozyme. PhD Dissertation, Rutgers University, New Brunswick, NJGoogle Scholar
Frasco, C.B., Karmas, E. and Gilbert, S.G. (1978) Water binding patterns of native and denatured lysozyme as determined by infrared spectroscopy. International Congress of Food Science Technical Abstracts, 170Google Scholar
Fujimaki, M., Kato, H. and Hayase, F. (1972) Studies on roasting changes of proteins. I. Changes of casein and lysozyme during roasting. Agricultural and Biological Chemistry 36: 416425Google Scholar
Funes, J., Yong, S. and Karel, M. (1980) Changes in lysozyme due to reactions with volatile products of peroxidizing methyl linoleate. Journal of Agricultural and Food Chemistry 28: 794798CrossRefGoogle ScholarPubMed
Gallo, A.A., Swift, T.J. and Sable, J.Z. (1971) Magnetic resonance study of the Mn2+–lysozyme complex. Biochemical and Biophysical Research Communications 43: 12321238CrossRefGoogle ScholarPubMed
Galyean, R.D., Cotterill, O.J. and Cunningham, F.E. (1972) Yolk inhibition of lysozyme activity in egg-white. Poultry Science 51: 13461353CrossRefGoogle Scholar
Gardner, F.A. and Nikoopour, H. (1969) Growth characteristics of Pseudomonas fluorescens on conalbumin and lysozyme substrates. Poultry Science 48: 4348CrossRefGoogle ScholarPubMed
Genentech, and Genencor, (1985) Cover focus: T4 lysozyme. Food Technology 39: 2Google Scholar
Goldblum, R.M., Garza, C., Johnson, C.A., Harrist, R., Nichols, B.L. and Goldman, A.S. (1981) Human milk banking. I. Effects of container upon immunologic factors in mature milk. Nutrition Research 1: 449459CrossRefGoogle Scholar
Gorini, L. and Felix, F. (1953) Influence du manganese sur la stabilité du lysozyme. I. Influence du manganese sur le vitesse d'inactivation irreversible du lysozyme par la chaleur. Biochimica et Biophysica Acta 10: 128135CrossRefGoogle Scholar
Goudswaard, J., Bakker-De-Koff, E.C. and Ravenswaaij-Kraan, H.P.M. (1978) Lysozyme and its presence in bovine milk and serum. Tijdschrift Voor Diergeneeskunde 103: 445450Google ScholarPubMed
Hall, H.E., Brown, D.F. and Read, R.B. Jr. (1971) Effect of pasteurization on the direct microscopic count of eggs. Journal of Milk and Food Technology 34: 209211CrossRefGoogle Scholar
Hara, S., Murakami, H., Katagiri, K. and Oba, T. (1970) Influence of subculture age and inoculin of hiochi-bacteria on antimicrobial activities of various preservatives against hiochi-bacteria. Journal of the Society of Brewing, Japan (Nippon Jozo Kyokai Zasshi) 65: 10071011Google Scholar
Hartsell, S.E. (1948) Lysozyme activity of rehydrated spray-dried, whole egg-powder. Food Research 13: 136142CrossRefGoogle ScholarPubMed
Hawthorne, J.R. (1950) The action of egg-white lysozyme on ovomucoid and ovomucin. Biochimical et Biophysica Acta 6: 2835CrossRefGoogle ScholarPubMed
Hayase, F., Kato, H. and Fujimaki, M. (1975) Chemical investigations on the changes in lysozyme during heating. Agricultural and Biological Chemistry 39: 12551262Google Scholar
Imoto, T., Sumi, S., Tsuru, M. and Yagishita, K. (1979) An analysis of the interaction of sodium dodecyl sulfate with lysozyme. Agricultural and Biological Chemistry 43: 18091815Google Scholar
Ionesco, H. (1978) Initiation of germination of Clostridium difficein foams. Journal of Food Science 46: 12371240Google Scholar
Itakura, E. (1978) Lysis effect by lysozyme and amoxicillin. Japanese Journal of Dermatology 88: 1722Google Scholar
Kanner, J. and Karel, M. (1976) Changes in lysozyme due to reactions with peroxidizing methyl linoleate in a dehydrated model system. Journal of Agricultural and Food Chemistry 24: 468472CrossRefGoogle Scholar
Kisza, J., Panfil, H. and Ziajka, S. (1974) Influence of added lysozyme on pepsin digestion of milk protein. Proceedings of the 19th International Dairy Congress 1E: 565Google Scholar
Klotz, I.M. and Walker, E.M. (1948) Complexes of lysozyme. Archives of Biochemistry and Biophysics 18: 319325Google ScholarPubMed
Knorr, D., Shelly, K.J., Hood, L.F. and Kinsella, J.E. (1979) An enzymatic method for yeast autolysis. Journal of Food Science 44: 13621365CrossRefGoogle Scholar
Korkohen, H. (1978) Effect of lactoferrin and lysozyme in milk on growth inhibition of Bacillus stearothermophilus in thermocult method. Suomen ElaeinlaeaekaerilehiGoogle Scholar
Koterska, B., Poznanska, S., Lewicki, C. and Ryduzik, W. (1972) Inhibition of butyric acid fermentation in cheese by addition of lysozyme in the form of egg-white. Dodatek Naukowy 4: 57Google Scholar
Kralovic, R.C. (1973) Clostridium botulinum type E spores: increased heat resistance through lysozyme treatment. Abstracts of Annual Meeting of the American Society for Microbiology 73: 41Google Scholar
Kravchenko, N.A., Kagramanova, K.A. and Kuznetzov, Y.D. (1967) Effect of salts and other factors on lysozyme activity. Biokhimiia 32: 618623Google ScholarPubMed
Kume, T., Sato, Y. and Umemoto, Y. (1973) Effect of irradiation on egg-white lysozyme in aqueous solution. Journal of the Agricultural Chemical Society of Japan (Nihon Nogei Kagakkaishi) 47: 549555Google Scholar
Lin, T.Y. (1970) Lactose synthetase. Modification of carboxyl groups in α-lactalbumin. Biochemistry 9: 984985CrossRefGoogle ScholarPubMed
Linz, R. (1937) Essai de purification du lysozyme de blanc d'oeuf. Comptes Rendus de Seances de la Société de Biologie 126: 12791280Google Scholar
Litwack, G. and Sears, M.L. (1965) Precipitation of lysozyme with the thyroxine or thyroglobulin. Journal of Biological Chemistry 240: 674678CrossRefGoogle ScholarPubMed
McDonald, C.C. and Phillips, W.D. (1969) Perturbation of the PMR spectrum of lysozyme by Co2+. Biochemical and Biophysical Research Communications 35: 4351CrossRefGoogle Scholar
Meyer, K., Thompson, R., Palmer, J.W. and Khorazo, D. (1936) The purification and properties of lysozyme. Journal of Biological Chemistry 113: 303309CrossRefGoogle Scholar
Mineyeva, L.A., Seminova, L.R. and Gusev, M.V. (1979) The effect of lysozyme, EDTA, magnesium and mannitol on spheroplast formation by Anacystic nidulans. Mikrobiologiia 48: 693698Google Scholar
Mohamed, S.Y. (1969) The effect of ionizing radiation on selected chemical, physical and microbiological characteristics of egg-white proteins. PhD Dissertation, Texas A.M. University, College Station, TXGoogle Scholar
Mustafa, A.A. (1963) Lysozyme effect on some members of genus Clostridium. MS Thesis, Kansas State University, Manhattan, KSGoogle Scholar
Nakagawa, S. and Maeshigi, S. (1980) Preservative effects of an egg-white lysozyme–glycine mixture and a hydrolyzed animal protein preparation on potato salad, seasoned burdock, and sauce for fried fish. Hiroshima-ken Shokuhin Kogyo Shekenjo Kenkyu Hokoku 15: 2732Google Scholar
Ng, H. and Garibaldi, J.A. (1975) Death of Staphylococus aureus in liquid whole egg near pH 8.0. Applied Microbiology 29: 782786CrossRefGoogle Scholar
Nichol, L.W. and Winzor, D.T. (1964) The determination of equilibrium constants from rapidly reacting systems of the type A + B. Canadian Journal of Physical Chemistry 68: 24552463CrossRefGoogle Scholar
Nielsen, E.W. (1980) Is saltpetre dispensible in cheesemaking? Maelkeritidende 93: 97102Google Scholar
Nishihava, K. and Isoda, K. (1967) Fecal bacterial flora in the use of lysozyme treated dried milk. Acta Paediatrica Japonica 71: 95102Google Scholar
Panfil-Kuncewicz, H. and Kisza, J. (1976) Effect of lysozyme on microflora development in cowapos;s milk. Zeszyty Naukowe Akademii Rolniczo-Technicznej w Olsztynie (Technologia Zywnosci 10) 164: 3746Google Scholar
Panfil-Kuncewicz, H. and Kisza, J. (1978) Effect of lysozyme on proteolytic susceptibility of cow's milk. Zeszyty Naukowe Akademii Rolniczo-Technicznej w Olsztynie (Technologia Zywnosci 12) 178: 3746Google Scholar
Parkinson, T.L. (1967) Effect of pasteurization on the chemical composition of liquid whole egg. I. Development of a scheme for the fractionation of the proteins of whole egg. Journal of the Science of Food and Agriculture 18: 208213CrossRefGoogle ScholarPubMed
Peterson, R.G. and Hartsell, S.E. (1955) The lysozyme spectrum of the Gram negative bacteria. Journal of Infectious Diseases 96: 7581CrossRefGoogle ScholarPubMed
Poncelet, F. and Catteau, M. (1975) Use of lysozyme for counting and identifying Clostridium perfringens in foods. Microbiological Association des Diplomes de Microbiologie de la Faculté de Pharmacie de Nancy 1: 5359Google Scholar
Reed, G. (1975) Proteolytic enzymes. In: Enzymes in Food Processing, Academic Press, New York, pp. 502504Google Scholar
Ross, V. (1949) Precipitation of insulin by lysozyme and effect of the complex on the blood sugar level. Society for Experimental Biology, Medicine Proceedings 72: 465468CrossRefGoogle ScholarPubMed
Rupley, J.A. (1967) The binding and cleavage by lysozyme of N-acetyl-glucosamine oligosaccharides. Proceedings of the Royal Society, Ser. B 167: 416428Google ScholarPubMed
Safonova, T.B., Afanasieva, T.I., Kravchenko, N.A., Cherkasov, I.A. and Sobolev, V.R. (1978) Egg lysozyme effect on representatives of Micrococcaceae. Effect on micrococci. Antibiotiki 23: 797802Google Scholar
Safonova, T.B., Afanasieva, T.I., Kravchenko, N.A., Cherkasov, I.A. and Sobolev, V.R. (1979) Effect of egg lysozyme on Micrococcaceae. Effect on staphylococci. Antibiotiki 24: 508511Google ScholarPubMed
Safonova, T.B., Sobolev, V.R., Afanasievam, T.I., Kravchenko, N.A. and Cherkasov, I.A. (1981) Egg lysozyme effect on micrococci and staphylococci. Antibiotiki 26: 264268Google ScholarPubMed
St. John, J.L. and Flor, I.H. (1931) A study of whipping and coagulation of eggs of varying quality. Poultry Science 10: 7182CrossRefGoogle Scholar
Salton, M.R.J. (1956) Studies of the bacterial cell wall. V. The action of lysozyme on cell walls of some lysozyme-sensitive bacteria. Biochimica et Biophysica Acta 22: 495506CrossRefGoogle ScholarPubMed
Salton, M.R.J. (1957) The properties of lysozyme and its action on microorganisms. Bacteriological Reviews 21: 82100CrossRefGoogle ScholarPubMed
Salton, M.R.J. and Pavilik, J.G. (1960) Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme. Biochimica et Biophysica Acta 39: 398407CrossRefGoogle ScholarPubMed
Sandow, A. (1926) The antibacterial activity of egg-white. Proceedings of the Society for Experimental Biology and Medicine 24: 172CrossRefGoogle Scholar
Sato, Y., Umemoto, Y. and Kume, T. (1969) The effects of gamma-irradiation on egg-white proteins. Food Irradiation (Shokuhin-Shosha) 4: 4246CrossRefGoogle Scholar
Schaich, K. (1974) Free radical formation in proteins exposed to peroxidizing lipids. ScD Dissertation, Massachusetts Institute of Technology, Cambridge, MAGoogle Scholar
Schaich, K.M. and Karel, M. (1975) Free radicals in lysozyme reacted with peroxidizing methyl linoleate. Journal of Food Science 40: 456459CrossRefGoogle Scholar
Scott, D. (1956) Miscellaneous applications of enzymes. Cited In: Enzymes in Food Processing (Ed. Reed, G.), Academic Press, New York (1975)Google Scholar
Shahani, K.M. (1970) Isolation, characterization and significance of lysozyme and lipase in milk. Proceedings of the 18th International Dairy Congress, Sydney 1E: 61Google Scholar
Silberstein, S. and Inouye, M. (1974) The effect of lysozyme on DNA-membrane association in Escherichia coli. Biochimica et Biophysica Acta 366: 149158CrossRefGoogle ScholarPubMed
Smolelis, A.N. and Hartsell, S.E. (1952) Factors affecting the lytic activity of lysozyme. Journal of Bacteriology 63: 665674CrossRefGoogle ScholarPubMed
Steiner, R.F. (1953) Reversible association processes of globular proteins. II. Electrostatic complexes of plasma albumin and lysozyme. Archives of Biochemistry and Biophysics 47: 5675CrossRefGoogle ScholarPubMed
Swan, I.D.A. (1972) The inhibition of hen egg-white lysozyme by imidazole and indole derivatives. Journal of Molecular Biology 65: 5962CrossRefGoogle ScholarPubMed
Teichberg, V.I., Sharon, N., Moult, J., Smilansky, A. and Yonath, A. (1974) Binding of divalent copper ions to aspartic acid residue 52 in hen egg-white lysozyme. Journal of Molecular Biology 87: 357368CrossRefGoogle ScholarPubMed
Teotia, J.S. and Miller, B.F. (1975) Destruction of Salmonella on poultry meat with lysozyme, EDTA, X-ray, microwave and chlorine. Poultry Science 54: 13881394CrossRefGoogle ScholarPubMed
Ting, D.C.Y. and Schrier, E.E. (1977) Binding of mercury (II) ion to hen egg-white lysozyme and bovine pancreatic ribonuclease A. Journal of Agricultural and Food Chemistry 25: 158162CrossRefGoogle Scholar
Trentini, W.C. and Murray, R.G. (1975) Ultrastructural effects of lysozymes on the cell wall of Caryophanon latum. Canadian Journal of Microbiology 21: 164172CrossRefGoogle ScholarPubMed
Tribout, M. and Leonis, J. (1975) Hydrogen ion titration of lysozyme in alcohol-water solutions. Biochimica et Biophysica Acta 405: 8288CrossRefGoogle ScholarPubMed
Uchida, M., Yokomura, S. and Nagahama, G. (1972) Studies on lactobacilli isolated from mirin liquor. V. Antiseptic effects of egg-white lysozyme on the growth of lactobacilli in mirin liquor. Journal of Fermentation Technology 50: 292297Google Scholar
Vakil, J.R., Chandan, R.C., Parry, R.M. and Shahani, K.M. (1969) Susceptibility of several microorganisms to milk lysozymes. Journal of Dairy Science 52: 11921197CrossRefGoogle Scholar
Vedmina, E.A., Pasternak, N.A., Shenderovich, V.A. et al. (1979) Sensitivity of Gram-negative microflora to lysozyme. Antibiotiki 124: 746750Google Scholar
Wasserfall, F. (1976) Can nitrate be omitted in cheese manufacture? Deutsche Molkerei-Zeitung 97: 677680Google Scholar
Wasserfall, F. (1977) Principles of the effect of lysozyme in the manufacture of semi-hard cheese. I. Accuracy of determination recovery and stability of lysozyme. Milchwissenschaft 32: 654657Google Scholar
Wasserfall, F. (1978) Principles of the effect of lysozyme in the manufacture of semi-hard cheese. II. Influence of lysozyme on starter cultures and coliforms. Milchwissenschaft 33: 9196Google Scholar
Wasserfall, F. and Teuber, M. (1979) Action of egg-white lysozyme on Clostridium tyrobutyricum. Applied and Environmental Microbiology 38: 197199CrossRefGoogle ScholarPubMed
Wasserfall, F.E., Voss, E. and Prokopek, D. (1976) Studies on cheese ripening. V. The use of lysozyme instead of nitrate to inhibit late blowing of cheese. Kieler Milchwirtschaftliche Forschungsberichte 28: 316Google Scholar
Weaver, G.L. and Kroger, M. (1978) Lysozyme activity of high-leucocyte-count milk and the effect of heat and potassium dichromate on lysozyme activity. Journal of Dairy Science 61: 10891092CrossRefGoogle ScholarPubMed
Wooley, R.E. and Blue, J.L. (1975) In vitro effect of EDTA-Tris-lysozyme solutions on selected pathogenic bacteria. Journal of Medical Microbiology 8: 189194CrossRefGoogle ScholarPubMed
Wu, A.C.M. (1975) Study of activities and stabilities of yeast invertase, bovine-pancreas chymotrypsin, lysozyme, and the stability of soy protein extract under fluctuation temperature. PhD Dissertation. University of Georgia, Athens, GA. (Diss. Abst. Intern. B. 35: 5936)Google Scholar
Wu, A.C.M., Eitenmiller, R.R. and Powers, J.J. (1975) Yields from chymotrypsin and lysozyme under fluctuating temperature treatments. Journal of Food Science 40: 840843CrossRefGoogle Scholar
Yajima, M., Hidaka, Y. and Matsuoka, Y. (1968) Studies on egg-white lysozyme as a preservative of sake. Journal of Fermentation Technology 46: 782788Google Scholar
Yajima, M., Hidaka, Y. and Matsuoka, Y. (1971) Studies on egg-white lysozyme as a preservative of sake. II. Journal of Fermentation Technology 49: 693698Google Scholar
Yashitake, S. and Shinichiro, A. (1977) Use of egg-white lysozyme in the food industry. New Food Industry 19: 1723Google Scholar