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Current loop-mediated isothermal amplification (LAMP) technologies for the detection of poultry pathogens

Published online by Cambridge University Press:  26 February 2018

S. EHTISHAM-UL-HAQUE ,
M.A. ZAMAN ,
M. KIRAN ,
M.K. RAFIQUE ,
M.F. QAMAR  and
M. YOUNUS
S. EHTISHAM-UL-HAQUE*
Affiliation:
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang campus), Jhang-35200, Pakistan
M.A. ZAMAN
Affiliation:
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang campus), Jhang-35200, Pakistan
M. KIRAN
Affiliation:
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang campus), Jhang-35200, Pakistan
M.K. RAFIQUE
Affiliation:
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang campus), Jhang-35200, Pakistan
M.F. QAMAR
Affiliation:
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang campus), Jhang-35200, Pakistan
M. YOUNUS
Affiliation:
Department of Pathobiology, University of Veterinary and Animal Sciences, Lahore, (Jhang campus), Jhang-35200, Pakistan
*
Corresponding author: [email protected]
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Abstract

Traditional diagnostic capabilities (serology and culture) are not enough to monitor the poultry infections efficiently. For effective control of poultry infections, a regular program incorporating simple and cost-effective molecular diagnostics is necessary. On this rationale, it is possible to present working molecular diagnostic technology that would work equally well in field as well as in the laboratory. Recently, the loop-mediated isothermal amplification (LAMP) assay has emerged as simple and inexpensive diagnostic tool for the molecular detection of various animal pathogens. To perform LAMP, no specialised instruments (e.g. thermal cycler) are required, permitting its use in developing countries. Various reliable LAMP assays have been reported for the detection of different poultry pathogens. However, still there is a need to improve the sensitivity, specificity, reproducibility, user-friendliness, delivery to end-user and affordability of LAMP assays. This article reviews current LAMP assays available for the molecular detection of important viral, bacterial and protozoan pathogens of poultry. It focuses on the various aspects of LAMP for the diagnosis of important poultry pathogens based upon pathogen type, specimen, target genes, LAMP primer types, detection limits, fluorescent detectors and LAMP chemistry used. This paper provides updates on principle, instrumentation, basic methodology, quantification capability, reagents and kits used currently in performing LAMP.

Keywords

LAMPdiseasespoultrymolecular diagnostics
Type
Review
Information
World's Poultry Science Journal , Volume 74 , Issue 2 , June 2018 , pp. 287 - 300
Copyright
Copyright © World's Poultry Science Association 2018 

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References

ANGAMUTHU, R., BASKARAN, S., GOPAL, D.R., DEVARAJAN, J. and KATHAPERUMAL, K. (2012) Rapid detection of the Marek's disease viral genome in chicken feathers by loop-mediated isothermal amplification. Journal of Clinical Microbiology 50: 961-965.Google Scholar
BAO, H., WANG, X., ZHAO, Y., SUN, X., LI, Y., XIONG, Y. and CHEN, H. (2012) Development of a reverse transcription loop-mediated isothermal amplification method for the rapid detection of avian influenza virus subtype H7. Journal of Virological Methods 179: 33-37.Google Scholar
BAO, H., ZHAO, Y., WANG, Y., XU, X., SHI, J., ZENG, X., WANG, X. and CHEN, H. (2014) Development of a reverse transcription loop-mediated isothermal amplification method for the rapid detection of subtype H7N9 avian influenza virus. BioMed Research International 2014, Article ID 525064.CrossRefGoogle Scholar
BARKWAY, C.P., POCOCK, R.L., VRBA, V. and BLAKE, D.P. (2015) Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens. Journal of Visualised Experiments: JoVE 96: 52552.Google Scholar
BARKWAY, C.P., POCOCK, R.L., VRBA, V. and BLAKE, D.P. (2011) Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens. BMC Veterinary Research 7: 67.CrossRefGoogle ScholarPubMed
BBSRC (2013) Control of diseases in livestock is integral to food security. Food security cannot be taken for granted. The Pirbright Institute, UK. http://pirbright.ac.uk/foodsecurity/Default.aspx.Google Scholar
BHIMANI, M., BHENDERI, B. and ROY, A. (2015) Loop-mediated isothermal amplification assay (LAMP) based detection of Pasteurella multocida in cases of haemorrhagic septicaemia and fowl cholera. Veterinaria Italiana 51: 115-121.Google Scholar
CHANDRASEKAR, A., RAJA, A., DHINAKAR RAJ, G., THANGAVELU, A. and KUMANAN, K. (2015) Rapid detection of Avian Infectious Bronchitis virus by reverse transcriptase-Loop mediated isothermal amplification. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 85: 815-820.Google Scholar
CHEN, H.-T., ZHANG, J., MA, Y.-P., MA, L.-N., DING, Y.-Z., LIU, X.-T., CAI, X.-P., MA, L.-Q., ZHANG, Y.-G. and LIU, Y.-S. (2010) Reverse transcription loop-mediated isothermal amplification for the rapid detection of infectious bronchitis virus in infected chicken tissues. Molecular and Cellular Probes 24: 104-106.Google Scholar
CHEN, H.-T., ZHANG, J., SUN, D.-H., MA, L.-N., LIU, X.-T., CAI, X.-P. and LIU, Y.-S. (2008) Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus. Journal of Virological Methods 151: 200-203.Google Scholar
EHTISHAM-UL-HAQUE, S., KIRAN, M., WAHEED, U. and YOUNUS, M. (2017) Real-time loop-mediated isothermal amplification (LAMP) of mgc2 gene of Mycoplasma gallisepticum. Journal of Veterinary Research 61: 439-444.Google Scholar
GE, Y., WU, B., QI, X., ZHAO, K., GUO, X., ZHU, Y., QI, Y., SHI, Z., ZHOU, M. and WANG, H. (2013) Rapid and sensitive detection of novel avian-origin influenza a (H7N9) virus by reverse transcription loop-mediated isothermal amplification combined with a lateral-flow device. PLoS One 8: e69941.Google Scholar
GONG, J., ZHUANG, L., ZHU, C., SHI, S., ZHANG, D., ZHANG, L., YU, Y., DOU, X., XU, B. and WANG, C. (2016) Loop-mediated isothermal amplification of the sefA gene for rapid detection of Salmonella enteritidis and Salmonella gallinarum in chickens. Foodborne Pathogens and Disease 13: 177-181.Google Scholar
HELAL, Z.H., LI, J., GIRSHICK, T. and KHAN, M.I. (2017) Duplex RT-Loop-Mediated Isothermal Amplification Assay for simultaneous detection of Influenza A Virus H6 and H9 Subtypes. Annals of Virology and Research 3 (1): 1026.Google Scholar
HU, Y., XU, P., LUO, J., HE, H. and DU, W. (2017) Absolute quantification of H5-subtype Avian Influenza viruses using droplet digital Loop-Mediated Isothermal Amplification. Analytical Chemistry 89: 745-750.Google Scholar
HUANG, C.H., LAI, G.H., LEE, M.S., LIN, W.H., LIEN, Y.Y., HSUEH, S.C., KAO, J.Y., CHANG, W.T., LU, T.C. and LIN, W.N. (2010) Development and evaluation of a loop‐mediated isothermal amplification assay for rapid detection of chicken anaemia virus. Journal of Applied Microbiology 108: 917-924.Google Scholar
KIRUNDA, H., THEKISOE, O., KASAIJA, P., KERFUA, S., NASINYAMA, G., OPUDA-ASIBO, J. and INOUE, N. (2012) Use of reverse transcriptase loop-mediated isothermal amplification assay for field detection of newcastle disease virus using less invasive samples. Veterinary World 5: 206-212.CrossRefGoogle Scholar
KURSA, O., WOŹNIAKOWSKI, G., TOMCZYK, G., SAWICKA, A. and MINTA, Z. (2014) Rapid detection of Mycoplasma synoviae by loop-mediated isothermal amplification. Archives of Microbiology 197: 319-325.Google Scholar
LAMP PRIMER DESIGN SOFTWARE (PRIMER EXPLORER V4) http://primerexplorer.jp/elamp4.0.0/index.html.Google Scholar
LEE, M.-S., LIN, Y.-C., LAI, G.-H., LAI, S.-Y., CHEN, H.-J. and WANG, M.-Y. (2011) One-step reverse-transcription loop-mediated isothermal amplification for detection of infectious bursal disease virus. Canadian Journal of Veterinary Research, 75 (2): 122-127.Google Scholar
LI, Q., XUE, C., QIN, J., ZHOU, Q., CHEN, F., BI, Y. and CAO, Y. (2009) An improved reverse transcription loop-mediated isothermal amplification assay for sensitive and specific detection of newcastle disease virus. Archives of Virology 154: 1433-1440.Google Scholar
LUO, S.-S., XIE, Z.-X., DENG, X.-W., XIE, Z.-Q., PANG, Y.-S., XIE, L.-J., LIU, J.-B., FAN, Q. and PENG, Y. (2012) Development of a loop-mediated isothermal amplification assay for visual detection of Mycoplasma gallisepticum. China Animal Husbandry and Veterinary Medicine 9: 008.Google Scholar
MANSOUR, S.M., ALI, H., CHASE, C.C. and CEPICA, A. (2015) Loop-mediated isothermal amplification for diagnosis of 18 world organisation for animal health (OIE) notifiable viral diseases of ruminants, swine and poultry. Animal Health Research Reviews 16: 89-106.CrossRefGoogle ScholarPubMed
MAIR, G., VILEI, E.M., WADE, A., FREY, J. and UNGER, H. (2013) Isothermal loop-mediated amplification (LAMP) for diagnosis of contagious bovine pleuro-pneumonia. BMC Veterinary Research 9: 1.Google Scholar
MORI, Y., KITAO, M., TOMITA, N. and NOTOMI, T. (2004) Real-time turbidimetry of LAMP reaction for quantifying template DNA. Journal of Biochemical and Biophysical Methods 59: 145-157.Google Scholar
NOTOMI, T., OKAYAMA, H., MASUBUCHI, H., YONEKAWA, T., WATANABE, K., AMINO, N. and HASE, T. (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Research 28: e63-e63.Google Scholar
OIE (2007) Prevention and control of animal diseases worldwide. Part I: Economic analysis: prevention versus outbreak costs. Civic consulting. The world organisation for animal health (OIE).Google Scholar
OKAMURA, M., OHBA, Y., KIKUCHI, S., SUZUKI, A., TACHIZAKI, H., TAKEHARA, K., IKEDO, M., KOJIMA, T. and NAKAMURA, M. (2008) Loop-mediated isothermal amplification for the rapid, sensitive, and specific detection of the O9 group of salmonella in chickens. Veterinary Microbiology 132: 197-204.CrossRefGoogle ScholarPubMed
OKAMURA, M., OHBA, Y., KIKUCHI, S., TAKEHARA, K., IKEDO, M., KOJIMA, T. and NAKAMURA, M. (2009) Rapid, sensitive, and specific detection of the O4 group of Salmonella enterica by loop-mediated isothermal amplification. Avian Diseases 53: 216-221.Google Scholar
PATEL, J.C., LUCCHI, N.W., SRIVASTAVA, P., LIN, J.T., SUG-ARAM, R., ARUNCHARUS, S., BHARTI, P.K., SHUKLA, M.M., CONGPUONG, K., SATIMAI, W., SINGH, N., UDHAYAKUMAR, V. and MESHNICK, S.R. (2014) Field evaluation of a real-time fluorescence loop-mediated isothermal amplification assay, realAmp, for the diagnosis of malaria in Thailand and India. Journal of Infectious Diseases 210: 1180-1187.Google Scholar
PENG, Y., XIE, Z., LIU, J., PANG, Y., DENG, X., XIE, Z., XIE, L., FAN, Q., FENG, J. and KHAN, M.I. (2011) Visual detection of H3 subtype avian influenza viruses by reverse transcription loop-mediated isothermal amplification assay. Virology Journal 8: 1-10.Google Scholar
PHAM, H.M., NAKAJIMA, C., OHASHI, K. and ONUMA, M. (2005) Loop-mediated isothermal amplification for rapid detection of newcastle disease virus. Journal of Clinical Microbiology 43: 1646-1650.Google Scholar
RAMOS, A.E., MUÑOZ, M., CORTÉS-VECINO, J.A., BARATO, P. and PATARROYO, M.A. (2017) A novel loop-mediated isothermal amplification-based test for detecting Neospora caninum DNA. Parasites and Vectors 10: 590.CrossRefGoogle ScholarPubMed
SABIKE, I.I., UEMURA, R., KIRINO, Y., MEKATA, H., SEKIGUCHI, S., OKABAYASHI, T., GOTO, Y. and YAMAZAKI, W. (2016) . Use of direct LAMP screening of broiler faecal samples for Campylobacter jejuni and Campylobacter coli in the positive flock identification strategy. Frontiers in Microbiology 7: 1582.Google Scholar
SAITO, R., MISAWA, Y., MORIYA, K., KOIKE, K., UBUKATA, K. and OKAMURA, N. (2005) Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae. Journal of Medical Microbiology 54: 1037-1041.Google Scholar
SPRYGIN, A., ANDREYCHUK, D., KOLOTILOV, A., VOLKOV, M., RUNINA, I., MUDRAK, N., BORISOV, A., IRZA, V., DRYGIN, V. and PEREVOZCHIKOVA, N. (2010) Development of a duplex real-time Taqman PCR assay with an internal control for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from commercial and backyard poultry. Avian Pathology 39: 99-109.Google Scholar
TAKAHASHI, R., FUKUTA, S., KUROYANAGI, S., MIYAKE, N., NAGAI, H., KAGEYAMA, K. and ISHIGURO, Y. (2014) Development and application of a loop-mediated isothermal amplification assay for rapid detection of Pythium helicoides. FEMS Microbiology Letters 355: 28-35.CrossRefGoogle ScholarPubMed
TSAI, S.-M., LIU, H.-J., SHIEN, J.-H., LEE, L.-H., CHANG, P.-C. and WANG, C.-Y. (2012) Rapid and sensitive detection of infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. Journal of Virological Methods 181: 117-124.CrossRefGoogle ScholarPubMed
WANG, Y., KANG, Z., GAO, H., GAO, Y., QIN, L., LIN, H., YU, F., QI, X. and WANG, X. (2011) A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus. Virology Journal 8: 1.Google Scholar
WEI, X., SHI, X., ZHAO, Y., ZHANG, J., WANG, M., LIU, C., CUI, H., HU, S., QUAN, Y. and CHEN, H. (2012) Development of a rapid and specific loop-mediated isothermal amplification detection method that targets Marek's disease virus meq gene. Journal of Virological Methods 183: 196-200.Google Scholar
WORLD HEALTH ORGANISATION (2013) The use of a commercial Loop-Mediated Isothermal Amplification Assay (TB-LAMP) for the detection of Tuberculosis. WHO Expert Group Meeting Report Geneva. 2013, 1-50.Google Scholar
WOZNIAKOWSKI, G., SAMOREK-SALAMONOWICZ, E. and KOZDRUN, W. (2011) Rapid detection of Marek's disease virus in feather follicles by loop-mediated amplification. Avian Diseases 55: 462-467.Google Scholar
XIE, Q.-M., JI, J., PICKENS, T.T., DU, L.-Q., CAO, Y.-C., LI, H.-M., WANG, L.-G., MA, J.-Y. and BI, Y.-Z. (2010) Rapid detection of infectious laryngotracheitis virus isolates by loop-mediated isothermal amplification. Journal of Virological Methods 165: 71-75.Google Scholar
XIE, Z., PENG, Y., LUO, S., WANG, Y., LIU, J., PANG, Y., DENG, X., XIE, Z., XIE, L. and FAN, Q. (2012) Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus. Avian Pathology 41: 311-316.Google Scholar
XIE, Z., TANG, Y., FAN, Q., LIU, J., PANG, Y., DENG, X., XIE, Z., PENG, Y., XIE, L. and KHAN, M.I. (2011) Rapid detection of group I avian adenoviruses by a loop-mediated isothermal amplification. Avian Diseases 55: 575-579.Google Scholar
XU, J., ZHANG, Z., YIN, Y., CUI, S., XU, S., GUO, Y., LI, J., WANG, J., LIU, X. and HAN, L. (2009) Development of reverse-transcription loop-mediated isothermal amplification for the detection of infectious bursal disease virus. Journal of Virological Methods 162: 267-271.Google Scholar
XU, J., QU, C. and TAO, J. (2014) Loop-mediated isothermal amplification assay for detection of Histomonas meleagridis infection in chickens targeting the 18S rRNA sequences. Avian Pathology 43: 62-67.CrossRefGoogle ScholarPubMed
XUE, C., ZHANG, Y., ZHOU, Q., XU, C., LI, X. and CAO, Y. (2009) Rapid detection of infectious bursal disease virus by reverse transcription loop-mediated isothermal amplification assay. Journal of Veterinary Diagnostic Investigation 21: 841-843.Google Scholar
YAMAZAKI, W., TAGUCHI, M., KAWAI, T., KAWATSU, K., SAKATA, J., INOUE, K. and MISAWA, N. (2009) Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples. Applied and Environmental Microbiology 75: 1597-1603.Google Scholar
YOUN, S.Y., JEONG, O.M., CHOI, B.K., JUNG, S.C. and KANG, M.S. (2017) Application of loop-mediated isothermal amplification with propidium monoazide treatment to detect live Salmonella in chicken carcasses. Poultry Science 96: 458-464.Google Scholar
ZHANG, F., BAO, S., YU, S., CHENG, J., TAN, L., QIU, X., SONG, C., DAI, Y., FEI, R. and DING, C. (2015) Development of a loop-mediated isothermal amplification targeting a gene within the pyruvate dehydrogenase complex, the pdhA gene, for rapid detection of Mycoplasma gallisepticum. Journal of Veterinary Diagnostic Investigation 27: 260-267.Google Scholar
ZHUANG, L., GONG, J., LI, Q., ZHU, C., YU, Y., DOU, X., LIU, X., XU, B. and WANG, C. (2014) Detection of salmonella spp. By a loop-mediated isothermal amplification (lamp) method targeting bcfD gene. Letters in Applied Microbiology 59: 658-664.Google Scholar