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The Biosynthesis of N-(3-Carboxy-2,5-Dichlorophenyl)-Glucosylamine in Plant Tissue Sections

Published online by Cambridge University Press:  12 June 2017

D. S. Frear ,
C. R. Swanson  and
R. E. Kadunce
D. S. Frear
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota
C. R. Swanson
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota
R. E. Kadunce
Affiliation:
Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, Metabolism and Radiation Research Laboratory, State University Station, Fargo, North Dakota
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Abstract

A quantitative colorimetric procedure was developed for the determination of N-glucosyl amiben in tissue sections of etiolated seedlings. N-glucosyl amiben biosynthesis in soybean hypocotyl sections was heat labile, sensitive to freezing and thawing, unaffected under anaerobic conditions, dependent on amiben concentration, and proportional to tissue section concentration. The formation of N-glucosyl amiben in soybean hypocotyl sections was greatest between pH 6.3 and pH 7.3 and was inhibited by NaF, HgCl2, iodoacetate, iodoacetamide, and N-ethylmaleimide but not by NaCN or NaN3. On a specific activity basis, sugar beet seedling, soybean cotyledon, and soybean hypocotyl tissue sections were the most active tissues studied in the biosynthesis of N-glucosyl amiben.

Type
Research Article
Information
Weeds , Volume 15 , Issue 2 , April 1967 , pp. 101 - 104
Copyright
Copyright © 1967 Weed Science Society of America 

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References

Literature Cited

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