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Johnsongrass (Sorghum halepense) Pollen Expresses ACCase Target-Site Resistance

Published online by Cambridge University Press:  20 January 2017

Ian C. Burke ,
James B. Holland ,
James D. Burton ,
Alan C. York  and
John W. Wilcut
Ian C. Burke
Affiliation:
Crop Science Department, North Carolina State University, Box 7620 Raleigh, NC 27695-7620
James B. Holland
Affiliation:
USDA–ARS, Plant Science Research Unit, Crop Science Department, North Carolina State University, Box 7620 Raleigh, NC 27695-7620
James D. Burton
Affiliation:
Horticulture Department, North Carolina State University, Raleigh, NC 27695-7609
Alan C. York
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
John W. Wilcut*
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
*
Corresponding author's E-mail: [email protected]
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Abstract

Three studies were conducted to develop pollen tests for the screening of acetyl coenzyme-A carboxylase (ACCase) target-site resistance in a biotype of johnsongrass. The assays were based on germination of johnsongrass pollen in media supplemented with clethodim. Two different methods were used to evaluate pollen germination—a visual assessment and a spectrophotometric assay. The response of pollen to the germination media was linear for 16 h. At 6 h after treatment, absorbance at 500 nm was nearly 0.5; consequently, 6 h was chosen to conduct the pollen assays using the spectrophotometer. Both assessment methods differentiated the susceptible (S) and resistant (R) biotypes. Pollen from the susceptible biotype of johnsongrass was strongly inhibited by increasing concentrations of clethodim, with a GR50 of 25.8 ± 0.6 (SE) µM and GR50 of 16.4 ± 1.7 (SE) µM clethodim by visual assessment and spectrophotometric assessment, respectively. Minimum R/S values were > 3.9 by visual assessment and > 6.1 by spectrophotometric assessment. ACCase target-site resistance is expressed in johnsongrass pollen.

Keywords

johnsongrass, Sorghum halepense (L.) Pers. SORHAPollen assays
Type
Research
Information
Weed Technology , Volume 21 , Issue 2 , June 2007 , pp. 384 - 388
Copyright
Copyright © Weed Science Society of America 

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