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A Complex Coacervate Formulation for Delivery of Colletotrichum truncatum 00-003B1

Published online by Cambridge University Press:  20 January 2017

Russell K. Hynes*
Affiliation:
Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
Paulos B. Chumala
Affiliation:
Department of Chemistry, University of Saskatchewan, Saskatoon, SK S7N 5C9, Canada
Daniel Hupka
Affiliation:
Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
Gary Peng
Affiliation:
Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, SK S7N 0X2, Canada
*
Corresponding author's E-mail: [email protected].

Abstract

A complex coacervate formulation was developed for Colletotrichum truncatum 00-003B1 (Ct), a bioherbicidal fungus against scentless chamomile, and tested in the greenhouse. A two-step process was developed to formulate Ct conidia: (1) invert emulsion preparation—emulsify an aqueous suspension of Ct conidia in nonrefined vegetable oil with the aid of a surfactant, and (2) encapsulate the Ct conidia invert emulsion by complex coacervation. Formulation ingredients, including nonrefined vegetable oils, surfactants, proteins, and carbohydrates, and formulation-processing parameters, including mixing speed and the amount of oil added to invert emulsions, were examined for maximum retention of Ct conidia in the formulation. Most formulation ingredients considered and tested in this study were compatible with Ct, with no significant reduction in conidial germination and mycelial growth. The surfactant soya lecithin promoted the greatest retention of Ct conidia (88%) in the invert emulsion, followed by sorbitan monooleate (82%), glycerol monooleate (70%), and sorbitan trioleate (55%). Optimal retention of Ct conidia in the invert emulsion was observed with a water : oil ratio of 1 : 1.8 to 1 : 3.7, and an overhead paddle stirring speed of 300 rpm when preparing the emulsion. Complex coacervate wall ingredients of 1% gelatin and 2% gum arabic were most effective for Ct conidia retention. In greenhouse studies, scentless chamomile disease rating, following a 24-h dew period, was higher on plants sprayed with the Ct conidia complex coacervate formulation than on plants with Ct conidia suspended in 0.1% Tween 80.

Una fórmula compleja múltiple fue desarrollada para Collectotrichum truncatun 00-003B1 (Ct), un herbicida biológico a base de hongos contra la manzanilla sin olor (falsa manzanilla), Tripleurospermun perforatum (Matricaria perforata), y fue probada bajo condiciones de invernadero y de campo. Se llevó a cabo un proceso de dos pasos para formular Ct conidia: i) Invertir la preparación de la emulsión –emulsificar una suspensión acuosa de Ct conidia en un aceite vegetal no refinado con la ayuda de un surfactante y ii) encapsular la emulsión invertida de Ct conidia por coacervación compleja. Los ingredientes de la fórmula, que incluían aceites vegetales no refinados, surfactantes, proteínas y carbohidratos y así como otros parámetros tales como: velocidad mixta y la cantidad de aceite adicionado para invertir emulsiones, fueron examinados para determinar la máxima retención de Ct conidia en la misma. La mayoría de los ingredientes de la fórmula considerados y probados en este estudio, fueron compatibles con Ct, con un impacto no significativo observado en la germinación conidial y en el crecimiento micelial. Los surfactantes de lecitina de soya proporcionaron la mejor retención de Ct conidia (el 88% de la emulsión invertida) seguida por sorbitán monooleato (82%), glicerol monooleato (70%), y sorbitán trioleate (55%). La retención optima de Ct conidia en la emulsión invertida fue observada con una relación de agua a aceite de 1:1.8 −1:3.7 y una velocidad de mezcla de 300 rpm cuando la emulsión se preparaba. Los ingredientes gelatina/chicle árabe, 1 y 2% respectivamente, fueron más efectivos para la retención de Ct. Conidia. La eficacia del control de malezas de la fórmula fue evaluada en la manzanilla sin olor en condiciones de invernadero y de campo. En los estudios que corresponden al invernadero, el porcentaje de enfermedad observado en la manzanilla sin olor después de un período de rocío de 24 horas fue más alto en las plantas donde se roció la fórmula de coacervación compleja Ct conidia, en comparación con Ct conidia suspendida en 0.1% Tween 80. En los estudios de campo, un efecto adicional fue observado en la manzanilla sin olor, en el cuál se redujo el peso de la planta seca, NS P=0 al metribuzin, aplicando una dosis dividida de la fórmula de coacervación compleja de Ct y el herbicida metribuzin (Sencor ®).

Type
Symposium
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Copyright her majesty the queen in right of Canada as represented by Agriculture & Agri-Food Canada

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