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A Seedling Assay to Screen Aryloxyphenoxypropionic Acid and Cyclohexanedione Resistance in Johnsongrass (Sorghum halepense)

Published online by Cambridge University Press:  20 January 2017

Ian C. Burke ,
Walter E. Thomas ,
James D. Burton ,
Janet F. Spears  and
John W. Wilcut
Ian C. Burke
Affiliation:
Crop and Soil Science Department, Washington State University, Pullman, WA 99164-6420
Walter E. Thomas
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
James D. Burton
Affiliation:
Horticulture Department, North Carolina State University, Raleigh, NC 27695-7609
Janet F. Spears
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
John W. Wilcut*
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
*
Corresponding author's E-mail: [email protected].
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Abstract

A seedling bioassay was developed for the rapid diagnosis of resistance to clethodim and fluazifop-P in johnsongrass. The assay was based on differences in the coleoptile length of susceptible (S) and resistant (R) seedlings exposed to clethodim and fluazifop-P in petri dishes for 5 d. Bioassay concentrations of 0.09 mg/L clethodim and 0.18 mg/L fluazifop-P were chosen as discriminant based on rate responses of each biotype to increasing herbicide dose. At 5 d after treatment (DAT), the amounts of clethodim required to reduce coleoptile length by 50% (GR50) for the R and S seedlings were 462.5 and 24.8 mg/L, respectively, resulting in an R:S ratio of 18.7. The fluazifop GR50 values for the R and S seedlings were 618.7 and 17.5 mg/L, respectively, resulting in a R:S ratio of 35.4.

Keywords

Clethodim, fluazifop-P, johnsongrass, Sorghum halepense (L.) Pers. #3 SORHAACCase inhibitorsherbicide resistanceACCase, acetyl coenzyme-A carboxylaseAOPP, aryloxyphenoxypropionateCHD, cyclohexanedione
Type
Research
Information
Weed Technology , Volume 20 , Issue 4 , December 2006 , pp. 950 - 955
Copyright
Copyright © Weed Science Society of America 

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