Hostname: page-component-586b7cd67f-tf8b9 Total loading time: 0 Render date: 2024-11-24T11:34:56.188Z Has data issue: false hasContentIssue false
  • English
  • Français

Metabolic profiling of glyphosate-resistant sourgrass (Digitaria insularis)

Published online by Cambridge University Press:  05 March 2020

Tiago Gazola Open the ORCID record for Tiago Gazola [Opens in a new window] ,
Leandro Bianchi ,
Márcio Furriela Dias Open the ORCID record for Márcio Furriela Dias [Opens in a new window] ,
Caio A. Carbonari Open the ORCID record for Caio A. Carbonari [Opens in a new window]  and
Edivaldo D. Velini Open the ORCID record for Edivaldo D. Velini [Opens in a new window]
Tiago Gazola
Affiliation:
Ph.D. Student, Department of Plant Protection, Paulista State University, São Paulo, Brazil
Leandro Bianchi
Affiliation:
Ph.D. Student, Department of Plant Protection, Paulista State University, São Paulo, Brazil
Márcio Furriela Dias
Affiliation:
Ph.D. Student, Department of Plant Protection, Paulista State University, São Paulo, Brazil
Caio A. Carbonari*
Affiliation:
Associate Professor, Department of Production and Plant Breeding, Paulista State University, São Paulo, Brazil
Edivaldo D. Velini
Affiliation:
Professor, Department of Production and Plant Breeding, Paulista State University, São Paulo, Brazil
*
Author for correspondence: Caio Antonio Carbonari, Department of Production and Plant Breeding, Paulista State University, University Avenue 3780, Botucatu, São Paulo, Brazil. Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Putative glyphosate-resistant sourgrass was collected to determine its resistance level and to evaluate its metabolic profile after resistance. Although accumulation of shikimic acid is known to occur in glyphosate-susceptible populations, differences in the ability of resistant (R) and susceptible (S) biotypes to accumulate quinic acid, salicylic acid, and aminomethylphosphonic acid (AMPA) have been studied to a lesser extent. Our objective was to confirm glyphosate resistance in sourgrass and to understand the metabolic profile of these plants in response to the herbicide. Greenhouse experiments were carried out from January 2016 to June 2018. There were no significant differences in glyphosate translocation in the plants. No metabolism of glyphosate to AMPA was observed; therefore, metabolism of glyphosate to AMPA is not a mechanism in R biotypes. S biotypes showed higher concentrations of shikimic acid and quinic acid before glyphosate and accumulated less of both secondary acids in treated leaves 72 h after glyphosate application. Resistant biotypes showed higher concentrations of salicylic acid before glyphosate application.

Keywords

AMPAEPSPSherbicide resistancequinic acidsalicylic acidshikimic acidGlyphosatesourgrass, Digitaria insularis (L.) Mez ex Ekman
Type
Research Article
Information
Weed Technology , Volume 34 , Issue 5 , October 2020 , pp. 748 - 755
Copyright
© Weed Science Society of America, 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Associate Editor: Scott McElroy, Auburn University

References

Adegas, FS, Vargas, L, Gazziero, DLP, Karam, D, Silva, AF, Agostinetto, D (2017) Impacto econômico da resistência de plantas daninhas a herbicidas no Brasil. Embrapa Soja Circular técnica nº. 132. Londrina, Brazil: Embrapa Soja. 12 pGoogle Scholar
Araújo, R (2008) Validação de metodologia de análise de pesticidas agrícolas em águas por LC/MS. Masters Thesis in Chemical Engineering. Porto, PT: University of Porto, Faculty of Engineering. 70 pGoogle Scholar
Bentley, R, Haslam, E (1990) The shikimate pathway: a metabolic tree with many branches. Crit Rev Biochem Mol Biol 25:307–384 CrossRefGoogle Scholar
Carbonari, CA, Gomes, GLGC, Velini, ED, Machado, RF, Simões, PS, Macedo, GC (2014) Glyphosate effects on sugarcane metabolism and growth. Am J Plant Sci 5:3585–3593 CrossRefGoogle Scholar
Carvalho, LB, Cruz-Hipolito, H, González-Torralva, F, Alves, PLCA, Christoffoleti, PJ, De Prado, R (2011) Detection of sourgrass (Digitaria insularis) biotypes resistant to glyphosate in Brazil. Weed Sci 59:171–176 CrossRefGoogle Scholar
Carvalho, LB, Alves, PLCA, González-Torralva, F, Cruz-Hipolito, E, Rojano-Delgado, AM, De Prado, R, Gil-Humanes, J, Barro, F, De Castro, MDL (2012) Pool of resistance mechanisms to glyphosate in Digitaria insularis. J Agric Food Chem 60:615–622 CrossRefGoogle ScholarPubMed
Costa, FR, Carvalho, LB, Cruz-Hipolito, HE, Alves, PLCA, De Prado, R (2014) The intensity of on-target site mechanisms influences the level of resistance of sourgrass to glyphosate. Commun Plant Sci 4:11–17 Google Scholar
Duke, SO, Powles, SB (2008) Glyphosate: a once-in-a-century herbicide. Pest Manag Sci 64:319–325 CrossRefGoogle Scholar
Gaille, C, Kast, P, Haas, D (2002) Salicylate biosynthesis in Pseudomonas aeruginosa: Purification and characterization of PchB, a novel bifunctional enzyme displaying isochorismate pyruvate-lyase and chorismate mutase activies. J Biol Chem 277:21768–21775 CrossRefGoogle Scholar
Galeano, E, Barroso, AAM, Vasconcelos, TS, López-Rubio, A, Albrecht, AJP, Victoria Filho, R, Carrer, H (2016) EPSPS variability, gene expression., and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis . Gen Mol Res 15:2–15 CrossRefGoogle Scholar
Ge, X, d’Avignon, DA, Ackerman, JJH, Sammons, RD (2010) Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag Sci 66:345–348 CrossRefGoogle Scholar
Ge, X, d’Avignon, DA, Ackerman, JJH, Collavo, A, Sattin, M, Ostrander, EL, Hall, EL, Sammons, RD, Preston, C (2012) Vacuolar glyphosate-sequestration correlates with glyphosate resistance in ryegrass (Lolium spp.) from Australia, South America, and Europe: a 31P NMR investigation. J Agric Food Chem 60:1243–1250 CrossRefGoogle ScholarPubMed
Gomes, GLGC, Velini, ED, Carbonari, CA (2015) Extraction and simultaneous determination of glyphosate, AMPA and compounds of the shikimic acid pathway in plants. Planta Daninha 33:295–304 CrossRefGoogle Scholar
Heap, IA (2019) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed: April 27, 2019Google Scholar
Harring, T, Streibig, JC, Huested, S (1998) Accumulation of shikimic acid: A technique for screening glyphosate efficiency. J Agric Food Chem 46:4406–4412 CrossRefGoogle Scholar
Kissmann, KG, Groth, D (1997) Plantas infestantes e nocivas. Tomo I, São Paulo, Basf Brasileira. Pp 675–678Google Scholar
Leuschner, C, Herrmann, KM, Schultz, G (1995) The metabolism of quinate in pea roots: purification and partial characterization of a quinate hydrolyase. Plant Physiol 108:319–325 CrossRefGoogle Scholar
Lopez Ovejero, R, Takano, HK, Nicolai, M, Ferreira, A, Melo, MSC, Cavenaghi, AL, Christoffoleti, PJ, Oliveira, RS Jr (2017) Frequency and dispersal of glyphosate-resistant sourgrass (Digitaria insularis) populations across Brazilian agricultural production areas. Weed Sci 65:285–294 CrossRefGoogle Scholar
Lorenzi, H (2008) Plantas daninhas do Brasil: terrestres, aquáticas, parasitas e tóxicas. 4º Ed. Nova Odessa, SP: Instituto Plantarum. 672 pGoogle Scholar
Machado, AFL, Ferreira, LR, Ferreira, FA, Fialho, CMT, Tuffi Santos, LD, Machado, MS (2006) Análise de crescimento de Digitaria insularis . Planta Daninha: 24:641–647 CrossRefGoogle Scholar
Machado, AFL, Meira, RMS, Ferreira, LR, Ferreira, FA, Tuffi Santos, LD, Fialho, CMT, Machado, MS (2008) Caracterização anatômica de folha, colmo e rizoma de Digitaria insularis . Planta Daninha 26:1–8 CrossRefGoogle Scholar
Martins, JFM, Barroso, AAM, Carvalho, LB, Cesarin, AE, Amaral, CL, Nepomuceno, MP, Desidério, JA, Alves, PLCA (2016) Plant growth and genetic polymorphism in glyphosate-resistant sourgrass (Digitaria insularis L. Fedde). Austr J Crop Sci 10:1466–1473 Google Scholar
Mondo, VHV, Carvalho, SJP, Dias, ACR, Marcos Filho, J (2010) Efeitos da luz e temperatura na germinação de sementes de quatro espécies de plantas daninhas do gênero Digitaria . Rev Bras Sem 32:131–137 CrossRefGoogle Scholar
Nandula, VK, Reddy, KN, Rimando, AM, Duke, SO, Poston, DH (2007) Glyphosate-resistant and susceptible soybean (Glycine max) and canola (Brassica napus) dose response and metabolism relationship with glyphosate. J Agric Food Chem 55:3540–3545 CrossRefGoogle ScholarPubMed
Orcaray, L, Zulet, A, Zabalza, A, Royuela, M (2012) Impairment of carbon metabolism induced by the herbicide glyphosate. J Plant Physiol 169:27–33 CrossRefGoogle Scholar
Orcaray, L, Igal, M, Marino, D, Zabalza, A, Royuela, M (2010) The possible role of quinate in the mode of action of glyphosate and acetolactate synthase inhibitors. Pest Manag Sci 66:262–269 CrossRefGoogle Scholar
Powles, SB, Preston, C (2006) Evolved glyphosate resistance in plants: biochemical and genetic basis of resistance. Weed Technol 20:282–289 CrossRefGoogle Scholar
Popova, L, Pancheva, T, Uzunova, A (1997) Salicylic acid: properties, biosynthesis and physiological role. Bull J Plant Phys 23:85–93 Google Scholar
Reddy, KN, Rimando, AM, Duke, SO (2004) Aminomethylphosphonic acid, a metabolite of glyphosate, causes injury in glyphosate-treated, glyphosate-resistant soybean. J Agric Food Chem 52:5139–5143 CrossRefGoogle Scholar
Ryals, JA, Neuenschwander, UH, Willits, MG, Molina, A, Steiner, HY, Hunt, MD (1996) Systemic acquired resistance. Plant Cell 8:1809–1819 CrossRefGoogle Scholar
Takano, HK, Oliveira, RS Jr, Constantin, J, Mangolim, CA, Machado, MFPS, Bevilaqua, MRR (2018) Spread of glyphosate-resistant sourgrass (Digitaria insularis): Independent selections or merely propagule dissemination? Weed Biol Manag 18:50–60 CrossRefGoogle Scholar
Sociedade Brasileira da Ciência das Plantas Daninhas (1995) Procedimentos para instalação, avaliação e análise de experimentos com herbicidas. Londrina: SBCPD. 42 pGoogle Scholar
Velini, ED, Duke, SO, Trindade, MLB, Meschede, DK, Carbonari, CA (2009) Modo de ação do glyphosate. Pages 113–134 in Velini, ED, Meschede, DK, Carbonari, CA, Trindade, MLB, 1ª ed. Glyphosate. Botucatu: Fepaf Google Scholar
Ward, ER, Uknes, SJ, Willians, SC, Dincher, SS, Wiederhold, DL, Alexander, DC, Ahl-Goy, P, Métraux, JP, Ryals, JA (1991) Coordinate gene activity in response to agents than induce systems acquired resistance. Plant Cell 3:1085–1094 CrossRefGoogle Scholar