Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-18T18:13:20.308Z Has data issue: false hasContentIssue false
  • English
  • Français

Variation in tolerance mechanisms to fluazifop-P-butyl among selected zoysiagrass lines

Published online by Cambridge University Press:  05 April 2019

Wenwen Liu ,
Gregory E. MacDonald ,
J. Bryan Unruh ,
Kevin E. Kenworthy ,
Laurie E. Trenholm  and
Ramon G. Leon Open the ORCID record for Ramon G. Leon [Opens in a new window]
Wenwen Liu
Affiliation:
Graduate Research Assistant, Department of Agronomy, University of Florida, Gainesville, FL, USA
Gregory E. MacDonald
Affiliation:
Professor, Department of Agronomy, University of Florida, Gainesville, FL, USA
J. Bryan Unruh
Affiliation:
Professor, West Florida Research and Education Center, University of Florida, Jay, FL, USA
Kevin E. Kenworthy
Affiliation:
Professor, Department of Agronomy, University of Florida, Gainesville, FL, USA
Laurie E. Trenholm
Affiliation:
Professor, Department of Environmental Horticulture, University of Florida, Gainesville, FL, USA
Ramon G. Leon*
Affiliation:
Assistant Professor, Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, USA
*
Author for correspondence: Ramon G. Leon, Email: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Breeding herbicide tolerance into new cultivars can improve safety and weed control in turfgrass systems. The sensitivity to fluazifop-P-butyl of 27 zoysiagrass (Zoysia spp.) lines was screened under greenhouse conditions to identify potential tolerant germplasm for breeding programs. The herbicide rate that caused 50% biomass reduction (GR50) and the rate that caused 50% injury (ID50) were calculated to select the three most-tolerant and the five most-susceptible lines for studying the physiological mechanisms responsible for fluazifop-P-butyl tolerance. The differences in GR50 and ID50 between susceptible and tolerant lines ranged from 4-fold to more than 10-fold. Cytochrome P450–mediated metabolism was not detected in fluazifop-P-butyl–tolerant lines. Sequencing of the ACCase gene confirmed that none of the seven previously reported mutations conferring resistance to acetyl-CoA carboxylase (ACCase)-inhibiting herbicides in other species were present in any of the tolerant or susceptible zoysiagrass lines studied. An Ala-2073-Thr substitution was identified in two tolerant lines, but this mutation did not completely explain the tolerant phenotype. No clear differences in absorption and translocation rates of 14C-radiolabeled fluazifop-P-butyl were observed among most lines, with the exception of a susceptible line that exhibited greater translocation than two of the tolerant lines. Metabolite profiles did not differ between tolerant and susceptible lines. Our results suggest that the diversity in tolerance to fluazifop-P-butyl in zoysiagrass germplasm is most likely the result of a combination of different, minor, additive non–target site mechanisms such as translocation rate and compartmentation after absorption.

Keywords

Franck E. Dayan, Colorado State UniversityACCaseAcetyl-coenzyme A carboxylasegenetic diversitygraminicideherbicide resistance
Type
Research Article
Information
Weed Science , Volume 67 , Issue 3 , May 2019 , pp. 288 - 295
Copyright
© Weed Science Society of America, 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anonymous (2009) Fusilade® II product label. Syngenta Crop Protection. Greensboro, NC: Syngenta. 37 p.Google Scholar
Baerg, RJ, Barrett, M, Polge, ND (1996) Insecticide and insecticide metabolite interactions with cytochrome P450 mediated activities in maize. Pestic Biochem Physiol 55:10–20CrossRefGoogle ScholarPubMed
Boydston, RA (1992) Drought stress reduces fluazifop-P activity on green foxtail (Setaria viridis). Weed Sci 40:20–24Google Scholar
Carr, JE, Davies, LG, Cobb, AH, Pallet, KE (1985) The metabolic activity of fluazifop acid in excised apical meristem sections. Pages 155–162 in Proceedings of the British Crop Protection Conference, Weeds. Volume 1. Brighton, UK: British Crop Protection CouncilGoogle Scholar
Délye, C, Matejicek, A, Gasquez, J (2002) PCR-based detection of resistance to acetyl-CoA carboxylase-inhibiting herbicides in black-grass (Alopecurus myosuroides Huds) and ryegrass (Lolium rigidum Gaud). Pest Manag Sci 58:474–478CrossRefGoogle Scholar
Délye, C, Zhang, XQ, Michel, S, Matejicek, A, Powles, SB (2005) Molecular bases for sensitivity to acetyl-coenzyme a carboxylase inhibitors in black-grass. Plant Physiol 137:794–806CrossRefGoogle ScholarPubMed
Devine, MD (1997) Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review. Pestic Sci 51:259–2643.0.CO;2-S>CrossRefGoogle Scholar
Devine, MD, Hall, LM (1990) Implications of sucrose transport mechanisms for the translocation of herbicides. Weed Sci 38:299–304Google Scholar
Ferhatoglu, Y, Avdiushko, S, Barrett, M (2005) The basis for the safening of clomazone by phorate insecticide in cotton and inhibitors of cytochrome P450s. Pestic Biochem Physiol 81:59–70CrossRefGoogle Scholar
Goggin, DE, Kaur, P, Owen, MJ,Powles, SB (2018) 2,4-D and dicamba resistance mechanisms in wild radish: subtle, complex and population specific? Ann Bot 122:627–640CrossRefGoogle ScholarPubMed
Haughn, GW, Somerville, C (1986) Sulfonylurea-resistant mutants of Arabidopsis thaliana. Mol Gen Genet 204:430–434CrossRefGoogle Scholar
Heap, IM (2018) The International Survey of Herbicide-Resistant Weeds. http://www.weedscience.org. Accessed: August 23, 2018CrossRefGoogle Scholar
Hendley, P, Dicks, JW, Monaco, TJ, Slyfield, SM, Tummon, OJ, Barrett, JC (1985) Translocation and metabolism of pyrin-dinyloxy-p henoxypropionate herbicides in rhizomatous quackgrass (Agropyron repens). Weed Sci 33:11–24Google Scholar
Hidayat, I, Preston, C (2001) Cross-resistance to imazethapyr in a fluazifop-P-butyl-resistant population of Digitaria sanguinalis. Pestic Biochem Physiol 71:190–195CrossRefGoogle Scholar
Hunter, JH (1995) Effect of bud vs rosette growth stage on translocation of 14C-glyphosate in Canada thistle (Cirsium arvense). Weed Sci 43:347–351Google Scholar
Jander, G, Baerson, SR, Hudak, JA, Gonzalez, KA, Gruys, KJ, Last, RL (2003) Ethylmethanesulfonate saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance. Plant Physiol 131:139–146CrossRefGoogle ScholarPubMed
Johnson, BJ (1992) Common bermudagrass (Cynodon dactylon) suppression in Zoysia spp. with herbicides. Weed Technol 6:813–819CrossRefGoogle Scholar
Kimball, JA, Zuleta, MC, Kenworthy, KE, Lehman, VG, Milla-Lewis, S (2012) Assessment of genetic diversity in Zoysia species using amplified fragment length polymorphism. Crop Sci 52:360–370CrossRefGoogle Scholar
Leon, RG, Unruh, JB, Brecke, BJ, Kenworthy, KE (2014) Characterization of fluazifop-P-butyl tolerance in zoysiagrass cultivars. Weed Technol 28:385–394CrossRefGoogle Scholar
Liu, WJ, Harrison, DK, Chalupska, D, Gornicki, P, O’Donnell, CC, Adkins, SW, Haselkorn, R, Williams, RR (2007) Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc Natl Acad Sci USA 104:3627–3632CrossRefGoogle ScholarPubMed
Liu, W, MacDonald, GE, Unruh, JB, Kenworthy, KE, Trenholm, LE, Leon, RG (2017) Application timing affects tolerance of zoysiagrass to fluazifop-P-butyl and safening effect of triclopyr. Abstract 271-11 in ASA-CSSA-SSSA Annual Meeting Abstracts. Tampa, FL, October 2017.Google Scholar
McElroy, JS, Breeden, GK (2006) Triclopyr safens the use of fluazifop and fenoxaprop on zoysiagrass while maintaining bermudagrass suppression. Appl Turfgrass Sci 3, 10.1094/ATS-2006-0502-01-RSCrossRefGoogle Scholar
Moore, KA, Zuleta, MC, Patton, AJ, Schwartz, BM, Aranaz, G, Milla-Lewis, SR (2017) SSR allelic diversity shifts in zoysiagrass (Zoysia spp.) cultivar released from 1910 to 2016. Crop Sci 57:S1–S12CrossRefGoogle Scholar
Patton, A, Schwartz, BM, Kenworthy, KE (2017a) Zoysiagrass (Zoysia spp.) history, utilization, and improvement in the United States: a review. Crop Sci 57:S37–S72CrossRefGoogle Scholar
Patton, AJ, Trappe, JM, Doroh, MC, McElroy, JS (2017b) Evaluation of herbicides and their tank-mixes for suppression of bermudagrass in zoysiagrass. Int Turf Soc Res J 13:716–722Google Scholar
Petit, C, Duhieu, B, Boucansaud, K, Délye, C (2010) Complex genetic control of non-target-site-based resistance to herbicides inhibiting acetyl-coenzyme A carboxylase and acetolactate-synthase in Alopecurus myosuroides Huds. Plant Sci 178:501–509CrossRefGoogle Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347CrossRefGoogle ScholarPubMed
Preston, C, Tardif, FJ, Christopher, JT, Powles, SB (1996) Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic Biochem Physiol 54:123–134CrossRefGoogle Scholar
San Cha, T, Najihah, MG, Sahid, IB, Chuah, TS (2014) Molecular basis for resistance to ACCase-inhibiting fluazifop in Eleusine indica from Malaysia. Pestic Biochem Physiol 111:7–13Google Scholar
Shah, DM, Horsch, RB, Klee, KJ, Kishore, GM, Winter, JA, Tumer, NE, Hironaka, CM, Sander, PR, Gasser, CS, Aykent, S, Siegel, NR, Roger, SG, Fraley, RT (1986) Engineering herbicide tolerance in transgenic plants. Science 233:748–481CrossRefGoogle ScholarPubMed
Souza Machado, V, Bandeen, JD, Stephenson, GR, Lavigne, P (1978) Uniparental inheritance of chloroplast atrazine tolerance in Brassica campestris. Can J Plant Sci 58:977–981CrossRefGoogle Scholar
Sun, Y, Zhang, X, Wu, C, He, Y, Ma, Y, Hou, H, Guo, X, Du, W, Zhao, Y, Xia, L (2016) Engineering herbicide-resistant plants through CRISPR/Cas9-mediated homologous recombination of acetolactate synthase. Mol Plant 9:628–631CrossRefGoogle ScholarPubMed
Tang, W, Zhou, F, Chen, J, Zhou, X (2014) Resistance to ACCase-inhibiting herbicides in an Asia minor bluegrass (Polypogon fugax) population in China. Pestic Biochem Physiol 108:16–20CrossRefGoogle Scholar
Tardif, FJ, Hokum, JAM, Powles, SB (1993) Occurrence of a herbicide-resistant acetyl-coenzyme A carboxylase mutant in annual ryegrass (Lolium rigidum) selected by sethoxydim. Planta 190:176–181CrossRefGoogle Scholar
Tate, TM (2012) Characterization of Acetyl Coenzyme A Inhibitor Resistance in Turfgrass and Grassy Weeds. Ph.D dissertation. University of Georgia, Griffin. 56 pGoogle Scholar
Vila-Aiub, MM, Neve, P, Powles, SB (2005) Resistance cost of a cytochrome P450 herbicide metabolism mechanism but not an ACCase target site mutation in a multiple resistant Lolium rigidum population. New Phytol 167:787–796CrossRefGoogle ScholarPubMed
Werck-Reichhart, D, Hehn, A, Didierjean, L (2000) Cytochromes P450 for engineering herbicide tolerance. Trends Plant Sci 5:116–123CrossRefGoogle ScholarPubMed
Yu, Q, Collavo, A, Zheng, MQ, Owen, M, Sattin, M, Powles, SB (2007) Diversity of acetyl-coenzyme a carboxylase mutation in resistant Lolium populations: evaluation using clethodim. Plant Physiol 145:547–558CrossRefGoogle ScholarPubMed