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Using Protein Electrophoresis to Investigate the Phylogeny of Velvetleaf (Abutilon theophrasti)

Published online by Cambridge University Press:  12 June 2017

Steven J. Stegink  and
Neal R. Spencer
Steven J. Stegink
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., Stoneville, MS 38776
Neal R. Spencer
Affiliation:
South. Weed Sci. Lab., Agric. Res. Serv., U.S. Dep. Agric., Stoneville, MS 38776
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Abstract

Native Polyacrylamide gel electrophoresis (PAGE) and enzyme activity staining were used to identify possible progenitor species of velvetleaf (Abutilon theophrasti Medic. # ABUTH). Multiple forms of superoxide dismutase activity were observed in each of the plants surveyed. Three enzyme forms were common to all the species and bio types while one form was different in the velvetleaf biotypes compared to the other species. Multiple forms of peroxidase activity were also detected. The three velvetleaf biotypes possessed identical enzyme forms with minimal similarity to peroxidase forms found in the other species. Multiple forms of esterase activity separated as two nonoverlapping groups. A slowly migrating group was observed in all the velvetleaf biotypes and a more rapidly migrating group characterized the remaining Abutilon species. The results of this study indicated that the progenitors of velvetleaf were not among the species surveyed and suggested that the progenitors may no longer be extant.

Keywords

ChemotaxonomyAbutilon bidentatumAbutilon fruticosumAbutilon graveolensAbutilon guineenseAbutilon hirtumAbutilon indicumAbutilon mauritianumAbutilon muticumAbutilon pannosumAbutilon umb elliflorum
Type
Weed Biology and Ecology
Information
Weed Science , Volume 36 , Issue 2 , March 1988 , pp. 172 - 175
Copyright
Copyright © 1988 by the Weed Science Society of America 

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References

Literature Cited

1. Baker, E. G. 1893. Synopsis of Genera and Species of Malveae. J. Bot. 31:214.Google Scholar
2. Waalkes, Borssum, van, J. 1966. Malesian Malvaceae Revised. Blumea 14(1):170.Google Scholar
3. Bradford, M. M. 1966. A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding. Anal. Biochem. 72:248–254.Google Scholar
4. Cherry, J. P., Katterman, F., and Endrizzi, J. 1972. Seed esterases, leucine aminopeptidases, and catalases of species of the genus Gossypium . Theor. Appl. Gen. 42:218–226.CrossRefGoogle ScholarPubMed
5. Darmency, H. and Gasquez, J. 1983. Esterase polymorphism and growth form differentiation in Poa annua L. New Phytol. 95:289–297.CrossRefGoogle Scholar
6. de Wet, J.M.J. 1971. Polyploidy and evolution of plants. Taxon. 20:29–35.Google Scholar
7. Gottlieb, L. D. 1973. Genetic differentiation, sympatric speciation, and the origin of a diploid species of Stephanomeria . Am J. Bot. 60:545–553.Google Scholar
8. Gottlieb, L. D. 1977. Electrophoretic evidence and plant systematics. Ann. Missouri Bot. Gard. 64:161–180.Google Scholar
9. Harris, H. and Hopkinson, D. A. 1976. 3.1.1.1 -Esterase, in Harris, H. and Hopkinson, D. A., eds. Handbook of Enzyme Electrophoresis in Human Genetics. American Elsevier Publishing Co., New York.Google Scholar
10. Kirkpatrick, K. J., Decker, D. S., and Wilson, H. D. 1985. Allozyme differentiation in the Cucurbita pepo complex: C. pepo var. medullosa vs. C. texana . Econ. Bot. 39:289–299.Google Scholar
11. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685.Google Scholar
12. Love, A. 1982. IOPB chromosome number report LXXVII. Taxon. 31:761–777.Google Scholar
13. Roose, M. L. and Gottlieb, L. D. 1976. Genetic and biochemical consequences of polyploidy in Tragopogon . Evolution 30: 818–830.Google Scholar
14. Spencer, N. R. 1984. Velvetleaf, Abutilon theophrasti (Malvaceae), history and economic impact in the United States. Econ. Bot. 38:407–416.Google Scholar
15. Spencer, N. R. 1985. Biological Control of Velvetleaf: Foreign Exploration Phase. Proc. South. Weed Sci. Soc. 38:363–364.Google Scholar
16. Spencer, N. R. and Sankaran, T. 1986. Prospects for the Biological Control of Velvetleaf, Abutilon theophrasti . Proc. VI International Symposium on the Biological Control of Weeds. Vancouver, Canada.Google Scholar
17. Stebbins, G. L. 1947. Types of polyploids: Their classification and significance. Adv. Genet. 1:403–429.Google Scholar
18. Valiulis, D. 1986. Velvetleaf. Agrichem. Age 30(2):6–7.Google Scholar
19. Vallejos, E. 1983. Enzyme activity staining. Pages 469–516 in Tanksley, S. D. and Orton, T. J., eds. Isozymes in Plant Genetics and Breeding, Part A. Elsevier Science Publishers, Amsterdam.Google Scholar
20. Werth, C. R., Guttman, S. I., and Eshbaugh, W. H. 1985. Electrophoretic evidence of reticulate evolution in the Appalachian Asplenium complex, Syst. Bot. 10:184–192.Google Scholar
21. Werth, C. R., Guttman, S. I., and Eshbaugh, W. H. 1985. Recurring origins of allopolyploid species in Asplenium . Science 228:731–733.CrossRefGoogle ScholarPubMed