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Uptake, Translocation, and Metabolism in Alfalfa (Medicago sativa) Selected for Enhanced Tolerance to Terbacil

Published online by Cambridge University Press:  12 June 2017

Michael. P Anderson ,
Curtis Bensch ,
Jimmy F. Stritzke  and
John L. Caddel
Michael. P Anderson
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
Curtis Bensch
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
Jimmy F. Stritzke
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
John L. Caddel
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078
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Abstract

Terbacil tolerance was investigated in field-selected alfalfa strain ‘OK 182.’ Growth response studies indicated a 78% increase in tolerance over the unselected parental cultivar ‘Cimarron.’ Photosynthetic electron transport was inhibited similarly in both cultivars when isolated thylakoids were exposed to a wide range of terbacil concentration indicating that tolerance was not due to reduced sensitivity at the site-of-action. ‘OK 182’ took up an average of 22% less 14C-terbacil over the 6-d labeling period. A similar proportion of total radiolabel was translocated in both strains indicating that differential translocation was not a factor in the tolerance mechanism. Once in the leaves, terbacil was rapidly metabolized to a glucosidic conjugate by both strains. Terbacil and total concentration of metabolites in leaf tissues were 17% and 33% lower in ‘OK 182’ than in ‘Cimarron,’ respectively. Enhanced tolerance to terbacil in ‘OK 182’ was attributed to decreased terbacil uptake.

Keywords

Terbacil, 5-chloro-3-(1, 1-dimethylethyl)-6-methyl-2,4-(1H,3H)-pyrimidinedionealfalfa, Medicago sativa L. #3 MEDSABreedingherbicide toleranceforage crop
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 43 , Issue 3 , September 1995 , pp. 365 - 369
Copyright
Copyright © 1995 by the Weed Science Society of America 

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