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Transformation of Alachlor by Microbial Communities

Published online by Cambridge University Press:  12 June 2017

Hone L. Sun ,
Thomas J. Sheets  and
Frederick T. Corbin
Hone L. Sun
Affiliation:
Toxicol., North Carolina State Univ., Raleigh, NC 27695-7609
Thomas J. Sheets
Affiliation:
Dep's. Toxicol., North Carolina State Univ., Raleigh, NC 27695-7609
Frederick T. Corbin
Affiliation:
Crop Sci., North Carolina State Univ., Raleigh, NC 27695-7609
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Abstract

A mixed microbial culture able to transform alachlor at a concentration of 50 μg ml-1 was obtained from alachlor-treated soil after an enrichment period of 84 days. The microbial community was composed of seven strains of bacteria. No single isolate was able to utilize alachlor as a sole source of carbon. There was no alachlor left in the enriched culture after a 14-day incubation, but only 12% of the 14C-ring-labeled alachlor was converted to 14CO2 through ring cleavage during 14 days in the basal medium amended with alachlor as a sole carbon source. The presence of sucrose as an alternative carbon source decreased the mineralization potential of the enriched culture, but sucrose increased the mineralizing ability of a three-member mixed culture. Thin-layer chromatographic analysis showed that there were four unidentified metabolites of alachlor produced by the enriched culture. Sucrose decreased the amount of two of the four metabolites. The absence of a noticeable decline in radioactivity beyond the initial 12% suggested that the side chain of alachlor was utilized as carbon source by the enriched culture. Little difference in radioactivity between growth medium and cell-free supernatant of the growth medium suggested that the carbon in the ring was not incorporated into the cells of the degrading microorganisms.

Keywords

Alachlor2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamideDegradation of alachlormetabolism of alachloreffects of sucrosemicrobial decomposition
Type
Soil, Air, and Water
Information
Weed Science , Volume 38 , Issue 4-5 , September 1990 , pp. 416 - 420
Copyright
Copyright © 1990 by the Weed Science Society of America 

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