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Thermal dependence of bioengineered glufosinate tolerance in cotton

Published online by Cambridge University Press:  20 January 2017

James R. Mahan ,
Peter A. Dotray ,
Ginger G. Light  and
Kristy R. Dawson
Peter A. Dotray
Affiliation:
Department of Plant and Soil Science, Texas Tech University, Box 42122, Lubbock, TX 79409-2122
Ginger G. Light
Affiliation:
RR 1, Box 109, Petersburg, TX 79250
Kristy R. Dawson
Affiliation:
Honors College, 103 McClellan Hall, Box 41017, Texas Tech University, Lubbock, TX 79409-1017
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Abstract

Tolerance to glufosinate has been bioengineered into cotton through the expression of a gene encoding the enzyme phosphinothricin acetyl transferase (PAT). Studies were conducted to determine thermal limitations on herbicide efficacy in bioengineered cotton. The 50% inhibition (I50) of glufosinate of the target-site enzyme glutamine synthetase was thermally dependent with the lowest values between 25 and 35 C. Larger values of I50 were measured above and below the 25 to 35 C range. The apparent Michaelis constant KM of the enzyme PAT was relatively stable from 15 to 30 C and increased more rapidly from 30 to 45 C. The two components in combination suggest the aggregate tolerance to glufosinate would not be thermally limited between 15 and 45 C. The thermal dependence of the aggregate tolerance in cotton suggests that glufosinate would not damage the crop over a range of temperatures. This prediction is in agreement with the results of field studies carried out over a number of years, which showed the glufosinate-tolerant cotton to be undamaged by glufosinate over a wide range of temperatures.

Keywords

Glufosinatecotton, Gossypium hirsutum L. ‘SeedCo 9023’, ‘DeltaPine 458’Glutamine synthetaseMichaelis-Menten constantphosphinothricin acetyl transferase
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 54 , Issue 1 , February 2006 , pp. 1 - 5
Copyright
Copyright © Weed Science Society of America 

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