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Target-site mutation and enhanced metabolism confer resistance to thifensulfuron-methyl in a multiple-resistant redroot pigweed (Amaranthus retroflexus) population

Published online by Cambridge University Press:  14 December 2020

Yi Cao ,
Shouhui Wei ,
Hongjuan Huang ,
Wenyu Li ,
Chaoxian Zhang  and
Zhaofeng Huang Open the ORCID record for Zhaofeng Huang [Opens in a new window]
Yi Cao
Affiliation:
Master’s Degree Student, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Shouhui Wei
Affiliation:
Associate Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Hongjuan Huang
Affiliation:
Associate Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Wenyu Li
Affiliation:
Research Assistant, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Chaoxian Zhang
Affiliation:
Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
Zhaofeng Huang*
Affiliation:
Associate Professor, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing, China
*
Author for correspondence: Zhaofeng Huang, Institute of Plant Protection (IPP), Chinese Academy of Agricultural Sciences (CAAS), Beijing100193, China. (Email: [email protected])
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Abstract

Redroot pigweed (Amaranthus retroflexus L.) is a troublesome dicot weed species widely distributed across China. A population of A. retroflexus that survived the recommended label rate of thifensulfuron-methyl was collected from the main soybean [Glycine max (L.) Merr.] production area in China. Whole-plant dose–response assays indicated that the resistant (R) population was highly resistant (61.80-fold) to thifensulfuron-methyl compared with the susceptible (S1 and S2) populations. In vitro acetolactate synthase (ALS) activity experiments showed that the thifensulfuron-methyl I50 value for the R population was 40.17 times higher than that for the S1 population. A preliminary malathion treatment study indicated that the R population might have cytochrome P450–mediated metabolic resistance. The R population exhibited a high level of cross-resistance to representative ALS herbicides (imazethapyr, flumetsulam, and bispyribac-sodium) and multiple resistance to the commonly used protoporphyrinogen oxidase (PPO)-inhibiting herbicides lactofen and fomesafen. Two common mutations, Trp-574-Leu in ALS and Arg-128-Gly in PPO2, were identified within the R population. This study identified possible enhanced metabolism of thifensulfuron-methyl coexisting with target-site mutations in both ALS and PPO2 in a multiple-resistant A. retroflexus population.

Keywords

ALS activitymetabolismmultiple resistanceredroot amaranth
Type
Research Article
Information
Weed Science , Volume 69 , Issue 2 , March 2021 , pp. 161 - 166
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of Weed Science Society of America

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Footnotes

Associate Editor: Christopher Preston, University of Adelaide

References

Ahmad-Hamdani, MS, Yu, Q, Han, H, Cawthray, GR, Wang, SF, Powles, SB (2012) Herbicide resistance endowed by enhanced rates of herbicide metabolism in wild oat (Avena spp.) Weed Sci 61:55–62 CrossRefGoogle Scholar
Chen, JY, Huang, ZF, Zhang, CX, Huang, HJ, Wei, SH, Chen, JC, Wang, X (2015) Molecular basis of resistance to imazethapyr in redroot pigweed (Amaranthus retroflexus L.) populations from China. Pestic Biochem Physiol 124:43–47 CrossRefGoogle ScholarPubMed
Christopher, JT, Preston, C, Powles, SB (1994) Malathion antagonizes metabolism-based chlorsulfuron resistance in Lolium rigidum . Pestic Biochem Physiol 49:172–182 CrossRefGoogle Scholar
Délye, C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade. Pest Manag Sci 69:176–187 CrossRefGoogle Scholar
De Prado, RA, Franco, AR (2004) Cross-resistance and herbicide metabolism in grass weeds in Europe: biochemical and physiological aspects. Weed Sci 52:441–447 CrossRefGoogle Scholar
Devine, MD, Shukla, A (2000) Altered target sites as a mechanism of herbicide resistance. Crop Prot 19:881–889 CrossRefGoogle Scholar
Duggleby, RG, Pang, SS (2000) Acetohydroxyacid synthase. J Biochem Mol Biol 33:1–36 Google Scholar
Duhoux, A, Carrère, S, Duhoux, A, Délye, C (2017) Transcriptional markers enable identification of rye-grass (Lolium sp.) plants with non-target-site-based resistance to herbicides inhibiting acetolactate-synthase. Plant Sci 257:22–36 CrossRefGoogle ScholarPubMed
Durner, J, Gailus, V, Boger, P (1990) New aspects on inhibition of plant acetolactate synthase by chlorsulfuron and imazaquin. Plant Physiol 95:1144–1149 CrossRefGoogle Scholar
Ferguson, GM, Hamill, AS, Tardif, FJ (2001) ALS inhibitor resistance in populations of Powell amaranth and redroot pigweed. Weed Sci 49:448–453 CrossRefGoogle Scholar
Francischini, AC, Constantin, J, Oliveira, RS Jr, Santos, G, Franchini, LHM, Biffe, DF (2014) Resistance of Amaranthus retroflexus to acetolactate synthase inhibitor herbicides in Brazil. Planta Daninha 32:437–446 CrossRefGoogle Scholar
Heap, IM (2020) International Herbicide-Resistant Weed Database. http://www.weedscience.com. Accessed: August 23, 2020Google Scholar
Huang, ZF, Chen, JY, Zhang, CX, Huang, HJ, Wei, SH, Zhou, XX, Chen, JC, Wang, X (2016) Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.). Pestic Biochem Physiol 128:10–15 CrossRefGoogle Scholar
Huang, ZF, Cui, HL, Wang, CY, Wu, T, Zhang, CX, Huang, HJ, Wei, SH (2020) Investigation of resistance mechanism to fomesafen in Amaranthus retroflexus L. Pestic Biochem Physiol 165:104560 CrossRefGoogle ScholarPubMed
Karimmojeni, H, Bazrafshan, AH, Majidi, MM, Torabian, S, Rashidi, B (2014) Effect of maternal nitrogen and drought stress on seed dormancy and germinability of Amaranthus retroflexus. Plant Spec Biol 29:e1–e8 Google Scholar
Li, XJ, Zhang, HJ, Ni, HW (2004) Review on the biological characters and control of redroot pigweed (Amaranthus retroflexus L.). Pesticide Sci Adm 25:13–16. ChineseGoogle Scholar
Ma, R, Kaundun, SS, Tranel, PJ, Riggins, CW, McGinness, DL, Hager, AG, Hawkes, T, McIndoe, E, Riechers, DE (2013) Distinct detoxification mechanisms confer resistance to mesotrione and atrazine in a population of waterhemp. Plant Physiol 163:363–377 CrossRefGoogle Scholar
Mei, Y, Si, C, Liu, MJ, Qiu, LH, Zheng, MQ (2017) Investigation of resistance levels and mechanisms to nicosulfuron conferred by non-target-site mechanisms in large crabgrass (Digitaria sanguinalis L.) from China. Pestic Biochem Physiol 141:84–89 CrossRefGoogle ScholarPubMed
McNaughton, KE, Letarte, J, Lee, EA, Tardif, FJ (2005) Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci 53:17–22 CrossRefGoogle Scholar
Nakka, S, Thompson, CR, Peterson, DE, Jugulam, M (2017) Target site-based and non-target site based resistance to ALS inhibitors in palmer amaranth (Amaranthus palmeri). Weed Sci 65:681–689 CrossRefGoogle Scholar
Patzoldt, WL, Tranel, PJ (2007) Multiple ALS mutations confer herbicide resistance in waterhemp (Amaranthus tuberculatus). Weed Sci 55:421–428 CrossRefGoogle Scholar
Powles, SB, Gaines, TA (2016) Exploring the potential for a regulatory change to encourage diversity in herbicide use. Weed Sci 64:649–654 CrossRefGoogle Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347 CrossRefGoogle ScholarPubMed
Preston, C, Tardif, FJ, Christopher, JT (1996) Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic Biochem Physiol 54: 123–134 CrossRefGoogle Scholar
Scarabel, L, Varotto, S, Sattin, M (2010) A European biotype of Amaranthus retroflexus cross-resistant to ALS inhibitors and response to alternative herbicides. Weed Res 47:527–533 CrossRefGoogle Scholar
Seefeldt, SS, Jensen, JE, Fuerst, EP (1995) Log-Logistic analysis of herbicide dose-response relationships. Weed Technol 9:218–227 CrossRefGoogle Scholar
Sibony, M, Michel, A, Haas, HU, Rubin, B, Hurle, K (2010) Sulfometuron-resistant Amaranthus retroflexus: cross-resistance and molecular basis for resistance to acetolactate synthase-inhibiting herbicides. Weed Res 41:509–522 CrossRefGoogle Scholar
Tranel, PJ, Wright, TR, Heap, IM (2020) Mutations in Herbicide-Resistant Weeds to ALS Inhibitors. http://www.weedscience.com. Accessed: August 23, 2020Google Scholar
Umbarger, HE (1978) Amino acid biosynthesis and its regulation. Annu Rev Biochem 47:533–606 CrossRefGoogle ScholarPubMed
Veldhuis, LJ, Hall, LM, O’Donovan, JT, Dyer, W, Hall, JC (2000) Metabolism-based resistance of a wild mustard (Sinapis arvensis L.) biotype to ethametsulfuron-methyl. J Agric Food Chem 48:2986–2990 CrossRefGoogle ScholarPubMed
Wang, Q, Ge, LA, Zhang, LL, You, LD, Wang, DD, Liu, WT, Wang, JX (2019) A Trp-574-Leu mutation in the acetolactate synthase (ALS) gene of Lithospermum arvense L. confers broad-spectrum resistance to ALS inhibitors. Pestic Biochem Physiol 158:12–17 CrossRefGoogle ScholarPubMed
Yang, Q, Deng, W, Li, X, Yu, Q, Bai, L, Zheng, M (2016) Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.). BMC Genomics 17:551 CrossRefGoogle Scholar
Yasuor, H, Osuna, MD, Ortiz, A, Saldain, NE, Eckert, JW, Fisher, A (2009) Mechanism of resistance to penoxsulam in late watergrass [Echinochloa phyllopogon (Stapf) Koss]. J Agric Food Chem 57:3653–3660 CrossRefGoogle Scholar
Yu, Q, Frisesen, LSS, Zhang, XQ, Powles, SB (2004) Tolerance to acetolactate synthase and acetyl-coenzyme A carboxylase inhibiting herbicides in Vulpia bromoidesis conferred by two co-existing resistance mechanisms. Pestic Biochem Physiol 78:21–30 CrossRefGoogle Scholar
Yu, Q, Powles, S (2014) Metabolism-based herbicide resistance and cross-resistance in crop weeds: a threat to herbicide sustainability and global crop production. Plant Physiol 166:1106–1118 CrossRefGoogle ScholarPubMed