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Survey of glyphosate-, atrazine- and lactofen-resistance mechanisms in Ohio waterhemp (Amaranthus tuberculatus) populations

Published online by Cambridge University Press:  14 March 2019

Brent P. Murphy ,
Alvaro S. Larran ,
Bruce Ackley ,
Mark M. Loux  and
Patrick J. Tranel Open the ORCID record for Patrick J. Tranel [Opens in a new window]
Brent P. Murphy
Affiliation:
Graduate Student, Department of Crop Sciences, University of Illinois, Urbana, IL, USA
Alvaro S. Larran
Affiliation:
Graduate Student, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina
Bruce Ackley
Affiliation:
Extension Program Specialist, Department of Horticulture and Crop Science, Ohio State University, Columbus, OH, USA
Mark M. Loux
Affiliation:
Professor, Department of Horticulture and Crop Science, Ohio State University, Columbus, OH, USA
Patrick J. Tranel*
Affiliation:
Professor, Department of Crop Sciences, University of Illinois, Urbana, IL, USA
*
Author for correspondence: Patrick J. Tranel, Email: [email protected]
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Abstract

Herbicide resistance within key driver weeds, such as common waterhemp [Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif ], constrains available management options for crop production. Routine surveillance for herbicide resistance provides a mechanism to monitor the development and spread of resistant populations over time. Furthermore, the identification and quantification of resistance mechanisms at the population level can provide information that helps growers develop effective management plans. Populations of Amaranthus spp., including A. tuberculatus, redroot pigweed (Amaranthus retroflexus L.), and Palmer amaranth (Amaranthus palmeri S. Watson), were collected from 51 fields in Ohio during the 2016 growing season. Twenty-four A. tuberculatus populations were screened for resistance to the herbicides lactofen, atrazine, and glyphosate. Phenotypically resistant plants were further investigated to determine the frequency of known resistance mechanisms. Resistance to lactofen was infrequently observed throughout the populations, with 8 of 22 populations exhibiting resistant plants. Within those eight resistant populations, the ΔG210 resistance mechanism was observed in 17 of 30 phenotypically resistant plants, and the remainder lacked all known resistance mechanisms. Resistance to atrazine was observed in 12 of 15 populations; however, a target-site resistance mechanism was not observed in these populations. Resistance to glyphosate was observed in all populations. Gene amplification was the predominant glyphosate-resistance mechanism (147 of 322 plants) in the evaluated populations. The Pro-106-Ser mutation was identified in 24 plants, half of which also possessed gene amplification. In this study, molecular screening generally underestimated the phenotypically observed resistance. Continued mechanism discovery and marker development is required for improved detection of herbicide resistance through molecular assays.

Keywords

Muthukumar V. Bagavathiannan, Texas A&M UniversityEPSPS gene amplificationherbicide resistanceresistance mechanismstarget-site resistance
Type
Research Article
Information
Weed Science , Volume 67 , Issue 3 , May 2019 , pp. 296 - 302
Copyright
© Weed Science Society of America, 2019 

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Footnotes

*

These authors contributed equally to this work.

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