Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-05T05:26:26.919Z Has data issue: false hasContentIssue false
  • English
  • Français

Surfactant Effects on Isolated Plant Cells

Published online by Cambridge University Press:  12 June 2017

J. B. St. John ,
P. G. Bartels  and
J. L. Hilton
J. B. St. John
Affiliation:
U.S. Dep. of Agr., Agr. Res. Serv., Agr. Res. Center, Agr. Environ. Qual. Inst., Beltsville, MD 20705
P. G. Bartels
Affiliation:
Dep. of Biol. Sci., University of Arizona, Tucson, AZ 85721
J. L. Hilton
Affiliation:
U.S. Dep. of Agr., Agr. Res. Serv., Agr. Res. Center, Agr. Environ. Qual. Inst., Beltsville, MD 20705
Get access Rights & Permissions [Opens in a new window]

Abstract

Isolated plant cells from leaf tissue of soybean [Glycine max (L.) Merr. ‘Harosoy’] and wild onion (Allium canadense L.) were used to study the effects of four surfactants on cell permeability as measured by loss of intracellular material and on photosynthetic 14CO2 fixation. Surfactant-induced alteration in cell permeability could not be related to surfactant-ionogenic class but could be related to reported phytotoxicity. Surfactants that altered cell permeability also inhibited photosynthetic 14CO2 fixation, indicating that surfactant effects were not restricted to the outer-cell membrane. Isolated cells from the monocotyledonous wild onion were more susceptible to surfactant-induced alterations in cell permeability than were cells from the dicotyledonous soybean. Efflux of intracellular material was modified by Ca++ and Mg++ to a greater extent for soybean than for wild onion. Integrity of the isolated cells, before and after surfactant treatment, was verified by microscopic observation.

Type
Research Article
Information
Weed Science , Volume 22 , Issue 3 , May 1974 , pp. 233 - 237
Copyright
Copyright © 1974 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Beauchamp, E.G. 1972. Manganese and zinc absorption with octylphenoxyethanol surfactants by soybean leaf tissues. Can. J. Bot. 51:613–620.CrossRefGoogle Scholar
2. Behrens, R.W. 1964. The physical and chemical properties of surfactants and their effects on formulated herbicides. Weeds 12:255–258.CrossRefGoogle Scholar
3. Bolware, M.A. and Camper, N.D. 1973. Sorption of some 14C-herbicides by isolated plant cells and protoplasts. Weed Sci. 21:145–149.CrossRefGoogle Scholar
4. Haapala, E. 1970. The effect of a non-ionic detergent on some plant cells. Physiol. Plant. 23:187–201.CrossRefGoogle Scholar
5. Jackson, W.T. 1962. Use of carbowaxes (polyethylene glycols) as osmotic agents. Plant Physiol. 37:513–519.CrossRefGoogle ScholarPubMed
6. Jansen, L.L., Gentner, W.A., and Shaw, W.C. 1961. Effects of surfactants on the herbicidal activity of several herbicides in aqueous spray systems. Weeds 9:381–405.CrossRefGoogle Scholar
7. Jansen, L.L. 1964. Surfactant enhancement of herbicide entry. Weeds 12:251–254.CrossRefGoogle Scholar
8. Jansen, L.L. 1973. Enhancement of herbicides by silicone surfactants. Weed Sci. 21:130–135.CrossRefGoogle Scholar
9. Nethery, A.A. 1967. Inhibition of mitosis by surfactants. Cytologia 32:321–327.CrossRefGoogle ScholarPubMed
10. Neumann, J. and Jagendorf, A. 1965. Uncoupling photophosphorylation by detergents. Biochim. Biophys. Acta 109:382–389.CrossRefGoogle ScholarPubMed
11. Rehfeld, D.W. and Jensen, R.G. 1973. Metabolism of separated leaf cells. III. Effect of Ca++ and ammonium on product distribution during photosynthesis with cotton cells. Plant Physiol. 52:17–22.CrossRefGoogle ScholarPubMed
12. Sikka, C., Saxena, J., and Zweig, G. 1973. Alteration in cell permeability as a mechanism of action of certain quinone pesticides. Plant Physiol. 51:363–367.CrossRefGoogle ScholarPubMed
13. Smith, L.W. and Foy, C.L. 1966. Penetration and distribution studies in bean, cotton and barley from foliar and root applications of Tween 20-14C, fatty acid and oxyethylene labeled. J. Agr. Food Chem. 14:117–122.CrossRefGoogle Scholar
14. Sutton, D.L. and Foy, C.L. 1971. Effect of diquat and several surfactants on membrane permeability in red beet root tissue. Bot Gaz. 132:299–304.CrossRefGoogle Scholar