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Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis)

Published online by Cambridge University Press:  20 January 2017

Clifford H. Koger  and
Krishna N. Reddy
Krishna N. Reddy
Affiliation:
U.S. Department of Agriculture–Agricultural Research Service, Southern Weed Science Research Unit, P.O. Box 350, Stoneville, MS 38776
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Abstract

Greenhouse and laboratory experiments were conducted to investigate mechanisms of glyphosate resistance in horseweed populations from Mississippi, Arkansas, Delaware, and Tennessee. A nondestructive leaf-dip bioassay was developed to confirm resistance and susceptibility in individual test plants. A single leaf was excised from each plant, and the petiole and bottom one-fourth of leaf was dipped in a 600 mg ae L−1 glyphosate solution for 2 d followed by visually estimating the injury on a scale of 0 to 10. Plants were classified as resistant (R) if the score was 2 to 3 and susceptible (S) if the score was 5 to 6. 14C-glyphosate solution was applied on the adaxial surface of a fully expanded leaf of the second whorl of four-whorl rosette plants. Plants were harvested 48 h after treatment and radioactivity was determined in treated leaf, other leaves, crown, and roots. Absorption of 14C-glyphosate was similar (47 to 54%) between R and S plants from within and among the four states, suggesting absorption is not involved in glyphosate resistance. The amount of radioactivity translocated from the treated leaf was reduced in R plants compared with S plants. The reduction in translocation of 14C-glyphosate ranged from 28% in Mississippi-R biotype to 48% in Delaware-R biotype compared with their respective S biotypes. Epicuticular wax mass ranged from 6 to 80 μg cm−2 among horseweed biotypes, with no differences between R and S biotypes within each state. Treating two leaves with glyphosate solution at the field use rate (0.84 kg ae ha−1) killed S plants but not R plants (38 to 58% control) regardless of state origin. These results suggest that a simple bioassay can be used to screen biotypes for suspected resistance and that reduced translocation of glyphosate plays a major role in glyphosate resistance in R biotypes of horseweed.

Keywords

14C-glyphosateglyphosatehorseweed, Conyza canadensis (L.) CronqBioassaycuticleepicuticular waxglyphosateglyphosate resistanceherbicide efficacyweed resistance
Type
Weed Biology and Ecology
Information
Weed Science , Volume 53 , Issue 1 , February 2005 , pp. 84 - 89
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Baerson, S. R., Rodriguez, D. J., Biest, N. A., Tran, M., You, J., Kreuger, R. W., Dill, G. M., Pratley, J. E., and Gruys, K. J. 2002. Investigating the mechanism of glyphosate resistance in rigid ryegrass (Lolium rigidum). Weed Sci 50:721–730.Google Scholar
Camacho, R. F. and Moshier, L. J. 1991. Absorption, translocation, and activity of CGA-136872, DPX-V9360, and glyphosate in rhizome johnsongrass (Sorghum halepense). Weed Sci 39:354–357.Google Scholar
Chachalis, D., Reddy, K. N., Elmore, C. D., and Steele, M. L. 2001. Herbicide efficacy, leaf structure, and spray droplet contact angle among Ipomoea species and smallflower morningglory. Weed Sci 49:628–634.Google Scholar
Duke, S. O. 1988. Glyphosate. Pages 1–70 in Kearney, P. C. and Kaufman, D. D. eds. Herbicides: Chemistry, Degradation, and Mode of Action. New York: Marcel Dekker.Google Scholar
Feng, P. C., Tran, M., Chiu, T., Sammons, R. D., Heck, G. R., and CaJacob, C. A. 2004. Investigation into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation, and metabolism. Weed Sci 52:498–505.Google Scholar
Feng, P. C. C., Pratley, J. E., and Bohn, J. A. 1999. Resistance to glyphosate in Lolium rigidum. II. Uptake, translocation, and metabolism. Weed Sci 47:412–415.Google Scholar
Ferreira, J. F. S. and Reddy, K. N. 2000. Absorption and translocation of glyphosate in Erythroxylum coca and E. novogranatense . Weed Sci 48:193–199.Google Scholar
Gillespie, G. R. 1994. Basis for the differential response of quackgrass (Elytrigia repens) biotypes to rimsulfuron. Weed Sci 42:8–12.Google Scholar
Heap, I. 2004. The International Survey of Herbicide Resistant Weeds. www.weedscience.com.Google Scholar
Koger, C. H., Poston, D. H., Hayes, R. M., and Montgomery, R. F. 2004. Glyphosate-resistant horseweed (Conyza canadensis) in Mississippi. Weed Technol 18:820–825.Google Scholar
Lee, L. J. and Ngim, J. 2000. A first report of glyphosate-resistant goosegrass (Eleusine indica (L.) Gaertn) in Malaysia. Pest Manag. Sci 56:336–339.Google Scholar
McWhorter, C. G. 1993. Epicuticular wax on johnsongrass (Sorghum halepense) leaves. Weed Sci 41:475–482.Google Scholar
Mueller, T. C., Massey, J. H., Hayes, R. M., Main, C. L., and Stewart, C. N. Jr. 2003. Shikimate accumulation in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza Canadensis L. Cronq). J. Agric. Food Chem 51:680–684.Google Scholar
Powles, S. B., Lorraine-Colwill, D. F., Dellow, J. J., and Preston, C. 1998. Evolved resistance to glyphosate in rigid ryegrsss (Lolium rigidum) in Australia. Weed Sci 46:604–607.Google Scholar
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J., and Krueger, R. 1999. Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci 47:405–411.Google Scholar
Reddy, K. N. 2000. Factors affecting toxicity, absorption, and translocation of glyphosate in redvine (Brunnichia ovata). Weed Technol 14:457–462.Google Scholar
Simarmata, M. and Penner, D. 2004. Role of EPSP synthase in glyphosate resistance in rigid ryegrass (Lolium rigidum Gaud). Weed Sci. Soc. Am. Abstr 44:34.Google Scholar
VanGessel, M. J. 2001. Glyphosate-resistant horseweed from Delaware. Weed Sci 49:703–705.Google Scholar