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A Rapid Microtitre Plate Assay for Determining Sensitivity to Photosystem II Herbicides

Published online by Cambridge University Press:  12 June 2017

Michael P. Anderson ,
Curtis N. Bensch  and
Jimmy F. Stritzke
Michael P. Anderson
Affiliation:
Dep. Agron. Oklahoma State Univ., Stillwater, OK 74078
Curtis N. Bensch
Affiliation:
Dep. Agron. Oklahoma State Univ., Stillwater, OK 74078
Jimmy F. Stritzke
Affiliation:
Dep. Agron. Oklahoma State Univ., Stillwater, OK 74078
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Abstract

This paper reports on a microtitre plate version of the 2,6-dichlorophenol-indophenol (DCPIP) reduction assay. DCPIP reduction rates of alfalfa thylakoid membranes were determined by measuring absorbance at 600 nm before and after a 1 min illumination period. The membranes were near light-saturated at intensities above 600 μE m–2 s–1. Saturation of thylakoid membranes with DCPIP occurred above 120 μM. DCPIP reduction rates increased linearly with chlorophyll concentrations from 0.25 to 2 μg. The rate of DCPIP reduction was linear throughout a 2 min illumination period (R2 = 0.99). The assay was sensitive enough to terbacil to differentiate between a 20% change in the I50 concentration. DCPIP reduction rates were sensitive to concentrations of terbacil as low as 100 nM. It only takes 13 minutes to load and read 96 samples using the microtitre plate assay compared to 5.5 h using the conventional procedure.

Keywords

Terbacil, 5-chloro-3-(1,1-dimethylethyl)-6-methyl-2,4-(1H,3H)-pyrimidinedionealfalfa, Medicago sativa L. # MEDSATerbacilatrazineMedicago sativa L.
Type
Physiology, Chemistry, and Biochemostry
Information
Weed Science , Volume 42 , Issue 4 , December 1994 , pp. 517 - 522
Copyright
Copyright © 1994 by the Weed Science Society of America 

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References

Literature Cited

1. Ahrens, W. H., Arntzen, C. J., and Stoller, E. W. 1981. Chlorophyll fluorescence assay for the determination of triazine resistance. Weed Sci. 29:316–322.Google Scholar
2. Amon, D. I. 1949. Copper enzymes in isolated chloroplasts: Polyphenoloxidase in (Beta vulgaris). Plant Physiol. 24:1–5.Google Scholar
3. Conard, S. G., and Radosevich, S. R. 1979. Ecological fitness of Senicio vulgaris and Amarathus retroflexus biotypes susceptible or resistant to atrazine. J. Appl. Ecol. 16:171–177.CrossRefGoogle Scholar
4. Darmency, H. and Pernes, J. 1985. Use of wild Sefaria viridis to improve triazine resistance in cultivated S. italica by hybridization. Weed Res. 25:175–179.Google Scholar
5. Darr, S., Souza-Machado, V., and Arntzen, C. J. 1981. Uniparental inheritance of a chloroplast photosystem II polypeptide controlling herbicide binding. Biochim. et. Biophys. Acta. 634:219–228.Google Scholar
6. Faulkner, J. S. 1982. Breeding herbicide-tolerant crop cultivars by conventional methods. Pages 235–256 in Lebaron, H. M. and Gressel, J., eds. Herbicide Resistance in Plants. John Wiley & Sons, New York.Google Scholar
7. Gressel, J. and Ben-Sinai, G. 1985. Low intraspecific competitive fitness in a triazine-resistant, nearly nuclear-isogenic line of Brassica napus . Plant Sci. 38:29–32.Google Scholar
8. Gronwald, J. W., Andersen, R. N., and Yee, C. 1989. Atrazine resistance in velvetleaf (Abutilon theophrasti) due to enhanced atrazine detoxification. Pest. Biochem. Physiol. 34:149–163.Google Scholar
9. Harris, M. and Camlin, M. S. 1988. Chlorophyll fluorescence as a rapid test for reaction to urea herbicides in winter wheat. Agric. Sci. Camb. 110:627–632.Google Scholar
10. Holt, J. S. and Lebaron, H. M. 1990. Significance and distribution of herbicide resistance. Weed Technol. 4:141–149.Google Scholar
11. Kube, J. G., Vogel, K. P., and Moser, L. E. 1989. Genetic variability for seedling atrazine tolerance in indiangrass. Crop Sci. 29:18–23.Google Scholar
12. Mets, L. and Thiel, A. 1989. Biochemistry and genetic control of the photosystem II herbicide target site. Pages 2–3 in Boger, Peter and Sandmann, Gerhard, eds. Target Sites of Herbicide Action. CRC Press, Boca Raton, FL.Google Scholar
13. Marc, P., Leroux, G. D., and Carpentier, R. 1990. Atrazine action on the donor side of photosystem II in triazine-resistant and -susceptible weed biotypes. Pestic. Biochem. Physiol. 37:83–89.Google Scholar
14. Purcell, M., Leroux, G. D., and Carpentier, R. 1990. Atrazine action on the donor side of photosystem II in triazine and -susceptible weed biotypes. Pest. Biochem. and Physiol. 37:83–89.Google Scholar
15. Radosevich, S. R., Steinback, K. E., and Arntzen, C. J. 1979. Effect of photosystem II inhibitors on thylakoid membranes of two common groundsel (Senecio vulgaris) biotypes. Weed Sci. 27:216–218.Google Scholar
16. Ratliff, R. L., Carver, B. F., and Peeper, T. F. 1991. Expression of metribuzin sensitivity in winter wheat (Triticum aestivum) populations. Weed Sci. 39:130–133.Google Scholar
17. Schneider, P. and Hammock, B. D. 1992. Influence of the ELISA format and the hapten-enzyme conjugate on the sensitivity of an immunoassay for s-triazine herbicides using monoclonal antibodies. J. Agric. Food Chem. 40:525–530.Google Scholar
18. Shaw, D. R., Peeper, T. F., and Nofzinger, D. L. 1985. Comparison of chlorophyll fluorescence and fresh weight as herbicide bioassay techniques. Weed Sci. 33:29–33.Google Scholar
19. Souza-Machado, V. 1982. Inheritance and breeding potential of triazine tolerance and resistance in plants. Pages 257–273 in Lebaron, H. M. and Gressel, J., eds. Herbicide Resistance in Plants. John Wiley & Sons, New York.Google Scholar
20. Souza-Machado, V., Arntzen, C. J., Bandeen, J. D., and Stephenson, G. R. 1978. Comparative triazine effects upon system II photochemistry in chloroplasts of two common lambsquarters (Chenopodium album) biotypes. Weed Sci. 26:318–322.Google Scholar
21. Stephenson, G. R., Dykstra, M. D., McLaren, R. D., and Hamill, A. S. 1990. Agronomic practices influencing triazine-resistant weed distribution in Ontario. Weed Technol. 4:199–207.Google Scholar
22. Truelove, B. and Hensley, J. R. 1981. Methods of testing for herbicide resistance. In Lebaron, H. M. and Gressel, J., eds. Herbicide Resistance in Plants. John Wiley & Sons, New York.Google Scholar
23. Warwick, S. I. and Black, L. 1981. The relative competitiveness of atrazine susceptible and resistant populations of Chenopodium album and C. strictum . Can. J. Bot. 59:689–693.CrossRefGoogle Scholar