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Molecular Basis of Resistance to ALS-Inhibitor Herbicides in Greater Beggarticks

Published online by Cambridge University Press:  20 January 2017

Fabiane P. Lamego ,
Dirk Charlson ,
Carla A. Delatorre ,
Nilda R. Burgos  and
Ribas A. Vidal
Fabiane P. Lamego*
Affiliation:
CNPq/Brazil and PPG Fitotecnia, School of Agronomy, Federal University of Rio Grande do Sul (UFRGS), Av Bento Gonçalves 7712, Cx Postal 15100, 90001–970, Porto Alegre, RS, Brazil
Dirk Charlson
Affiliation:
Crop Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701
Carla A. Delatorre
Affiliation:
CNPq/Brazil and PPG Fitotecnia, School of Agronomy, Federal University of Rio Grande do Sul (UFRGS), Av Bento Gonçalves 7712, Cx Postal 15100, 90001–970, Porto Alegre, RS, Brazil
Nilda R. Burgos
Affiliation:
Crop Soil and Environmental Sciences, University of Arkansas, Fayetteville, AR, 72701
Ribas A. Vidal
Affiliation:
CNPq/Brazil and PPG Fitotecnia, School of Agronomy, Federal University of Rio Grande do Sul (UFRGS), Av Bento Gonçalves 7712, Cx Postal 15100, 90001–970, Porto Alegre, RS, Brazil
*
Corresponding author's E-mail: [email protected]
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Abstract

Soybean is a major crop cultivated in Brazil, and acetolactate synthase (ALS)-inhibiting herbicides are widely used to control weeds in this crop. The continuous use of these ALS-inhibiting herbicides has led to the evolution of herbicide-resistant weeds worldwide. Greater beggarticks is a polyploid species and one of the most troublesome weeds in soybean production since the discovery of ALS-resistant biotypes in 1996. To confirm and characterize the resistance of greater beggarticks to ALS inhibitors, whole-plant bioassays and enzyme experiments were conducted. To investigate the molecular basis of resistance in greater beggarticks the ALS gene was sequenced and compared between susceptible and resistant biotypes. Our results confirmed that greater beggarticks is resistant to ALS inhibitors and also indicated it possesses at least three isoforms of the ALS gene. Analysis of the nucleotide and deduced amino acid sequences among the isoforms and between the biotypes indicated that a single point mutation, G–T, in one ALS isoform from the resistant biotype resulted in an amino acid substitution, Trp574Leu. Two additional substitutions were observed, Phe116Leu and Phe149Ser, in a second isoform of the resistant biotype, which were not yet reported in any other herbicide-resistant ALS gene; thus, their role in conferring herbicide resistance is not yet ascertained. This is the first report of ALS mutations in an important, herbicide-resistant weed species from Brazil.

Keywords

Chlorimuroncloransulamimazethapyrpyrithiobacgreater beggarticks, Bidens subalternans DCsoybean, Glycine max (L.) Merr.Acetolactate synthaseALS geneBidensherbicide resistancepolyploidy
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 57 , Issue 5 , October 2009 , pp. 474 - 481
Copyright
Copyright © Weed Science Society of America 

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