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A molecular approach to understanding root bud dormancy in leafy spurge

Published online by Cambridge University Press:  20 January 2017

David P. Horvath  and
James V. Anderson
James V. Anderson
Affiliation:
U.S. Department of Agriculture, Agricultural Research Service, Plant Science Research, 1605 Albrecht Blvd., P.O. Box 5674, State University Station, Fargo, ND 58105
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Abstract

Leafy spurge is a tenacious perennial weed of the Northern Plains. This plant maintains a perennial growth cycle by controlled production and growth of numerous underground adventitious buds. We are using molecular tools to identify signaling pathways that control underground adventitious bud growth and development in leafy spurge. Toward this end, we have used three techniques to identify genes that are differentially expressed concomitantly with the breaking of quiescence in underground buds of leafy spurge. These techniques include differential display of cDNAs, random cloning and sequencing of genes expressed in growing buds, and microarray technology. To date, we have identified more than 16 genes that are differentially expressed in underground buds of leafy spurge during dormancy break and growth initiation. A detailed expression analysis of these genes will allow them to be grouped by their responses to various signals known to play a role in control of underground bud growth. This information will be used to identify key cis-acting elements involved in the regulation of these genes. How such information on signal transduction processes may be used for developing new weed control strategies by the identification of novel target pathways and development of DNA-based herbicides is presented.

Keywords

Leafy spurge, Euphorbia esula L. EPHESReproductionvegetative propagules
Type
Research Article
Information
Weed Science , Volume 50 , Issue 2 , April 2002 , pp. 227 - 231
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Ascenzi, R., Boyles, D. C., Zayed, A. M., et al. 2001 Identification of herbicide targets using a high-throughput functional genomics approach. Proceedings of the 12th International Conference on Arabidopsis Research. Madison, WI.Google Scholar
Beveridge, C. A., Symons, G. M., and Turnbull, C. G. N. 2000. Auxin inhibition of decapitation-induced branching is dependent on graft-transmissible signals regulated by genes Rms1 and Rms2 . Plant Physiol. 123:689–698.Google Scholar
Chang, S., Puryear, J., and Cairney, J. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Reporter 11:113–116.CrossRefGoogle Scholar
Chao, W. S., Anderson, J. V., and Horvath, D. P. 2000. Sugar plays a role in inhibition of underground adventitious bud growth in leafy spurge (Euphorbia esula L.). ASPP Plant Biol. 46.Google Scholar
Cline, M. G. 1991. Apical dominance. Bot. Rev. 57:318–358.Google Scholar
Gaudin, V., Lunness, P. A., Fobert, P. R., Towers, M., Riou-Khamlichi, C., Murray, J. A. H., Coen, E., and Doonan, J. H. 2000. The expression of D-cyclin genes defines distinct developmental zones in snapdragon apical meristems and is locally regulated by the cycloidea gene. Plant Physiol. 122:1137–1148.Google Scholar
Gendreau, E., Hofte, H., Orbovic, V., and Traas, J. 1999. Gibberellin and ethylene control endoreduplication levels in the Arabidopsis thaliana hypocotyl. Planta 209:513–516.Google Scholar
Girke, T., Todd, J., Ruuska, S., White, J., Benning, C., and Ohlrogge, J. 2000. Microarray analysis of developing Arabidopsis seeds. Plant Physiol. 124:1570–1581.Google Scholar
Horvath, D. P. 1998. The role of specific plant organs and polar auxin transport in correlative inhibition of leafy spurge (Euphorbia esula) root buds. Can. J. Bot. 76:1227–1231.Google Scholar
Horvath, D. P. 1999. Role of mature leaves in inhibition of root bud growth in Euphorbia esula . Weed Sci. 47:544–550.Google Scholar
Horvath, D. P. and Olson, P. A. 1998. Cloning and characterization of cold-regulated glycine-rich RNA-binding protein genes from leafy spurge (Euphorbia esula L.) and comparison to heterologous genomic clones. Plant Mol. Biol. 38:531–538.Google Scholar
Huijser, C., Kortstee, A., Pego, J., Weisbeek, P., Wisman, E., and Smeekens, S. 2000. The Arabidopsis SUCROSE UNCOUPLED-6 gene is identical to ABSCISIC ACID INSENSITIVE-4: involvement of absisic acid in sugar responses. Plant J. 23:577–585.Google Scholar
Laby, R. J., Kincaid, M. S., Kim, D., and Gibson, S. I. 2000. The Arabidopsis sugar-insensitive mutants sis4 and sis5 are defective in absiscic acid synthesis and response. Plant J. 23:587–596.Google Scholar
Leyser, H. M. O., Timpte, L. C. A., Lammer, D., Turner, J., and Estelle, M. 1993. Arabidopsis auxin-resistance gene AXR1 encodes a protein related to ubiquitin-activating enzyme E1. Nature 364:161–164.Google Scholar
Nissen, S. J. and Foley, M. E. 1987. Correlative inhibition in root buds of leafy spurge. Weed Sci. 35:155–159.CrossRefGoogle Scholar
Perata, P., Matsukura, C., Vernieri, P., and Yamaguchi, J. 1997. Sugar repression of a gibberellin-dependant signaling pathway in barley embryos. Plant Cell 9:2197–2208.Google Scholar
Sambrook, J., Fritsch, E. F., and Maniatis, T. 1989. Molecular Cloning. 2nd ed. New York: Cold Spring Harbor Laboratory Press. pp. New York.39-7.52.Google Scholar
Sauter, M., Mekhedov, S. L., and Kende, H. 1995. Gibberellin promotes histone H1 kinase activity and the expression of cdc2 and cyclin genes during the induction of rapid growth in deepwater rice internodes. Plant J. 7:623–632.Google Scholar
Soni, R., Carmichael, J. P., Shah, Z. H., and Murray, J. A. 1995. A family of cyclin D homologues from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif. Plant Cell 7:85–103.Google Scholar
Stafstrom, J. P., Ripley, B. D., Devitt, M. L., and Drake, B. 1998. Dormancy-associated gene expression in pea axillary buds. Cloning and expression of PsDRM1 and PsDRM2. Planta 205:547–552.Google Scholar
Xu, X., van Lammeren, A. A. M., Vermeer, E., and Vreugdenhil, D. 1998. The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol. 117:575–584.CrossRefGoogle ScholarPubMed