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Metabolic changes, agronomic performance, and quality of seeds in soybean with the pat gene after application of glufosinate

Published online by Cambridge University Press:  02 October 2020

Alfredo Junior P. Albrecht ,
Ivana Paula F. S. de Brito ,
Leandro P. Albrecht ,
André Felipe M. Silva Open the ORCID record for André Felipe M. Silva [Opens in a new window] ,
Ana Karollyna A. de Matos ,
Caio Antonio Carbonari  and
Edivaldo D. Velini
Alfredo Junior P. Albrecht
Affiliation:
Professor, Federal University of Paraná, Palotina, Paraná, Brazil
Ivana Paula F. S. de Brito
Affiliation:
Graduate Student, São Paulo State University, School of Agriculture, Botucatu, São Paulo, Brazil
Leandro P. Albrecht
Affiliation:
Professor, Federal University of Paraná, Palotina, Paraná, Brazil
André Felipe M. Silva*
Affiliation:
Research Scientist, Crop Science, Palotina, Paraná, Brazil
Ana Karollyna A. de Matos
Affiliation:
Graduate Student, São Paulo State University, School of Agriculture, Botucatu, São Paulo, Brazil
Caio Antonio Carbonari
Affiliation:
Professor, São Paulo State University, School of Agriculture, Botucatu, São Paulo, Brazil
Edivaldo D. Velini
Affiliation:
Professor, São Paulo State University, School of Agriculture, Botucatu, São Paulo, Brazil
*
Author for correspondence: André Felipe Moreira Silva, Crop Science, Palotina, Rodovia PR 364, no. 3741, 85950-000, Paraná, Brazil. (Email: [email protected])
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Abstract

The transgenic Liberty Link® (LL) soybean is tolerant to glufosinate, conferred by the enzyme phosphinothricin acetyltransferase (PAT), which is encoded by the pat gene from Streptomyces viridochromogenes. Because symptoms of injury can be observed in soybean [Glycine max (L.) Merr.] plants in some situations, this study evaluated the effects of rates of glufosinate on agronomic performance; quality of LL soybean seeds; and the ammonia, glufosinate, and N-acetyl-l-glufosinate concentration (NAG) in soybeans with and without the pat gene after application of increasing glufosinate rates. Field and greenhouse experiments were conducted; the first evaluated the selectivity of glufosinate in LL soybeans, and the second evaluated the metabolic changes in soybeans with (LL) and without (RR2) the pat gene, after application of glufosinate. For fieldwork, application of glufosinate at rates up to four times the maximum recommended caused initial injury symptoms (up to 38.5%) in LL soybean plants. However, no negative effect was found on seed quality and agronomic performance of LL plants, including yield. This shows the selectivity of glufosinate promoted by pat gene insertion for application in POST (V4), in LL soybean. For the greenhouse experiment, it was concluded that the LL soybean plants presented high glufosinate metabolism, lower ammonia concentration, and no reduction in dry matter, in comparison with RR2 soybean, after application of high rates of glufosinate.

Keywords

Crop injuryglufosinate-tolerant soybeanglutamine synthetaseN-acetyl-l-glufosinateselectivity
Type
Research Article
Information
Weed Science , Volume 68 , Issue 6 , November 2020 , pp. 594 - 604
Copyright
© The Author(s), 2020. Published by Cambridge University Press on behalf of the Weed Science Society of America

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Footnotes

Associate Editor: Franck E. Dayan, Colorado State University

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