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The in Vitro Culture of Excised Ovules from Velvetleaf (Abutilon theophrasti)

Published online by Cambridge University Press:  12 June 2017

Laura K. Thompson ,
Gerald R. Leather  and
Maynard G. Hale
Laura K. Thompson
Affiliation:
Dep. Plant Pathol. and Physiol., Virginia Polytech. Inst. and State Univ., Blacksburg, VA 24061
Gerald R. Leather
Affiliation:
Weed Sci. Res., Agric. Res. Serv., U.S. Dep. Agric., Bldg. 1301, Ft. Detrick, Frederick, MD 21701
Maynard G. Hale
Affiliation:
Dep. Plant Pathol. and Physiol., Virginia Polytech. Inst. and State Univ., Blacksburg, VA 24061
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Abstract

A culture medium and environmental conditions were selected for ovule culture of velvetleaf (Abutilon theophrasti Medic. ♯4 ABUTH), which allowed development parallel to that occurring in vivo. Of the six nutrient media evaluated, the most suitable one contained an organic nitrogen source and was of high osmotic potential. A liquid medium with a pH of 5 to 7, and culture for 14 days at 25 C under green light (1.3 μmole·m-2·-1) encouraged good embryo and ovule development. No difference was recorded between polyethylene glycol 20000 and mannitol in the medium used for dehydration and subsequent imbibition of cultured mature ovules. To investigate the effects of maternal tissues, surgical alterations were performed on the testa and funiculus of immature velvetleaf ovules. The intact testa appears to be a barrier to nutrient absorption and reduces the fresh and dry weights and embryo length. Ovule and embryo development was significantly better if either the testa was nicked or the funiculus removed.

Keywords

Ovule cultureembryo developmentseed developmentseed dormancyABUTH
Type
Weed Biology and Ecology
Information
Weed Science , Volume 32 , Issue 6 , November 1984 , pp. 792 - 797
Copyright
Copyright © 1984 by the Weed Science Society of America 

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