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Growth Response of Wheat (Triticum aestivum) Callus to Imazapyr and in Vitro Selection for Resistance

Published online by Cambridge University Press:  12 June 2017

David C. Heering ,
Arron C. Guenzi ,
Thomas F. Peeper  and
P. L. Claypool
David C. Heering
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078-0507
Arron C. Guenzi
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078-0507
Thomas F. Peeper
Affiliation:
Dep. Agron., Oklahoma State Univ., Stillwater, OK 74078-0507
P. L. Claypool
Affiliation:
Dep. Statistics, Oklahoma State Univ., Stillwater, OK 74078-0507
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Abstract

Intact wheat plants and wheat calli responded similarly to varying concentrations of imazapyr. Fifty percent growth inhibition of wheat callus occurred with 0.05 μM imazapyr after 70 d. As imazapyr concentration increased from 0 to 10 μM, the free isoleucine, leucine, and valine decreased from 160 to 35, 260 to 49, and 310 to 59 pmol mg−1, respectively. Resistant calli, which had relative growth rates exceeding a calculated upper prediction interval, were obtained by in vitro selection at 2 and 5 μM imazapyr. Resistant calli growing on 2 μM imazapyr had free isoleucine, leucine, and valine concentrations intermediate to the control and susceptible callus.

Keywords

Imazapyr, (±)-2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-pyridinecarboxylic acid2,4-D (2,4-dichlorophenoxy)acetic acidwheat, Triticum aestivum L. em. Thell.‘Bobwhite′Mutationsherbicide resistanceamino acidsimidazolinones
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 40 , Issue 2 , June 1992 , pp. 174 - 179
Copyright
Copyright © 1992 by the Weed Science Society of America 

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References

Literature Cited

1. Anderson, P. C. and Georgeson, M. 1989. Herbicide-tolerant mutants of corn. Genome 31:994–999.CrossRefGoogle Scholar
2. Anderson, P. C. and Hibberd, K. A. 1985. Evidence for the interaction of an imidazolinone herbicide with leucine, valine, and isoleucine metabolism. Weed Sci. 33:479–483.CrossRefGoogle Scholar
3. Ayala, F. J. and Kiger, J. A. 1980. Pages 544–551 in Modern Genetics. Benjamin/Cummings Publ. Co., Inc., Menlo Park, CA.Google Scholar
4. Bieleski, R. L. and Turner, N. A. 1966. Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Anal. Biochem. 17:278–293.CrossRefGoogle Scholar
5. Chaleff, R. S. and Mauvais, C. 1984. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science 224:1443–1445.CrossRefGoogle ScholarPubMed
6. Chaleff, R. S. 1983. Isolation of agronomically useful mutants from plant cell culture. Science 219:676–682.CrossRefGoogle Scholar
7. Chaleff, R. S. and Parsons, N. 1978. Direct selection in vitro for herbicide resistant mutants of Nicotiana tabacum . Proc. Nat. Acad. Sci (U.S.A.) 75:5704–5707.CrossRefGoogle ScholarPubMed
8. Cohen, S. A., Tarvin, T. L., Bidlingmeyer, B. A., and Tarr, G. E. 1984. Analysis of amino acids using pre-column derivatization with phenylisothiocyanate. Am. Lab. 16(12):48–49.Google Scholar
9. Heering, D. C. and Peeper, T. F. 1991. Field bindweed (Convolvulus arvensis) control in winter wheat (Triticum aestivum) with herbicides. Weed Technol. 5:411–415.CrossRefGoogle Scholar
10. Heinrikson, R. L. and Meredith, S. C. 1984. Amino acid analysis by reverse phase high performance liquid chromatography: Pre-column derivatization with phenylisothyocyanate. Anal. Biochem. 136:65–74.CrossRefGoogle Scholar
11. Hoagland, D. R. and Arnon, D. I. 1950. The water-culture method for growing plants without soil. Calif. Agric. Exp. Sta. Circ. 347.Google Scholar
12. Hughes, K. 1983. Selection for herbicide resistance. Pages 442–522 in Evans, D. A., Sharp, W. R., Ammirato, P. V., Yamada, Y., eds. Handbook of Plant Cell Culture: Techniques for Propagation and Breeding. Vol 1. The MacMillan Co., New York.Google Scholar
13. Klein, R. and Chaleff, R. S. 1983. Genetic characterization of hydroxyurea resistant mutants obtained from cell cultures of Nicotiana tabacum . Mol. Gen. Genet. 192:218–224.Google Scholar
14. Larkin, P. J., Banks, P. M., Bhati, R., Brettell, R.I.S., Davies, P. A., Ryan, S. A., Scowcroft, W. R., Spindler, L. H., and Tanner, G. J. 1989. From somatic variation to variant plants: Mechanisms and applications. Genome 31:705–711.CrossRefGoogle Scholar
15. Muhitch, M J., Shaner, D. L., and Stidham, M. A. 1987. Imidazolinones and acetohydroxyacid synthase from higher plants. Plant Physiol. 83:451–456.CrossRefGoogle ScholarPubMed
16. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant 15:475–497.CrossRefGoogle Scholar
17. Parker, W. B., Marshall, L. C., Gurton, J. D., Somers, D. A., Wyse, D. L., Gronwald, J. W., and Gengenbach, B. G. 1990. Dominant mutations causing alterations in acetyl-coenzyme A carboxylase confer tolerance to cyclohexanedione and aryloxyphenoxypropionate herbicides in maize. Proc. Nat Acad. Sci. (U.S.A.) 87:7175–7179.CrossRefGoogle ScholarPubMed
18. Sears, R. G. and Deckard, E. L. 1982. Tissue culture variability in wheat callus induction and plant regeneration. Crop Sci. 22:546–550.CrossRefGoogle Scholar
19. Thomas, B. and Pratt, D. 1982. Isolation of paraquat tolerant mutants in tomato cell cultures. Theor. Appl. Genet 63:169–176.CrossRefGoogle ScholarPubMed
20. Shaner, D. L. 1989. Factors affecting soil and foliar bioavailability of imidazoliones. American Cyanamid, Princeton, NJ.Google Scholar
21. Shaner, D. L. and Reider, M. L. 1986. Physiological responses of corn (Zea mays) to AC 243,997 in combination with valine, leucine, and isoleucine. Pestic. Biochem. Physiol. 25:248–257.CrossRefGoogle Scholar
22. Singer, S. R. and McDaniel, C. N. 1986. Analyzing growth in cell cultures. Effects of initial cell mass on growth. Can. J. Bot. 64:328–341.Google Scholar
23. Steel, R.G.D. and Torrie, J. H. 1980. Principles and Procedures of Statistics: A Biomedical Approach. 2nd ed. McGraw-Hill Book Co., New York.Google Scholar
24. Swanson, E. B., Coumans, M. P., Brown, G. L., Patel, J. D., and Beversdorf, W. D. 1988. The characterization of herbicide tolerant plants in Brassica napus L. after in vitro selection of microspores and protoplasts. Plant Cell Rep. 7:83–87.CrossRefGoogle ScholarPubMed
25. Swanson, E. B., Herrgesell, M. J., Arnoldo, M., Sippell, D. W., and Wong, R.S.C. 1989. Microspore mutagenesis and selection: Canola plants with field tolerance to the imidazolinones. Theor. Appl. Genet. 78:525–530.CrossRefGoogle Scholar
26. Zadoks, J. C., Chang, T. T., and Konzak, C. F. 1974. A decimal code for the growth stages of cereals. Weed Res. 14:415–421.CrossRefGoogle Scholar