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Genetic Relationships between Tropical Sprangletop (Leptochloa virgata) Populations from Mexico: Understanding Glyphosate Resistance Spread

Published online by Cambridge University Press:  20 January 2017

Ricardo Alcántara-de la Cruz ,
Yolanda Romano ,
María Dolores Osuna-Ruíz ,
José Alfredo Domínguez-Valenzuela ,
Julio Menéndez  and
Rafael De Prado
Ricardo Alcántara-de la Cruz*
Affiliation:
Agricultural Chemistry and Edaphology, University of Cordoba, 14071 Cordoba, Spain
Yolanda Romano
Affiliation:
Agrarian Research Center “Finca La Orden Valdesequera”, 60187, Badajoz, Spain
María Dolores Osuna-Ruíz
Affiliation:
Agrarian Research Center “Finca La Orden Valdesequera”, 60187, Badajoz, Spain
José Alfredo Domínguez-Valenzuela
Affiliation:
Agricultural Parasitology, Chapingo Autonomous University, 56230 Chapingo, Mexico
Julio Menéndez
Affiliation:
Agroforestry Sciences, University of Huelva, 21819, Huelva, Spain
Rafael De Prado
Affiliation:
Agricultural Chemistry and Edaphology, University of Cordoba, 14071 Cordoba, Spain
*
Corresponding author's E-mail: [email protected]
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Abstract

The susceptibility to glyphosate and genetic diversity based on intersimple sequence repeat markers were characterized for 17 tropical sprangletop populations collected from two separate regions mainly in Persian lime groves in Veracruz, Mexico. The whole-plant dose response together with shikimic acid assays indicated different levels of glyphosate resistance in those populations. Genetic diversity values (h) estimated using POPGENE ranged from 0.119 to 0.198 and 0.117 to 0.214 within susceptible and resistant populations, respectively. The average genetic diversity (HS) within the susceptible populations was 0.157, and the total genetic diversity (HT) was 0.218. The HS of the resistant populations was 0.144, and the HT was 0.186. The analysis of molecular variance based on the response to glyphosate indicated that most of the genetic variation was found within groups of susceptible and resistant populations (90% of the genetic variation), whereas 10% or less was among groups. The high level of genetic diversity between glyphosate-resistant tropical sprangletop populations from distant and adjacent locations is likely due to both short- and long-distance seed dispersal and independent evolutionary events in tropical sprangletop populations among Persian lime groves in Veracruz.

Keywords

Glyphosatetropical sprangletop, Leptochloa virgata (L.) P. BeauvPersian lime, Citrus latifolia TanDiversity within/among populationsgenetic diversityglyphosate resistance spreadISSR markerstropical sprangletop populationsresistance index
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 64 , Issue 4 , December 2016 , pp. 579 - 587
Copyright
Copyright © 2016 by the Weed Science Society of America 

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Footnotes

Associate Editor for this paper: Muthukumar V. Bagavathiannan, Texas A&M.

References

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