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Genetic diversity of jointed goatgrass (Aegilops cylindrica) determined with RAPD and AFLP markers

Published online by Cambridge University Press:  20 January 2017

Todd A. Pester ,
Sarah M. Ward ,
Ann L. Fenwick ,
Philip Westra  and
Scott J. Nissen
Sarah M. Ward
Affiliation:
Department of Soil and Crop Science, Colorado State University, Fort Collins, CO 80523
Ann L. Fenwick
Affiliation:
Department of Soil and Crop Science, Colorado State University, Fort Collins, CO 80523
Philip Westra
Affiliation:
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523
Scott J. Nissen
Affiliation:
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523
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Abstract

Two DNA molecular marker techniques were used to evaluate genetic diversity in 58 accessions of jointed goatgrass and 6 accessions of the related wild species barb goatgrass. Random amplified polymorphic DNA (RAPD) assays were performed on 8 U.S. and 50 Eurasian jointed goatgrass accessions using 30 random decamer primers. The frequency of scorable polymorphic bands within jointed goatgrass was 6 out of 90 (6.7%). Cluster analysis of RAPD data showed small genetic distances (values of 0.005 or less) among jointed goatgrass accessions. To validate the effectiveness of RAPD techniques to detect genetic diversity in tetraploid Aegilops species, six accessions of barb goatgrass were assayed using a subset of 20 decamer primers (from the original 30). RAPD data for barb goatgrass were pooled with jointed goatgrass data from the same primers. A total of 63 scorable bands were generated, of which 27 (43%) were polymorphic between two or more accessions. RAPD analysis readily distinguished between the two species and detected much greater levels of genetic diversity within barb goatgrass than between the jointed goatgrass accessions. Amplified fragment length polymorphism (AFLP) assays were performed on a subset of the 58 jointed goatgrass accessions, 3 U.S. and 13 Eurasian. These accessions were selected to represent a range in geographic diversity within our collection. Ten primer combinations generated 560 scorable bands of which 28 (5%) were polymorphic. Cluster analysis of AFLP data showed a slightly smaller range in genetic distance (0.0002 to 0.0022) among accessions compared with RAPD results; however, AFLPs distinguished among all but 2 of the 16 accessions surveyed. Although AFLP produced more scorable bands than RAPD did, both methods revealed limited genetic diversity in jointed goatgrass.

Keywords

Jointed goatgrass, Aegilops cylindrica L. AEGCYbarb goatgrass, Aegilops triuncialis L. AEGTRDNA fingerprintingweed genetics
Type
Research Article
Information
Weed Science , Volume 51 , Issue 3 , June 2003 , pp. 287 - 293
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Ball, D. A., Young, F. L., and Ogg, A. G. Jr. 1999. Selective control of jointed goatgrass (Aegilops cylindrica) with imazamox in herbicide-resistant wheat. Weed Technol. 13:77–82.Google Scholar
Bowden, W. M. 1959. The taxonomy and nomenclature of the wheats, barleys, and ryes and their wild relatives. Can. J. Bot. 37:657–684.Google Scholar
Burdon, J. J. and Marshall, D. R. 1981. Biological control and the reproductive mode of weeds. J. Appl. Ecol. 18:649–658.CrossRefGoogle Scholar
Gealy, D. R. 1987. Gas exchange properties of jointed goatgrass (Aegilops cylindrica). Weed Sci. 35:482–489.Google Scholar
Gealy, D. R. 1989. Response of gas exchange in jointed goatgrass (Aegilops cylindrica) to environmental conditions. Weed Sci. 37:562–569.Google Scholar
Jasieniuk, M. and Maxwell, B. D. 2001. Plant diversity: new insights from molecular biology and genomics technologies. Weed Sci. 49:257–265.Google Scholar
Johnston, C. O. and Parker, J. H. 1929. Aegilops cylindrica Host., a wheat-field weed in Kansas. Trans. Kans. Acad. Sci. 32:80–84.CrossRefGoogle Scholar
Kihara, H. 1954. Considerations on the evolution and distribution of Aegilops species based on the analyser method. Cytologia. 19:336–357.Google Scholar
Kimber, G. and Feldman, M. 1987. Wild Wheat. Columbia, MO: Special Rep. 353, College of Agriculture, University of Missouri-Columbia, pp. 60–61.Google Scholar
Mayfield, L. 1927. Goat grass—a weed pest of central Kansas wheat fields. Kans. Agric. Student. 7:40–41.Google Scholar
Mueller, U. G. and Wolfenbarger, L. L. 1999. AFLP genotyping and fingerprinting. Trends Ecol. Evol. 14:389–394.Google Scholar
Nei, M. 1972. Genetic distance between populations. Amer. Nat. 106:283–292.CrossRefGoogle Scholar
Okuno, K., Ebana, K., Voov, B., and Yoshida, H. 1998. Genetic diversity of central Asian and north Caucasian Aegilops species as revealed by RAPD markers. Gen. Res. Crop Evol. 45:389–304.CrossRefGoogle Scholar
Seefeldt, S. S., Zemetra, R., Young, F. L., and Jones, S. S. 1998. Production of herbicide-resistant jointed goatgrass (Aegilops cylindrica) × wheat (Triticum aestivum) hybrids in the field by natural hybridization. Weed Sci. 46:632–634.Google Scholar
Snyder, J. R., Mallory-Smith, C. A., Balter, S., Hansen, J. L., and Zemetra, R. S. 2000. Seed production on Triticum aestivum by Aegilops cylindrica hybrids in the field. Weed Sci. 48:588–593.Google Scholar
Tai, T. and Tanksley, S. D. 1990. A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue. Plant Mol. Biol. Rep. 8:297–303.Google Scholar
van Slageren, M. W. 1994. Wild Wheats: A Monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig. Wageningen Agricultural University Papers 7(94).Google Scholar
Vos, P., Hogers, R., Bleeker, M., et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23:4407–4414.CrossRefGoogle ScholarPubMed
Watanabe, N., Mastui, K., and Furuta, Y. 1994. Uniformity of the alphaamylase isozymes of Aegilops cylindrica introduced into North America: comparison with ancestral Eurasian accessions. Second Int. Wheat Symp., Logan, UT.Google Scholar
Williams, J.G.K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., and Tingey, S. V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531–6535.CrossRefGoogle ScholarPubMed
Zemetra, R. S., Hansen, J., and Mallory-Smith, C. 1998. Potential for gene transfer between wheat (Triticum aestivum) and jointed goatgrass (Aegilops cylindrica). Weed Sci. 46:313–317.Google Scholar
Zohary, D. 1965. Colonizer species in the wheat group. Pages 403–423 In Baker, H. G. and Stebbins, G. L., eds. The Genetics of Colonizing Species. New York: Academic Press.Google Scholar