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Effects of Picloram on Mitochondrial Swelling and ATPase

Published online by Cambridge University Press:  12 June 2017

In-Kook Chang  and
Chester L. Foy
In-Kook Chang
Affiliation:
Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia
Chester L. Foy
Affiliation:
Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia
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Abstract

Effects of 4-amino-3,5,6-trichloropicolinic acid (picloram) on mitochondrial volume changes and ATPase activity were studied with isolated barley (Hordeum vulgare L., var. Wong) and safflower (Carthamus tinctorius L., var. U. S. 10) mitochondria. Changes in mitochondrial volume and/or configuration of mitochondrial membranes were recorded in terms of changes in light scattering, which was expressed as absorbance. Picloram caused swelling of mitochondria previously contracted by ATP. Picloram-induced swelling was characterized by a later partial recovery of the mitochondria to a contracted state. Picloram did not affect succinate-induced contraction nor cause any additional swelling of the mitochondria in 0.3 M KCl medium. A high concentration (10−3 M) of picloram enhanced the activity of ATPase by 31%. This result was consistent with an increased rate of picloram-induced swelling in mitochondria contracted earlier by ATP. The action of picloram apparently differs from that of known mitochondrial swelling agents.

Type
Research Article
Information
Weed Science , Volume 19 , Issue 1 , January 1971 , pp. 54 - 58
Copyright
Copyright © Weed Science Society of America 

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