Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-24T04:31:01.386Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of Picloram on Mitochondrial Swelling and ATPase

Published online by Cambridge University Press:  12 June 2017

In-Kook Chang  and
Chester L. Foy
In-Kook Chang
Affiliation:
Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia
Chester L. Foy
Affiliation:
Department of Plant Pathology and Physiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia
Get access Rights & Permissions [Opens in a new window]

Abstract

Effects of 4-amino-3,5,6-trichloropicolinic acid (picloram) on mitochondrial volume changes and ATPase activity were studied with isolated barley (Hordeum vulgare L., var. Wong) and safflower (Carthamus tinctorius L., var. U. S. 10) mitochondria. Changes in mitochondrial volume and/or configuration of mitochondrial membranes were recorded in terms of changes in light scattering, which was expressed as absorbance. Picloram caused swelling of mitochondria previously contracted by ATP. Picloram-induced swelling was characterized by a later partial recovery of the mitochondria to a contracted state. Picloram did not affect succinate-induced contraction nor cause any additional swelling of the mitochondria in 0.3 M KCl medium. A high concentration (10−3 M) of picloram enhanced the activity of ATPase by 31%. This result was consistent with an increased rate of picloram-induced swelling in mitochondria contracted earlier by ATP. The action of picloram apparently differs from that of known mitochondrial swelling agents.

Type
Research Article
Information
Weed Science , Volume 19 , Issue 1 , January 1971 , pp. 54 - 58
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Armarego, W. L. F., Canny, M. J., and Cox, S. A. 1959. Metal-chelating properties of plant-growth substances. Nature 183: 1176–1177.CrossRefGoogle Scholar
2. Blondin, G. A., Vail, W. J., and Green, D. E. 1969. The mechanism of mitochondrial swelling. II. Pseudoenergized swelling in the presence of alkali metal salts. Arch. Biochem. Biophys. 129:158–172.CrossRefGoogle ScholarPubMed
3. Bonner, W. D. Jr. 1967. A general method for the preparation of plant mitochondria, p. 126–133. In Estabrook, R. W. and Pullman, M. E. [eds.] Methods in Enzymology, Vol. X, Academic Press, Inc., New York.Google Scholar
4. Bottrill, D. E. 1965. Studies on the uncoupling action of 2,4-dichlorophenoxyacetic acid as a basis for growth inhibition in corn roots. Diss. Abstr. 26:57.Google Scholar
5. Chappell, J. B. and Greville, B. D. 1963. The influence of the composition of the suspending medium on the properties of mitochondria. Symp. Biochem. Soc. (Cambridge) 23:39–65.Google Scholar
6. Collins, R. P. and Scheffer, R. P. 1958. Respiratory responses and systemic effects in fusarium-infected tomato plants. Phytopathology 48:349–355.Google Scholar
7. Earnshaw, M. J. and Truelove, B. 1968. Swelling and contraction of Phaseolus hypocotyl mitochondria. Plant Physiol. 43:121–129.CrossRefGoogle ScholarPubMed
8. Foy, C. L. and Penner, D. 1965. Effect of inhibitors and herbicides on tricarboxylic acid cycle substrate oxidation by isolated cucumber mitochondria. Weeds 13:226–231.CrossRefGoogle Scholar
9. Green, D. E., Asai, J., Harris, R. A., and Penniston, J. T. 1968. Conformational basis of energy transformations in membrane systems. III. Configurational changes in the mitochondrial inner membrane induced by changes in functional states. Arch. Biochem Biophys 125:684–705.CrossRefGoogle Scholar
10. Green, D. E., Allmann, D. W., Harris, R. A., and Tan, W. C. 1968. Enzyme localization in the inner and outer mitochondrial membranes. Biochem. Biophys. Res. Commun. 13:368–378.CrossRefGoogle Scholar
11. Harris, R. A., Penniston, J. T., Asai, J., and Green, D. E. 1968. The conformational basis of energy conservation in membrane systems. II. Correlation between conformational change and functional states. Proc. Nat. Acad. Sci. U. S. 59:830–837.CrossRefGoogle ScholarPubMed
12. Harris, R. A., Asbell, M. A., and Green, D. E. 1969. Correlation between light-scattering changes and the energy state of phosphorylating submitochondrial particles. Arch. Biochem. Biophys. 131:316–318.CrossRefGoogle Scholar
13. Jacobs, E. E., Jacob, M., Sanadi, D. R., and Bradley, L. B. 1956. Uncoupling of oxidative phosphorylation by cadmium ion. J. Biol. Chem. 223:147–156.CrossRefGoogle Scholar
14. Kenefick, D. G. and Hanson, J. B. 1966. Contracted state as an energy source for Ca binding and Ca + inorganic phosphate accumulation by corn mitochondria. Plant Physiol. 41:1601–1609.CrossRefGoogle ScholarPubMed
15. Lee, T. T. 1968. Effect of ozone on swelling of tobacco mitochondria. Plant Physiol. 43:133–139.CrossRefGoogle ScholarPubMed
16. Lehninger, A. L. 1962. Water uptake and extrusion by mitochondria in relation to oxidative phosphorylation. Physiol. Rev. 42:467–517.CrossRefGoogle ScholarPubMed
17. Lotlikar, P. D., Remmert, L. F., and Freed, V. H. 1968. Effects of 2,4-D and other herbicides on oxidative phosphorylation in mitochondria from cabbage. Weed Sci. 16:161–165.CrossRefGoogle Scholar
18. Lyons, J. M. and Pratt, H. K. 1964. An effect of ethylene on swelling of isolated mitochondria. Arch. Biochem. Biophys. 104:318–324.CrossRefGoogle ScholarPubMed
19. Penniston, J. R. and Green, D. E. 1968. The conformational basis of energy transformations in membrane systems. IV. Energized states and pinocytosis in erythrocyte ghosts. Arch. Biochem. Biophys. 128:339–350.CrossRefGoogle ScholarPubMed
20. Sanwal, B. D. 1959. Mechanism of action and metabolism of the toxins, p. 1012–1017. In Recent Advances in Botany (9th Int. Bot. Congr., Montreal) Vol. 2, University of Toronto Press, Toronto.Google Scholar
21. Stoner, C. D. and Hanson, J. B. 1966. Swelling and contraction of corn mitochondria. Plant Physiol. 41:255–266.CrossRefGoogle ScholarPubMed
22. Sumner, J. B. 1944. A method for the colorimetric determination of phosphorus. Science 100:413–414.CrossRefGoogle ScholarPubMed
23. Switzer, C. M. 1957. Effects of herbicides and related chemicals on oxidation and phosphorylation by isolated soybean mitochondria. Plant Physiol. 32:42–44.CrossRefGoogle ScholarPubMed
24. Truelove, B. and Hanson, J. B. 1966. Calcium-activated phosphate uptake in contracting corn mitochondria. Plant Physiol. 41:1004–1013.CrossRefGoogle ScholarPubMed