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Effects of Herbicides on Growth and Extractable Phenylalanine Ammonia-Lyase Activity in Light- and Dark-Grown Soybean (Glycine max) Seedlings

Published online by Cambridge University Press:  12 June 2017

Robert E. Hoagland  and
Stephen O. Duke
Robert E. Hoagland
Affiliation:
South. Weed Sci. Lab., U.S. Dep. Agric., Sci. Ed. Admin., Agric. Res., Stoneville, MS 38776
Stephen O. Duke
Affiliation:
South. Weed Sci. Lab., U.S. Dep. Agric., Sci. Ed. Admin., Agric. Res., Stoneville, MS 38776
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Abstract

Effects of 16 herbicides representing 14 herbicide classes on growth and extractable phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) were examined in light- and dark-grown soybean [Glycine max (L.) Merr. ‘Hill’] seedlings. High purity (96 to 100%) herbicides were supplied via aqueous culture at various concentrations: 0.5 mM amitrole (3-amino-s-triazole), 0.1 mM atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine], 0.07 mM diclofop-methyl {methyl ester of 2-[4-(2,4-dichlorophenoxy)phenoxy] propanoicacid}, 0.5 mM DSMA (disodium methanearsonate), 0.2 mM fenuron (1,1-dimethyl-3-phenylurea), 0.05 mM fluridone {1-methyl-3-phenyl-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone}, 0.5 mM MH (1,2-dihydro-3,6-pyridazinedione), 0.5 mM metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one], 1.8 μM nitralin [4-(methylsulfonyl)-2,6-dinitro-N,N-dipropylaniline], 0.5 mM norflurazon [4-chloro-5-(methylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone], 0.05 mM paraquat (1,1′-dimethyl-4,4′-bipyridinium ion), 0.15 mM perfluidone {1,1,1-trifluoro-N-[2-methyl-4-(phenylsulfonyl)phenyl] methanesulfonamide}, 0.2 mM propanil (3′,4′-dichloropropionanilide), 0.1 mM propham (isopropyl carbanilate), 0.5 mM TCA (trichloroacetic acid), and 0.05 mM 2,4-D [(2,4-dichlorophenoxy)acetic acid]. Dark-grown soybean seedlings (3-day-old) were transferred to control solutions (2 mM CaSO4) or to herbicide solutions (in 2 mM CaSO4) and grown at 25 C in continuous white light (200 μE•m-2•s-1) or continuous darkness until harvested 24 or 48 h after transfer. After 48 h, growth (fresh weight, dry weight, elongation) was inhibited by most of the chemicals. Other signs of toxicity (necrosis, secondary root stunting, and root tip swelling) were noted for some treatments. Roots were most affected, although hypocotyls were generally not changed. Hypocotyl elongation was stimulated by atrazine, fluridone, and norflurazon after 48 h light. Extractable PAL activity from soybean axes was decreased by atrazine, fenuron, metribuzin, norflurazon, propanil, propham, and 2,4-D. Amitrole and paraquat were the only herbicides that increased extractable PAL activity. Other compounds tested had no effect on the enzyme. None of the herbicides significantly affected in vitro PAL activity.

Keywords

Mechanism of actionphenolic metabolismPAL [EC 4.3.1.5.]glyphosate
Type
Research Article
Information
Weed Science , Volume 29 , Issue 4 , July 1981 , pp. 433 - 439
Copyright
Copyright © 1981 by the Weed Science Society of America 

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References

Literature Cited

1. Alibert, G., Ranjeva, R., and Boudet, A. M. 1977. Organisation subcellulaire des voies de synthese des composes phenoliques. Physiol. Veg. 15:279–301.Google Scholar
2. Amrhein, N. and Zenk, M. H. 1971. Untersuchungen zur rolle der phenylalanin-ammonium-lyase (PAL) bei der regulation der flavonoid-synthese in buckweizen (Fagopyrum esculentum Moench.). Z. Pflanzenphysiol. 64:145–168.Google Scholar
3. Ben-Aziz, A. and Koren, E. 1974. Interference in carotenogenesis as a mechanism of action of the pyridazinone herbicide Sandoz 6706. Plant Physiol. 54:916–920.Google Scholar
4. Billett, E. E., Wallace, W., and Smith, H. 1978. A specific and reversible macromolecular inhibitor of phenylalanine ammonia-lyase and cinnamic acid-4-hydroxylase in gherkins. Biochim. Biophys. Acta 24:219–230.CrossRefGoogle Scholar
5. Blume, D. E. and McClure, J. W. 1978. Photocontrol of phenylalanine ammonia-lyase in barley seedlings treated with pyridazinone inhibitors of chloroplast development. Phytochemistry 17:1545–1547.Google Scholar
6. Camm, E. L. and Towers, G. H. N. 1973. Phenylalanine ammonia-lyase. Phytochemistry 12:961–973.Google Scholar
7. Camm, E. L. and Towers, G. H. N. 1977. Phenylalanine ammonia-lyase. Pages 169–188 in Reinholt, L., Harborne, J. B., and Swain, T., eds. Progress in Phytochemistry, Vol. 4. Permagon Press, New York.Google Scholar
8. Cole, D. J., Dodge, A. D., and Caseley, J. C. 1980. Some biochemical effects of glyphosate on plant meristems. J. Exp. Bot. 31:1665–1674.CrossRefGoogle Scholar
9. Creasy, L. L. 1968. The increase in phenylalanine ammonia-lyase activity in strawberry leaf discs and its correlation with flavanoid synthesis. Phytochemistry 7:441–446.Google Scholar
10. Creasy, L. L. 1976. Phenylalanine ammonia-lyase-inactivating system in sunflower leaves. Phytochemistry 15:673–675.Google Scholar
11. Creasy, L. L. and Zucker, M. 1974. Phenylalanine ammonia-lyase and phenolic metabolism. Pages 1–19 in Runeckles, V. C. and Conn, E. E., eds. Recent Adv. Phytochem., Vol. 8, Academic Press, New York.Google Scholar
12. Davies, M. E. 1972. Effects of auxin on polyphenol accumulation and the development of phenylalanine ammonia-lyase activity in dark-grown suspension cultures of Paul's Scarlet Rose. Planta 104.66–77.CrossRefGoogle Scholar
13. Duke, S. O. and Hoagland, R. E. 1978. Effects of glyphosate on metabolism of phenolic compounds. I. Induction of phenylalanine ammonia-lyase activity in dark-grown maize roots. Plant Sci. Lett. 11. 185–190.Google Scholar
14. Duke, S. O. and Hoagland, R. E. 1981. Effects of glyphosate on the metabolism of phenolic compounds: VII. Root-fed amino acids and glyphosate toxicity in soybean (Glycine max) seedlings. Weed Sci. 29: in press.CrossRefGoogle Scholar
15. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1979. Effects of glyphosate on metabolism of phenolic compounds. IV. Phenylalanine ammonia-lyase activity, free amino acids, and soluble hydroxyphenolic compounds in axes of light-grown soybeans. Physiol. Plant. 46:307–317.Google Scholar
16. Duke, S. O., Hoagland, R. E., and Elmore, C. D. 1980. Effects of glyphosate on metabolism of phenolic compounds. V. L-α-aminooxy-β-phenylpropionic acid and glyphosate effects on phenylalanine ammonia-lyase in soybean seedlings. Plant Physiol. 65:17–21.CrossRefGoogle ScholarPubMed
17. Duke, S. O. and Naylor, A. W. 1974. Effects of light on phenylalanine ammonia-lyase activity in dark-grown Zea mays (L.) seedlings. Plant Sci. Lett. 2:289–293.Google Scholar
18. Engelsma, G. 1973. Induction of phenylalanine ammonia-lyase by dichlobenil in gherkin seedlings. Acta Bot. Neerl. 22:49–54.CrossRefGoogle Scholar
19. Engelsma, G. and van Bruggen, J. M. H. 1971. Ethylene production and enzyme induction in excised plant tissues. Plant Physiol. 48:94–96.Google Scholar
20. Farrington, J. A., Ebert, M., Land, E. J., and Fletcher, K. 1973. Bipyridylium quaternary salts and related compounds. V. Pulse radiolysis studies of the reaction of paraquat radical with oxygen. Implications for the mode of action of bipyridyl herbicides. Biochim. Biophys. Acta 314:372–381.CrossRefGoogle ScholarPubMed
21. Havir, E. A. and Hanson, K. R. 1970. L-phenylalanine ammonia-lyase (potato tubers). Pages 575–581 in Tabor, H. and Tabor, C. W., eds. Methods in Enzymology, Vol. 17. Academic Press, New York.Google Scholar
22. Hilton, J. L., St. John, J. B., Christiansen, M. N., and Norris, K. H. 1971. Interactions of lipoidal materials and a pyridazinone inhibitor of chloroplast development. Plant Physiol. 48:171–177.Google Scholar
23. Hoagland, R. E. 1980. Effects of glyphosate on metabolism of phenolic compounds. VI. Effects of glyphosine and glyphosate metabolites on phenylalanine ammonia-lyase activity, growth, and protein, chlorophyll and anthocyanin levels in soybean (Glycine max) seedlings. Weed Sci. 28:393–400.CrossRefGoogle Scholar
24. Hoagland, R. E. and Duke, S. O. 1979. Effects of herbicides on growth and soluble protein, hydroxyphenolic compound and anthocyanin levels in light- and dark-grown (Glycine max (L.) Merr. seedlings. Plant Physiol. Suppl. 63:106.Google Scholar
25. Hoagland, R. E., Duke, S. O., and Elmore, C. D. 1978. Effects of glyphosate on metabolism of phenolic compounds. II. Influence on soluble hydroxyphenolic compounds, free amino acid and soluble protein levels in dark-grown maize roots. Plant Sci. Lett. 13:291–299.Google Scholar
26. Hoagland, R. E., Duke, S. O., and Elmore, C. D. 1979. The effects of glyphosate on metabolism of phenolic compounds. III. Phenylalanine ammonia-lyase activity, free amino acids, soluble protein and hydroxyphenolic compounds in axes of dark-grown soybeans. Physiol. Plant. 46:357–366.Google Scholar
27. Hoagland, R. E. and Graf, G. 1974. The purification and properties of an amidohydrolase from soybean. Can. J. Biochem. 52: 903–910.Google Scholar
28. Innerarity, L. T., Smith, E. C., and Wender, S. H. 1972. Indolacetic acid inhibition of a phenylalanine ammonia-lyase preparation from suspension cultures of WR-132 tobacco. Phytochemistry 11:83–88.Google Scholar
29. Jabben, M. and Deitzer, G. F. 1979. Effects of the herbicide San 9789 on photomorphogenic responses. Plant Physiol. 63: 481–485.Google Scholar
30. Jangaard, N. O. 1974. The characterization of phenylalanine ammonia-lyase from several plant species. Phytochemistry 13: 1765–1768.Google Scholar
31. Jangaard, N. O. 1974. The effect of herbicides, plant growth regulators and other compounds on phenylalanine ammonia-lyase activity. Phytochemistry 13:1769–1775.Google Scholar
32. Jaworski, E. G. 1972. Mode of action of N-(phosphonomethyl) glycine: inhibition of aromatic amino acid biosynthesis. J. Agric. Food Chem. 20:1195–1198.Google Scholar
33. Loffelhardt, W., Ludwig, B., and Kindl, H. 1973. Thylakoidgebunde L-phenylalanine-ammonia-lyase. Hoppe-Seyler's Z. Physiol. Chem. 354:1006–1014.Google Scholar
34. Margna, U. 1977. Control at the level of substrate supply – an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry 15:419–426.Google Scholar
35. Moreland, D. E. and Hill, K. L. 1962. Interference of herbicides with the Hill reaction of isolated chloroplasts. Weeds 10:229–236.CrossRefGoogle Scholar
36. Murphy, J. B. and Kies, M. W. 1960. Note on a spectrophotometric determination of proteins in dilute solutions. Biochim. Biophys. Acta 45:382–384.CrossRefGoogle Scholar
37. Nishizawa, A. N., Wolosiuk, R. A., and Buchanan, B. B. 1979. Chloroplast phenylalanine ammonia-lyase from spinach leaves. Evidence for light-mediated regulation via the ferredoxin/thioredoxin system. Planta 145:7–12.CrossRefGoogle ScholarPubMed
38. Roisch, U. and Lingens, F. 1974. Effects of the herbicide N-(phosphonomethyl)glycine on the biosynthesis of aromatic amino acids. Angew. Chem. 86:408.Google Scholar
39. Saunders, J. A. and McClure, J. W. 1975. Phytochrome controlled phenylalanine ammonia-lyase in Hordeum vulgare plastids. Phytochemistry 14:1285–1289.CrossRefGoogle Scholar
40. St. John, J. B. and Hilton, J. L. 1976. Structure versus activity of substituted pyridazinones as related to mechanism of action. Weed Sci. 24:579–582.Google Scholar
41. Steinrücken, H. C. and Amrhein, N. 1980. The herbicide glyphosate is a potent inhibitor of 5-enolpyruvylshikimic acid-3-phosphate synthase. Biochem. Biophys. Res. Commun. 94:1207–1212.Google Scholar
42. Tomé, F., Scarpini, E., and Bellini, E. 1978. The effect of reduced oxygen supply on the development of phenylalanine ammonia-lyase activity in radish cotyledons. Z. Pflanzenphysiol. 89:21–27.CrossRefGoogle Scholar
43. Vallee, J. C., Paynot, M., Martin, C., Vansuyt, G., and Prevost, J. 1975. Action de molecules et proprietes hormonales sur l'activite phenylalanine ammoniac lyase. Phytochemistry 14:2147–2151.Google Scholar
44. Zucker, M. 1972. Light and enzymes. Annu. Rev. Plant Physiol. 23:1333–156.CrossRefGoogle Scholar