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Effects of Difenzoquat on Photoreactions and Respiration in Wheat (Triticum aestivum) and Wild Oat (Avena fatua)

Published online by Cambridge University Press:  12 June 2017

Blaik P. Halling  and
Richard Behrens
Blaik P. Halling
Affiliation:
Dep. Agron. and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108
Richard Behrens
Affiliation:
Dep. Agron. and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108
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Abstract

Experiments were conducted with isolated protoplasts of wild oat (Avena fatua L. # AVEFA) and isolated chloroplasts of wild oat and wheat (Triticum aestivum L.), to determine if the methyl sulfate salt of difenzoquat (1,2-dimethyl-3,5-diphenyl-1H-pyrazolium) might influence photoreactions in these species. Difenzoquat did not affect CO2 fixation, uncoupled electron transport, or proton uptake. At concentrations of 0.5 mM and 1 mM, difenzoquat caused a slight, but statistically significant, inhibition of photophosphorylation. Experiments assaying coupled electron transport indicated that inhibition of photophosphorylation occurred not through uncoupling, but by an energy-transfer inhibition. This same effect was observed in isolated mitocondria of both species, with about 50% inhibition of state 3 respiration rates occurring with 10 μM difenzoquat. However, no important differentials were observed in the relative susceptibilities of wheat and wild oat mitochondria. Difenzoquat also functioned as a weak autooxidizing electron acceptor in photosynthetic electron transport. Therefore, difenzoquat-induced leaf chlorosis and necrosis may result from a bipyridilium-type electron acceptor activity if sufficient herbicide is absorbed.

Keywords

ChloroplastprotoplastmitochondriaAVEFA
Type
Research Article
Information
Weed Science , Volume 31 , Issue 5 , September 1983 , pp. 693 - 699
Copyright
Copyright © 1983 Weed Science Society of America 

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References

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